JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259904

Anti-ING5 antibody [EPR23930-1]

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Recombinant Monoclonal ING5 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 2 publications.

View Alternative Names

Inhibitor of growth protein 5, p28ING5, ING5

9 Images
Flow Cytometry (Intracellular) - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ING5 antibody [EPR23930-1] (AB259904)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling ING5 with ab259904 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ING5 antibody [EPR23930-1] (AB259904)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T cells labelling ING5 with ab259904 at 1/1000 (0.482 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in HEK-293T cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • IP

Supplier Data

Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (AB259904)

ING5 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate with ab259904 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259904 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2 : ab259904 IP in MCF7 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259904 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (ab259904)

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Flow Cytometry (Intracellular) - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ING5 antibody [EPR23930-1] (AB259904)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling ING5 with ab259904 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ING5 antibody [EPR23930-1] (AB259904)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling ING5 with ab259904 at 1/1000 (0.482 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • IP

Supplier Data

Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (AB259904)

ING5 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate with ab259904 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259904 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug

Lane 2 : ab259904 IP in NIH/3T3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259904 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] (ab259904)

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Western blot - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • WB

Lab

Western blot - Anti-ING5 antibody [EPR23930-1] (AB259904)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Lane1-2 : 10 secondsLane3-4 : 15 seconds.

All lanes:

Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Western blot - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • WB

Supplier Data

Western blot - Anti-ING5 antibody [EPR23930-1] (AB259904)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane1-2 : 7.5 seconds; Lane3 : 70 seconds.

All lanes:

Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Western blot - Anti-ING5 antibody [EPR23930-1] (AB259904)
  • WB

Lab

Western blot - Anti-ING5 antibody [EPR23930-1] (AB259904)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure times : Lane1 : 8 seconds; Lane2 : 3 minutes

All lanes:

Western blot - Anti-ING5 antibody [EPR23930-1] (ab259904) at 1/1000 dilution

Lane 1:

His-tagged human ING5 recombinant protein, 20 ng

Lane 2:

His-tagged human ING4 recombinant protein, 20 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

false

  • Carrier free

    Anti-ING5 antibody [EPR23930-1] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23930-1

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB173283

Anti-Carboxypeptidase A antibody [EPR12086]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A antibody [EPR12086] (AB173283)

  • 5
  • 1
Primary Antibodies

AB278081

Anti-COLEC11 antibody [EPR24192-169]

RabMAb|Recombinant

Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

  • 0
  • 0
Primary Antibodies

AB259829

Anti-Ataxin 7 antibody [EPR24136-89]

RabMAb|Recombinant

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (AB259829)

  • 0
  • 0
Primary Antibodies

AB278099

Anti-ROR alpha/RORA antibody [EPR23719-48]

BOND RX™ Validated|RabMAb|Recombinant

Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (AB278099)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ING5 also known as Inhibitor of Growth Protein 5 is a protein with a molecular mass of approximately 28 kDa. It is part of the ING family of tumor suppressors and is known for its role in controlling cell growth and apoptosis. This protein is widely expressed in various tissues with notable expression in the brain lungs and liver. ING5 has DNA-binding capabilities and can modulate transcriptional activity affecting several downstream cellular processes.
Biological function summary

ING5 plays a role in regulating cell cycle progression and maintaining genomic stability. It functions within the NuA4 histone acetyltransferase complex influencing chromatin remodeling and gene expression. ING5 interacts with several other proteins like p53 and p300/CBP contributing to its capability to suppress unwanted cellular proliferation. This makes it an important player in maintaining proper cell function and preventing malignant transformation.

Pathways

ING5 interacts with multiple proteins to contribute to the p53 signaling pathway and the DNA damage response pathway. It modulates p53-dependent transcriptional activity enhancing the cell's ability to respond to DNA damage. ING5’s role in these pathways links it to sister proteins in the ING family like ING1 and ING2 which also participate in p53-related responses and cell cycle control.

Altered expression or mutation of ING5 has implications in cancer specifically breast cancer and gastric cancer. Its tumor-suppressive functions are compromised when ING5 is mutated or downregulated leading to increased proliferation and survival of cancerous cells. ING5 is connected to other proteins like p53 and HDAC1 in the context of these cancers highlighting its importance in maintaining normal cellular checks and balances.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the HBO1 complex, which specifically mediates acetylation of histone H3 at 'Lys-14' (H3K14ac) and, to a lower extent, acetylation of histone H4 (PubMed : 24065767). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity (PubMed : 16387653). Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator (PubMed : 12750254, PubMed : 16387653). Inhibits cell growth, induces a delay in S-phase progression and enhances Fas-induced apoptosis in an INCA1-dependent manner (PubMed : 21750715).
See full target information ING5
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Exploration (Beijing, China) 5:270015 PubMed40585763

2025

The RNA Binding Protein Bcas2 is Required for Antibody Class Switch in Activated-B Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Siyuan Sun,Chenxu Lu,Yixuan Li,Bing Fang,Xiangfeng Tang,Xuepeng Li,Weiru Yu,Yumei Lei,Longjie Sun,Ming Zhang,Jiazeng Sun,Ping Liu,Yongting Luo,Xingwang Zhao,Jing Zhan,Libing Liu,Rong Liu,Jiaqiang Huang,Ziwei Yi,Yifei Yu,Weihan Xiao,Zheng Ding,Lei Li,Dan Su,Fazheng Ren,Changchang Cao,Ran Wang,Wenbiao Shi,Juan Chen

Frontiers in oncology 11:653061 PubMed33996574

2021

Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Danhui Ma,Sinuo Chen,Heming Wang,Jiayi Wei,Hao Wu,Hong Gao,Xinlai Cheng,Taotao Liu,Shi-Hua Luo,Yicheng Zhao,Guangqi Song
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com