AB255832

Anti-ING5 antibody [7A11] - BSA and Azide free

Recombinant
What is this?

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(2 Publications)

Rat Recombinant Monoclonal ING5 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Inhibitor of growth protein 5, p28ING5, ING5

2 Images

Lab

Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (AB255832)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252838).

All lanes:

Western blot - Anti-ING5 antibody [7A11] (<a href='/en-us/products/primary-antibodies/ing5-antibody-7a11-ab252838'>ab252838</a>) at 0.66 µg/mL

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 3:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 4:

HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 10 µg

Lane 5:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 6:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3s

Lab

Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (AB255832)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252838).

All lanes:

Western blot - Anti-ING5 antibody [7A11] (<a href='/en-us/products/primary-antibodies/ing5-antibody-7a11-ab252838'>ab252838</a>) at 0.66 µg/mL

Lane 1:

Jurkat cytoplasmic fraction at 10 µg

Lane 2:

Jurkat nuclear fraction at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Exposure time: 3s

Unconjugated

Anti-ING5 antibody [7A11]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7A11

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab255832 is the carrier-free version of ab252838.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ING5 also known as Inhibitor of Growth Protein 5 is a protein with a molecular mass of approximately 28 kDa. It is part of the ING family of tumor suppressors and is known for its role in controlling cell growth and apoptosis. This protein is widely expressed in various tissues with notable expression in the brain lungs and liver. ING5 has DNA-binding capabilities and can modulate transcriptional activity affecting several downstream cellular processes.

Biological function summary

ING5 plays a role in regulating cell cycle progression and maintaining genomic stability. It functions within the NuA4 histone acetyltransferase complex influencing chromatin remodeling and gene expression. ING5 interacts with several other proteins like p53 and p300/CBP contributing to its capability to suppress unwanted cellular proliferation. This makes it an important player in maintaining proper cell function and preventing malignant transformation.

Pathways

ING5 interacts with multiple proteins to contribute to the p53 signaling pathway and the DNA damage response pathway. It modulates p53-dependent transcriptional activity enhancing the cell's ability to respond to DNA damage. ING5’s role in these pathways links it to sister proteins in the ING family like ING1 and ING2 which also participate in p53-related responses and cell cycle control.

Altered expression or mutation of ING5 has implications in cancer specifically breast cancer and gastric cancer. Its tumor-suppressive functions are compromised when ING5 is mutated or downregulated leading to increased proliferation and survival of cancerous cells. ING5 is connected to other proteins like p53 and HDAC1 in the context of these cancers highlighting its importance in maintaining normal cellular checks and balances.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the HBO1 complex, which specifically mediates acetylation of histone H3 at 'Lys-14' (H3K14ac) and, to a lower extent, acetylation of histone H4 (PubMed : 24065767). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity (PubMed : 16387653). Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator (PubMed : 12750254, PubMed : 16387653). Inhibits cell growth, induces a delay in S-phase progression and enhances Fas-induced apoptosis in an INCA1-dependent manner (PubMed : 21750715).

See full target information ING5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cartilage :19476035251323601 PubMed40119525

2025

Transcriptional Analysis Reveals That the FHL1/JAK-STAT Pathway is Involved in Acute Cartilage Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Lu,Zhenhua Shi,Lindan Geng,Dong Ren,Haowei Hou,Guowei Ren,Shuangquan Yao,Pengcheng Wang

PloS one 10:e0123736 PubMed25860957

2015

ING5 is phosphorylated by CDK2 and controls cell proliferation independently of p53.

Applications

Unspecified application

Species

Unspecified reactive species

Ulrike Linzen,Richard Lilischkis,Ruwin Pandithage,Britta Schilling,Andrea Ullius,Juliane Lüscher-Firzlaff,Elisabeth Kremmer,Bernhard Lüscher,Jörg Vervoorts

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ING5 antibody [7A11] - BSA and Azide free

Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (AB255832)

Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (AB255832)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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