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AB273486

Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free

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Mouse Recombinant Monoclonal Inhibin alpha antibody. Carrier free. Suitable for ELISA, WB, IHC-P and reacts with Human samples.

View Alternative Names

Inhibin alpha chain, INHA

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)

This data was produced using the same clone but in a different formulation, ab273454.

Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 μg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on the theca interna of the human ovary. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)

This data was produced using the same clone but in a different formulation, ab273454.

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 μg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on human testis. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)
  • ELISA

Supplier Data

ELISA - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)

This data was produced using the same clone but in a different formulation, ab273454.

ab273454 used at 0-2000 ng/ml.

Antigen (Human Inhibin alpha) used at 1000 ng/ml.

Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)
  • WB

Supplier Data

Western blot - Anti-Inhibin alpha antibody [PO12/8] - BSA and Azide free (AB273486)

This data was produced using the same clone but in a different formulation, ab273454.

Negative control : Human tonsil.
The molecular weight observed is consistent with what has been described in the literature (PMID : 31658054).

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Western blot - Anti-Inhibin alpha antibody [PO12/8] (<a href='/en-us/products/primary-antibodies/inhibin-alpha-antibody-po12-8-ab273454'>ab273454</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Human tonsil tissue lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PO12/8

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab273486 is the carrier-free version of ab273454.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin alpha also known as alpha-inhibin is a subunit of the inhibin protein complex. As a 32 kDa glycoprotein inhibin exerts its role as a modulator of hormone activities by forming heterodimers with various beta subunits typically expressed in the gonads adrenal glands and pituitary gland. Inhibin alpha not only influences the secretion of follicle-stimulating hormone (FSH) but also acts as an important player in reproductive endocrinology. Its expression can be assessed through inh alpha R1 and po12 markers offering insights through inhibin immunohistochemistry.
Biological function summary

Inhibin alpha forms part of the inhibin hormone complex which primarily serves as a negative regulator of FSH secretion. Insufficient levels of inhibin can disrupt normal metabolic functions impacting reproductive systems. The inhibin alpha subunit is vital for maintaining reproductive homeostasis and monitoring the inhibin staining pattern provides a useful diagnostic tool in reproductive health assessments. Inhibin binds to activin receptors where it blocks FSH release from the anterior pituitary gland reinforcing its role in managing the reproductive axis.

Pathways

Inhibin alpha is critically involved in the transforming growth factor-beta (TGF-beta) signaling pathway. This pathway plays a major role in cell differentiation and proliferation where inhibin competes with activin for the same receptors ultimately reducing FSH synthesis. Additionally inhibin functions within endocrine regulatory mechanisms ensuring the reproductive hormones maintain their balance alongside related proteins such as activin and follistatin each contributing uniquely to signaling processes.

Inhibin alpha alterations can significantly influence conditions like polycystic ovary syndrome (PCOS) and certain gonadal tumors. Abnormal levels of inhibin are often observed in PCOS where disrupted endocrine feedback leads to infertility issues. Gonadal tumors also relate to increased inhibin expression providing a biomarker for diagnosis and progression monitoring. Through these diseases inhibin alpha interacts with proteins such as luteinizing hormone playing part in their systemic dysfunctions within the affected individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.. Inhibin B is a dimer of alpha and beta-B that plays a crucial role in the regulation of the reproductive system by inhibiting the secretion of follicle-stimulating hormone (FSH) from the anterior pituitary gland. Thereby, maintains reproductive homeostasis in both males and females. Acts as a more potent suppressor of FSH release than inhibin A (By similarity). Functions as competitive receptor antagonist binding activin type II receptors with high affinity in the presence of the TGF-beta type III coreceptor/TGFBR3L (PubMed : 34910520).
See full target information INHA
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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