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AB108621

Anti-ING4 antibody [EP3804]

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(4 Publications)

Rabbit Monoclonal ING4 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

My036, ING4, Inhibitor of growth protein 4, p29ING4

1 Images
Western blot - Anti-ING4 antibody [EP3804] (AB108621)
  • WB

Supplier Data

Western blot - Anti-ING4 antibody [EP3804] (AB108621)

All lanes:

Western blot - Anti-ING4 antibody [EP3804] (ab108621) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

  • Carrier free

    Anti-ING4 antibody [EP3804] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3804

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ING4 also known as Inhibitor of Growth Family Member 4 is a nuclear protein with a molecular mass of approximately 29 kDa. This protein mainly localizes to the nucleus and is expressed in various tissues including the brain and liver. ING4 contains a plant homeodomain (PHD) finger motif which suggests its involvement in chromatin-mediated transcriptional regulation. As a regulator of gene expression ING4 influences cell growth and apoptosis by interacting with histones to affect chromatin structure and function.
Biological function summary

The ING4 protein plays a significant role in tumor suppression. It forms part of a larger protein complex by associating with components like the nucleosome remodeling and histone deacetylase (NuRD) complex. By binding to specific histone modifications ING4 influences transcriptional regulation of several genes that control cell cycle and apoptosis. This activity helps maintain genomic stability and prevent cellular transformation establishing ING4 as a guardian against oncogenic processes.

Pathways

ING4 integrates into cellular machinery that governs the NF-κB signaling pathway and the p53 pathway. These pathways are critical for controlling inflammation and cellular stress responses. Within the NF-κB pathway ING4 interacts with proteins such as IκBα modulating inflammatory cytokine production. In the context of the p53 pathway ING4 acts together with p53 enhancing its tumor suppressor functions through transcriptional activation of p53-responsive genes.

ING4 has a notable association with cancer particularly glioblastoma and breast cancer. Reduced expression or alterations in ING4 have been linked to these malignancies where it functions to suppress the aggressive characteristics of tumor cells. ING4 also connects with proteins like HIF-1α in pathways that regulate cellular responses to hypoxia further implicating its role in tumor biology. Understanding ING4's interactions and effects offers potential for developing therapeutic strategies targeting cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4 (PubMed : 16387653). Through chromatin acetylation it may function in DNA replication (PubMed : 16387653). May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation (PubMed : 15251430, PubMed : 15528276). Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA (PubMed : 15029197). May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC (PubMed : 15029197). Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1) (PubMed : 15897452). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity).
See full target information ING4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:845097 PubMed35496267

2022

ING4 Promotes Stemness Enrichment of Human Renal Cell Carcinoma Cells Through Inhibiting DUSP4 Expression to Activate the p38 MAPK/type I IFN-Stimulated Gene Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Tang,Xinyue Yang,Qing Wang,Haoyu Huang,Qinzhi Wang,Min Jiang,Chunluan Yuan,Yefei Huang,Yansu Chen

Acta histochemica 121:695-703 PubMed31239073

2019

Inhibitor of growth 4 (ING4) inhibits hypoxia-induced EMT by decreasing HIF-1α and snail in HK2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lingling Lu,Jing Li,Yuan Le,Hong Jiang

Scientific reports 7:8157 PubMed28811661

2017

Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mercedes Pardo,Lu Yu,Shihpei Shen,Peri Tate,Daniel Bode,Blake L Letney,Dawn E Quelle,William Skarnes,Jyoti S Choudhary

The Prostate 77:49-59 PubMed27527891

2016

Miz1, a Novel Target of ING4, Can Drive Prostate Luminal Epithelial Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Penny L Berger,Mary E Winn,Cindy K Miranti
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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