Anti-Inhibin beta E chain antibody

• WB

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Western blot - Anti-Inhibin beta E chain antibody (AB103167)

All lanes:

Western blot - Anti-Inhibin beta E chain antibody (ab103167) at 1/500 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 39 kDa

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• WB

Unknown

Western blot - Anti-Inhibin beta E chain antibody (AB103167)

All lanes:

Western blot - Anti-Inhibin beta E chain antibody (ab103167) at 1/500 dilution

All lanes:

Human liver tissue lysate at 50 µg

Predicted band size: 118 kDa,39 kDa,49 kDa,67 kDa

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• WB

Lab

Western blot - Anti-Inhibin beta E chain antibody (AB103167)

Left : Anti-Inhibin beta E chain antibody ab103167 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

Right : Rabbit Monoclonal [EPR29191-125] to Inhibin beta E chain ab322202 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 43 kDa in INHBE-DDDDK HEK-293T overexpression lysate with no signal observed at this size in INHBC-DDDDK HEK-293T overexpression lysate.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution. For DDDDK-tag detection, HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) was used at 1/1000 dilution and developed using the ECL technique.

Neither ab103167 nor ab322202 cross-reacts with Inhibin beta C chain (INHBC). In Western Blot, we recommend ab322202 as the superior alternative to ab103167 for better specificity and sensitivity.

Exposure time for DDDDK tag : 2s.

Lanes 1 - 4:

Western blot - Anti-Inhibin beta E chain antibody (ab103167) at 1/1000 dilution

Lanes 2, 4, 6 and 8:

Western blot - HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (<a href='/en-us/products/primary-antibodies/hrp-ddddk-tag-binds-to-flag-tag-sequence-antibody-m2-ab49763'>ab49763</a>)

Lanes 5 - 8:

Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (<a href='/en-us/products/primary-antibodies/inhibin-beta-e-chain-antibody-epr29191-125-ab322202'>ab322202</a>) at 1/1000 dilution

Lanes 1, 3, 5 and 7:

HEK-293 Mock Transfection Lysate at 20 µg

Lanes 2 and 6:

INHBE-DDDDK HEK-293T Overexpression Lysate at 20 µg

Lanes 4 and 8:

INHBC-DDDDK HEK-293T Overexpression Lysate at 20 µg

Secondary

Lanes 1 - 8:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 8:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 35 kDa

Observed band size: 43 kDa

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• WB

Unknown

Western blot - Anti-Inhibin beta E chain antibody (AB103167)

All lanes:

Western blot - Anti-Inhibin beta E chain antibody (ab103167) at 1/500 dilution

Lane 1:

INHBE transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 23 kDa,29 kDa,31 kDa,32 kDa,33 kDa,39 kDa,41 kDa,43 kDa,48 kDa,59 kDa,60 kDa,69 kDa,96 kDa

Observed band size: 35 kDa,60 kDa

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