AB242422

Anti-Inhibin alpha antibody [SP323] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Inhibin alpha antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human samples.

View Alternative Names

Inhibin alpha chain, INHA

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224798)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Formalin-fixed, paraffin-embedded human testis tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224798).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224798).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Inhibin alpha using ab224798 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224798).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach adenocarcinoma tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224798)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Inhibin alpha with ab224798 at 1/100 dilution (1.42 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab224798)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP323

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA pH 8.0 buffer before commencing with IHC staining protocol.</p>" } } }

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Product details

ab242422 is the carrier-free version of ab224798.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin alpha also known as alpha-inhibin is a subunit of the inhibin protein complex. As a 32 kDa glycoprotein inhibin exerts its role as a modulator of hormone activities by forming heterodimers with various beta subunits typically expressed in the gonads adrenal glands and pituitary gland. Inhibin alpha not only influences the secretion of follicle-stimulating hormone (FSH) but also acts as an important player in reproductive endocrinology. Its expression can be assessed through inh alpha R1 and po12 markers offering insights through inhibin immunohistochemistry.

Biological function summary

Inhibin alpha forms part of the inhibin hormone complex which primarily serves as a negative regulator of FSH secretion. Insufficient levels of inhibin can disrupt normal metabolic functions impacting reproductive systems. The inhibin alpha subunit is vital for maintaining reproductive homeostasis and monitoring the inhibin staining pattern provides a useful diagnostic tool in reproductive health assessments. Inhibin binds to activin receptors where it blocks FSH release from the anterior pituitary gland reinforcing its role in managing the reproductive axis.

Pathways

Inhibin alpha is critically involved in the transforming growth factor-beta (TGF-beta) signaling pathway. This pathway plays a major role in cell differentiation and proliferation where inhibin competes with activin for the same receptors ultimately reducing FSH synthesis. Additionally inhibin functions within endocrine regulatory mechanisms ensuring the reproductive hormones maintain their balance alongside related proteins such as activin and follistatin each contributing uniquely to signaling processes.

Inhibin alpha alterations can significantly influence conditions like polycystic ovary syndrome (PCOS) and certain gonadal tumors. Abnormal levels of inhibin are often observed in PCOS where disrupted endocrine feedback leads to infertility issues. Gonadal tumors also relate to increased inhibin expression providing a biomarker for diagnosis and progression monitoring. Through these diseases inhibin alpha interacts with proteins such as luteinizing hormone playing part in their systemic dysfunctions within the affected individuals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

See full target information INHA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Inhibin alpha antibody [SP323] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [SP323] - BSA and Azide free (AB242422)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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