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AB248045

Anti-ING1 antibody [EPR6788] - BSA and Azide free

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Rabbit Recombinant Monoclonal ING1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

View Alternative Names

Inhibitor of growth protein 1, ING1

2 Images
Flow Cytometry (Intracellular) - Anti-ING1 antibody [EPR6788] - BSA and Azide free (AB248045)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ING1 antibody [EPR6788] - BSA and Azide free (AB248045)

This data was developed using ab124893, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of ab124893, at 1/10 dilution, staining ING1 in permeabilised NIH/3T3cells (red). Isotype control (Green).

Western blot - Anti-ING1 antibody [EPR6788] - BSA and Azide free (AB248045)
  • WB

Unknown

Western blot - Anti-ING1 antibody [EPR6788] - BSA and Azide free (AB248045)

This data was developed using ab124893, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ING1 antibody [EPR6788] (<a href='/en-us/products/primary-antibodies/ing1-antibody-epr6788-ab124893'>ab124893</a>) at 1/1000 dilution

Lane 1:

HepG2 lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

MCF-7 lysate at 10 µg

Lane 4:

Jurkat lysate at 10 µg

Lane 5:

C6 lysate at 10 µg

Lane 6:

RAW 264.7 lysate at 10 µg

Lane 7:

PC-12 lysate at 10 µg

Lane 8:

NIH/3T3 lysate at 10 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6788

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248045 is the carrier-free version of ab124893.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ING1 also known as Inhibitor of Growth Protein 1 is a tumor suppressor protein with a mass of approximately 33 kDa. It functions mechanically by interacting with chromatin and transcriptional machinery to regulate cell growth apoptosis and DNA repair. ING1 localizes both in the nucleus and cytoplasm showing expression in various tissues but particularly in those with high proliferation rates.
Biological function summary

As part of the chromatin remodeling complex ING1 contributes to the regulation of gene expression by promoting histone acetylation. It affects the cellular response to stress and helps maintain genomic stability. Through interactions with other proteins in the complex ING1 influences cell cycle checkpoints and can induce apoptosis when DNA damage is irreparable.

Pathways

Several important cellular signaling cascades involve ING1. Notably it plays a role in the p53 signaling pathway where it collaborates with the tumor protein p53 to enhance transcriptional activation of downstream genes associated with growth arrest and apoptosis. Additionally ING1 participates in the PI3K/AKT pathway influencing cell survival and metabolism by modulating PI3K-mediated phosphorylation events.

Studies indicate that ING1 has significant links to cancer such as breast and colorectal cancer. Loss or mutation of ING1 disrupts its tumor suppressive functions leading to unchecked cellular proliferation. Moreover its interaction with the protein TP53 highlights its relevance in tumorigenesis as disruptions can impair p53-mediated tumor suppressive pathways further contributing to cancer development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.
See full target information ING1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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