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AB124893

Anti-ING1 antibody [EPR6788]

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(3 Publications)

Rabbit Recombinant Monoclonal ING1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Mouse, Human samples. Cited in 3 publications.

View Alternative Names

Inhibitor of growth protein 1, ING1

2 Images
Flow Cytometry (Intracellular) - Anti-ING1 antibody [EPR6788] (AB124893)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ING1 antibody [EPR6788] (AB124893)

Intracellular flow cytometric analysis of ab124893, at 1/10 dilution, staining ING1 in permeabilised NIH/3T3cells (red). Isotype control (Green).

Western blot - Anti-ING1 antibody [EPR6788] (AB124893)
  • WB

Unknown

Western blot - Anti-ING1 antibody [EPR6788] (AB124893)

All lanes:

Western blot - Anti-ING1 antibody [EPR6788] (ab124893) at 1/1000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

MCF-7 lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Lane 5:

C6 lysate at 10 µg

Lane 6:

RAW 264.7 lysate at 10 µg

Lane 7:

PC-12 lysate at 10 µg

Lane 8:

NIH/3T3 lysate at 10 µg

Predicted band size: 47 kDa

false

  • Carrier free

    Anti-ING1 antibody [EPR6788] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6788

Isotype

IgG

Carrier free

No

Reacts with

Rat, Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ING1 also known as Inhibitor of Growth Protein 1 is a tumor suppressor protein with a mass of approximately 33 kDa. It functions mechanically by interacting with chromatin and transcriptional machinery to regulate cell growth apoptosis and DNA repair. ING1 localizes both in the nucleus and cytoplasm showing expression in various tissues but particularly in those with high proliferation rates.
Biological function summary

As part of the chromatin remodeling complex ING1 contributes to the regulation of gene expression by promoting histone acetylation. It affects the cellular response to stress and helps maintain genomic stability. Through interactions with other proteins in the complex ING1 influences cell cycle checkpoints and can induce apoptosis when DNA damage is irreparable.

Pathways

Several important cellular signaling cascades involve ING1. Notably it plays a role in the p53 signaling pathway where it collaborates with the tumor protein p53 to enhance transcriptional activation of downstream genes associated with growth arrest and apoptosis. Additionally ING1 participates in the PI3K/AKT pathway influencing cell survival and metabolism by modulating PI3K-mediated phosphorylation events.

Studies indicate that ING1 has significant links to cancer such as breast and colorectal cancer. Loss or mutation of ING1 disrupts its tumor suppressive functions leading to unchecked cellular proliferation. Moreover its interaction with the protein TP53 highlights its relevance in tumorigenesis as disruptions can impair p53-mediated tumor suppressive pathways further contributing to cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.
See full target information ING1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell 178:731-747.e16 PubMed31257032

2019

Deciphering the "mA Code" via Antibody-Independent Quantitative Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Angel Garcia-Campos,Sarit Edelheit,Ursula Toth,Modi Safra,Ran Shachar,Sergey Viukov,Roni Winkler,Ronit Nir,Lior Lasman,Alexander Brandis,Jacob H Hanna,Walter Rossmanith,Schraga Schwartz

Molecular medicine reports 17:2535-2542 PubMed29207098

2017

FOXO1 is crucial in glioblastoma cell tumorigenesis and regulates the expression of SIRT1 to suppress senescence in the brain.

Applications

Unspecified application

Species

Unspecified reactive species

Han Yan,Anhua Wu

Scientific reports 5:11542 PubMed26100219

2015

Latent progenitor cells as potential regulators for tympanic membrane regeneration.

Applications

IHC-Fr

Species

Rat

Seung Won Kim,Jangho Kim,Hoon Seonwoo,Kyung-Jin Jang,Yeon Ju Kim,Hye Jin Lim,Ki-Taek Lim,Chunjie Tian,Jong Hoon Chung,Yun-Hoon Choung
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com