AB128958

Anti-Inhibin beta A antibody [EPR2785(2)]

RabMAb
Recombinant
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(11 Publications)

Rabbit Recombinant Monoclonal Inhibin beta A antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 11 publications.

View Alternative Names

Inhibin beta A chain, Activin beta-A chain, Erythroid differentiation protein, EDF, INHBA

3 Images

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Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

All lanes:

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

Mouse brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

Observed band size: 43 kDa

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Unknown

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

All lanes:

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958) at 1/1000 dilution

All lanes:

Human Inhibin beta A recombinant protein at 0.001 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 47 kDa

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OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Inhibin beta A antibody [EPR2785(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2785(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inhibin beta A also known as activin beta A chain and inhibin A is a protein with a significant role in the regulation of diverse physiological processes. This protein has a molecular mass of approximately 45 kDa and originates primarily from gonadal tissues but also from the placenta pituitary gland and other tissues. Inhibin beta A consists of several subunits that can dimerize to form both activins and inhibins depending on the pairing with other subunits like the alpha chain.

Biological function summary

Activin beta A chain plays an important role in the modulation of follicle-stimulating hormone (FSH) regulation cellular growth and differentiation. It often functions as part of a complex with activin and inhibin proteins influencing reproductive hormone levels and participating in tissue repair and inflammation processes. Inhibin beta A forms activin A when paired with another inhibin beta A subunit or inhibin A when paired with an inhibin alpha subunit therefore contributing to the modulation of FSH-releasing protein activity.

Pathways

Activin and inhibin proteins participate in the regulation of the TGF-beta signaling pathway which is essential for reproductive and developmental processes. In this context inhibin beta A interacts functionally with proteins such as follistatin and FSH-releasing protein which modulate pathways that influence follicle maturation and hormone secretion. The protein's role in these pathways highlights its importance in orchestrating hormonal responses and facilitating proper cellular communication within the endocrine system.

Inhibin beta A becomes relevant in the context of reproductive health and oncology. Changes in its expression can associate with disorders like polycystic ovary syndrome (PCOS) and certain cancers particularly ovarian cancer where it may alter hormonal regulation and tumor progression. Related proteins such as inhibin B and follistatin also play roles in these conditions further complicating the pathways and the potential development of therapeutic interventions that target inhibin beta A and its network of interactions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition.. Activin A is a homodimer of INHBA that plays a role in several essential biological processes including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis (PubMed : 3194407, PubMed : 16440334). Signals through type I (such as ACVR1B or ACVR1C) and type II receptors (such as ACVR2A, ACVR2B or BMPR2) which, upon ligand binding, phosphorylate SMAD2 and SMAD3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and modulate gene expression (PubMed : 10652306, PubMed : 24018044). Can also activate alternative non-canonical intracellular signaling pathways including the p38 MAPK, extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases (JNKs) to modulate cell migration and differentiation (PubMed : 16440334). Alternatively, promotes osteoblastic differentiation via ACVRL1-SMAD1/5/9 pathway (PubMed : 34948289). In addition, can engage the type I receptor ACVR1 to form an ACVR1-activin A-type II receptor non-signaling complex (NSC) that renders receptors unavailable for engagement with BMPs, hence resulting in an apparent inhibition of ACVR1-mediated BMP signaling (PubMed : 26333933).. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.

See full target information Inhibin beta A chain

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 26:526 PubMed41013408

2025

INHBA knockdown inhibits renal fibrosis in mice following ischemia-reperfusion injury by suppressing activation of the TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Wang,Qiao Tang,Qian Sun

International endodontic journal 58:991-1005 PubMed40106315

2025

Activin a regulates vascular formation and stabilization in direct coculture of dental pulp stem cells and endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Zhong,Yuchen Zhang,Shulan Lin,Jun Kang,Mingxin Hu,Junqing Liu,Ying Chen,Qianzhou Jiang,Chengfei Zhang

NPJ biofilms and microbiomes 10:39 PubMed38589501

2024

Integrative analysis reveals associations between oral microbiota dysbiosis and host genetic and epigenetic aberrations in oral cavity squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liuyang Cai,Hengyan Zhu,Qianqian Mou,Po Yee Wong,Linlin Lan,Cherrie W K Ng,Pu Lei,Man Kit Cheung,Daijuanru Wang,Eddy W Y Wong,Eric H L Lau,Zenon W C Yeung,Ronald Lai,Katie Meehan,Sherwood Fung,Kwan Chee A Chan,Vivian W Y Lui,Alfred S L Cheng,Jun Yu,Paul K S Chan,Jason Y K Chan,Zigui Chen

Cancer research communications 4:571-587 PubMed38329386

2024

INHBA is Enriched in HPV-negative Oropharyngeal Squamous Cell Carcinoma and Promotes Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tsima Abou Kors,Linda Hofmann,Annika Betzler,Kathrina Payer,Martin Bens,Jens Truong,Adrian von Witzleben,Jaya Thomas,Johann M Kraus,Randa Kalaajieh,Diana Huber,Jasmin Ezić,Julian Benckendorff,Jens Greve,Patrick J Schuler,Christian H Ottensmeier,Hans A Kestler,Thomas K Hoffmann,Marie-Nicole Theodoraki,Cornelia Brunner,Simon Laban

Journal of orthopaedic surgery and research 18:848 PubMed37940978

2023

INHBA gene silencing inhibits proliferation, migration, and invasion of osteosarcoma cells by repressing TGF-β signaling pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Zhang,Yuemei Huang,Qiuting Wen,Yan Li,Lin Guo,Na Ge

Cancers 15: PubMed36612143

2022

Activin B and Activin C Have Opposing Effects on Prostate Cancer Progression and Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Karen L Reader,Simon John-McHaffie,Sylvia Zellhuber-McMillan,Tim Jowett,David G Mottershead,Heather E Cunliffe,Elspeth J Gold

Cell death discovery 8:162 PubMed35379793

2022

GDF-15 in tumor-derived exosomes promotes muscle atrophy via Bcl-2/caspase-3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wanli Zhang,Weikuan Sun,Xiaofan Gu,Chunxiao Miao,Lixing Feng,Qiang Shen,Xuan Liu,Xiongwen Zhang

Translational oncology 18:101333 PubMed35093789

2022

Extracellular vesicles extracted from bone marrow mesenchymal stem cells carrying MicroRNA-342-3p inhibit the INHBA/IL13Rα2 axis to suppress the growth and metastasis of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Liu,Jing Zhang,Yi Liu,Hai Peng,Yingqi Wu

The Journal of international medical research 49:3000605211014998 PubMed34130530

2021

INHBA promotes the proliferation, migration and invasion of colon cancer cells through the upregulation of VCAN.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Guo,Yuan Liu

Molecular medicine reports 23: PubMed33179113

2020

Activation of activin/Smad2 and 3 signaling pathway and the potential involvement of endothelial‑mesenchymal transition in the valvular damage due to rheumatic heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Shenglin Xian,Ang Chen,Xiaodan Wu,Chuanghong Lu,Yunjiao Wu,Feng Huang,Zhiyu Zeng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Inhibin beta A antibody [EPR2785(2)]

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] (AB128958)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise