JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB189861

Anti-ING3 antibody

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal ING3 antibody. Suitable for WB, ICC/IF and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ING3.

View Alternative Names

HSPC301, ING3, Inhibitor of growth protein 3, p47ING3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ING3 antibody (AB189861)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ING3 antibody (AB189861)

Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab189861. Blue DAPI for nuclear staining.

Western blot - Anti-ING3 antibody (AB189861)
  • WB

Supplier Data

Western blot - Anti-ING3 antibody (AB189861)

All lanes:

Western blot - Anti-ING3 antibody (ab189861) at 1/500 dilution

Lane 1:

293T cell extracts

Lane 2:

SW620 cell extracts

Lane 3:

HeLa cell extracts

Lane 4:

Rat testis cell extracts

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ING3.

Q9NXR8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ING3 protein also known as inhibitor of growth family member 3 is a nuclear protein with a molecular mass of approximately 47 kDa. ING3 belongs to the ING gene family and acts as a tumor suppressor by modulating chromatin structure and regulating gene expression. It is expressed in various tissues including the liver kidney and heart and plays a critical role in cellular stress responses. ING3 interacts with several components of the histone acetyltransferase (HAT) complex regulating processes like cell growth apoptosis and DNA repair.
Biological function summary

ING3 influences gene transcription and chromatin remodeling as a part of the NuA4 histone acetyltransferase complex. This complex is involved in the acetylation of histone H4 and H2A facilitating an open chromatin structure that permits transcriptional activation. ING3 is important in the regulation of cell cycle progression and apoptosis. It modulates p53 and other key cellular pathways underpinning its role in maintaining normal cellular homeostasis and preventing cancerous transformations.

Pathways

ING3's function supports significant biological processes like DNA repair and apoptosis. It is part of the p53 signaling pathway which plays a pivotal role in the cellular response to DNA damage. ING3 acts alongside proteins such as p21 and MDM2 in the p53 pathway. Furthermore ING3 interfaces with the MAPK/ERK signaling pathway contributing to its regulation of cell division and survival. Through these pathways ING3 helps orchestrate a balanced response to cellular stress and damage.

Researchers associate ING3 with cancers particularly breast and liver cancers. Studies indicate that downregulation or mutation of ING3 leads to disrupted cell cycle control and increased tumorigenesis. Furthermore ING3's interaction with proteins such as p53 emphasizes its critical role in tumor suppression. By modulating pathways linked to proliferation and apoptosis ING3 provides potential targets for therapeutic intervention in cancer treatments highlighting its relevance in disease management and research.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
See full target information ING3
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:99 PubMed40493284

2025

SENP3 alleviates osteoporosis via promoting SIRT3 transcription through the increase of DLX2 stability via SUMO2/3.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Bu,Xuezheng Xu,Yi Luo,Jianfan Liu,Feng Zhou

Cancer gene therapy 25:240-247 PubMed29855536

2018

Inhibitor of growth 3 induces cell death by regulating cell proliferation, apoptosis and cell cycle arrest by blocking the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Song Zhao,Long Wang,Chunmei Zhang,Yu Deng,Bai Zhao,Yuxin Ren,Yingmei Fu,Xianzhi Meng

The Journal of biological chemistry 281:11887-93 PubMed16520380

2006

ING3 promotes UV-induced apoptosis via Fas/caspase-8 pathway in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yemin Wang,Gang Li
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB112043

Anti-EPC1 antibody

Immunoprecipitation - Anti-EPC1 antibody (AB112043)

  • 0
  • 0
Primary Antibodies

AB191536

Anti-PAG608 antibody

Western blot - Anti-PAG608 antibody (AB191536)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB75851

Anti-Cullin 3/CUL-3 antibody [EPR3196Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (AB75851)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com