AB249807

Anti-ING2 antibody [EPR4597-5] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ING2 antibody. Carrier free. Suitable for WB, IP and reacts with Recombinant full length protein, Human, Mouse samples.

View Alternative Names

ING1L, ING2, Inhibitor of growth protein 2, Inhibitor of growth 1-like protein, p32, p33ING2, ING1Lp

2 Images

Supplier Data

Western blot - Anti-ING2 antibody [EPR4597-5] - BSA and Azide free (AB249807)

This data was developed using ab174305, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ING2 antibody [EPR4597-5] (<a href='/en-us/products/primary-antibodies/ing2-antibody-epr4597-5-ab174305'>ab174305</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HT-1080 cell lysate at 10 µg

Lane 3:

Saos-2 cell lysate at 10 µg

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-ING2 antibody [EPR4597-5] - BSA and Azide free (AB249807)

This data was developed using ab174305, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ING2 antibody [EPR4597-5] (<a href='/en-us/products/primary-antibodies/ing2-antibody-epr4597-5-ab174305'>ab174305</a>) at 1/1000 dilution

All lanes:

ING2 recombinant protein (10ng)

Predicted band size: 33 kDa

false

Unconjugated

Anti-ING2 antibody [EPR4597-5]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4597-5

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab249807 is the carrier-free version of ab174305.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ING2 also known as Inhibitor of Growth Protein 2 is a nuclear protein with a molecular weight of approximately 33 kDa. It expresses highly in tissues such as the prostate placenta and lung. Nuclear localization signals and PHD-finger motifs characterize its structure allowing it to bind to chromatin and influence gene expression. As a member of the ING family ING2 plays a role in regulating various cellular processes through its presence in different cellular compartments.

Biological function summary

ING2 functions as a tumor suppressor by controlling the transcription of genes involved in cell cycle regulation and apoptosis. It interacts with histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes contributing to chromatin remodeling. As part of the Sin3A-HDAC complex ING2 has the ability to repress transcription influencing the expression of genes important for maintaining cellular homeostasis and preventing oncogenesis.

Pathways

The ING2 protein actively participates in the p53 signaling pathway as well as the MAPK signaling pathway. Its interaction with p53 leads to the enhancement of p53-mediated transcriptional activation which promotes cell cycle arrest and apoptosis. ING2 also interacts with proteins like TP53 and EP300 modulating the cellular responses to stress signals and contributing to cell survival and repair mechanisms.

The involvement of ING2 has been linked to cancer and neurodegenerative disorders. In cancer downregulation or mutation of ING2 disrupts the p53 pathway leading to impaired tumor suppression. ING2's interaction with TP53 and its tumor suppressor role highlights its importance in cancer biology. Furthermore in neurodegenerative disorders ING2's dysregulation can influence neuronal survival pathways potentially affecting proteins like p53 and EP300 therefore altering neuronal function and contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).

See full target information ING2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ING2 antibody [EPR4597-5] - BSA and Azide free

Western blot - Anti-ING2 antibody [EPR4597-5] - BSA and Azide free (AB249807)

Western blot - Anti-ING2 antibody [EPR4597-5] - BSA and Azide free (AB249807)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise