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AB247702

Anti-ING4 antibody [EP3804] - BSA and Azide free

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Rabbit Recombinant Monoclonal ING4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

My036, ING4, Inhibitor of growth protein 4, p29ING4

1 Images
Western blot - Anti-ING4 antibody [EP3804] - BSA and Azide free (AB247702)
  • WB

Supplier Data

Western blot - Anti-ING4 antibody [EP3804] - BSA and Azide free (AB247702)

This data was developed using ab108621, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ING4 antibody [EP3804] (<a href='/en-us/products/primary-antibodies/ing4-antibody-ep3804-ab108621'>ab108621</a>) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3804

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247702 is the carrier-free version of ab108621.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ING4 also known as Inhibitor of Growth Family Member 4 is a nuclear protein with a molecular mass of approximately 29 kDa. This protein mainly localizes to the nucleus and is expressed in various tissues including the brain and liver. ING4 contains a plant homeodomain (PHD) finger motif which suggests its involvement in chromatin-mediated transcriptional regulation. As a regulator of gene expression ING4 influences cell growth and apoptosis by interacting with histones to affect chromatin structure and function.
Biological function summary

The ING4 protein plays a significant role in tumor suppression. It forms part of a larger protein complex by associating with components like the nucleosome remodeling and histone deacetylase (NuRD) complex. By binding to specific histone modifications ING4 influences transcriptional regulation of several genes that control cell cycle and apoptosis. This activity helps maintain genomic stability and prevent cellular transformation establishing ING4 as a guardian against oncogenic processes.

Pathways

ING4 integrates into cellular machinery that governs the NF-κB signaling pathway and the p53 pathway. These pathways are critical for controlling inflammation and cellular stress responses. Within the NF-κB pathway ING4 interacts with proteins such as IκBα modulating inflammatory cytokine production. In the context of the p53 pathway ING4 acts together with p53 enhancing its tumor suppressor functions through transcriptional activation of p53-responsive genes.

ING4 has a notable association with cancer particularly glioblastoma and breast cancer. Reduced expression or alterations in ING4 have been linked to these malignancies where it functions to suppress the aggressive characteristics of tumor cells. ING4 also connects with proteins like HIF-1α in pathways that regulate cellular responses to hypoxia further implicating its role in tumor biology. Understanding ING4's interactions and effects offers potential for developing therapeutic strategies targeting cancer progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of HBO1 complexes, which specifically mediate acetylation of histone H3 at 'Lys-14' (H3K14ac), and have reduced activity toward histone H4 (PubMed : 16387653). Through chromatin acetylation it may function in DNA replication (PubMed : 16387653). May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation (PubMed : 15251430, PubMed : 15528276). Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA (PubMed : 15029197). May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC (PubMed : 15029197). Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1) (PubMed : 15897452). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity).
See full target information ING4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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