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Rabbit Recombinant Monoclonal ING5 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat, Recombinant full length protein - Human samples.

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Images

Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (AB277948), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (AB277948), expandable thumbnail
  • Western blot - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (AB277948), expandable thumbnail
  • Immunoprecipitation - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (AB277948), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ING5 antibody [EPR23930-1] - BSA and Azide free (AB277948), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Expected
Expected
Tested
Not recommended
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Target data

Function

Component of the HBO1 complex, which specifically mediates acetylation of histone H3 at 'Lys-14' (H3K14ac) and, to a lower extent, acetylation of histone H4 (PubMed:24065767). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity (PubMed:16387653). Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator (PubMed:12750254, PubMed:16387653). Inhibits cell growth, induces a delay in S-phase progression and enhances Fas-induced apoptosis in an INCA1-dependent manner (PubMed:21750715).

Alternative names

Inhibitor of growth protein 5, p28ING5, ING5

Recommended products

Rabbit Recombinant Monoclonal ING5 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat, Recombinant full length protein - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR23930-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab277948 is the carrier-free version of Anti-ING5 antibody [EPR23930-1] ab259904.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ING5 also known as Inhibitor of Growth Protein 5 is a protein with a molecular mass of approximately 28 kDa. It is part of the ING family of tumor suppressors and is known for its role in controlling cell growth and apoptosis. This protein is widely expressed in various tissues with notable expression in the brain lungs and liver. ING5 has DNA-binding capabilities and can modulate transcriptional activity affecting several downstream cellular processes.

Biological function summary

ING5 plays a role in regulating cell cycle progression and maintaining genomic stability. It functions within the NuA4 histone acetyltransferase complex influencing chromatin remodeling and gene expression. ING5 interacts with several other proteins like p53 and p300/CBP contributing to its capability to suppress unwanted cellular proliferation. This makes it an important player in maintaining proper cell function and preventing malignant transformation.

Pathways

ING5 interacts with multiple proteins to contribute to the p53 signaling pathway and the DNA damage response pathway. It modulates p53-dependent transcriptional activity enhancing the cell's ability to respond to DNA damage. ING5’s role in these pathways links it to sister proteins in the ING family like ING1 and ING2 which also participate in p53-related responses and cell cycle control.

Associated diseases and disorders

Altered expression or mutation of ING5 has implications in cancer specifically breast cancer and gastric cancer. Its tumor-suppressive functions are compromised when ING5 is mutated or downregulated leading to increased proliferation and survival of cancerous cells. ING5 is connected to other proteins like p53 and HDAC1 in the context of these cancers highlighting its importance in maintaining normal cellular checks and balances.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com