Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)

Rabbit Recombinant Monoclonal Inhibin beta E chain antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (AB322202), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P
Human	Tested	Tested
Mouse	Tested	Tested
Recombinant fragment - Human	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information INHBE

Function

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Alternative names

Inhibin beta E chain, Activin beta-E chain, INHBE

Recommended products

Rabbit Recombinant Monoclonal Inhibin beta E chain antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29191-125
Purification technique: Affinity purification Protein A
Specificity: Not suitable for WB in cell lines.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Inhibin beta E chain also known as 'INHBE' is a protein with multiple roles in the human body weighing about 42 kDa. It is a member of the transforming growth factor-beta (TGF-β) superfamily. The protein can be expressed in many tissues including the liver and placenta. INHBE is associated with other proteins to form different complexes affecting various biological processes.

Biological function summary

The Inhibin beta E chain participates in the regulation of reproductive hormone activity. It forms either homodimers or heterodimers combining with other inhibin and activin molecules. The dimerization with alpha chains forms inhibins while pairing with other beta subunits forms activins. These complexes then modulate signaling pathways that adjust hormonal secretion particularly in the pituitary and gonadal tissues.

Pathways

Inhibin beta E chain plays a significant role in the activin signaling pathway. This pathway affects the regulation of FSH (follicle-stimulating hormone) secretion which is important for reproductive processes. INHBE relates to proteins such as follistatin and activin A within these pathways influencing activities flowing from ligand-receptor interactions to transcriptional regulation.

Associated diseases and disorders

Inhibin beta E chain has been linked to certain reproductive and metabolic disorders. For instance abnormal expression or dysfunction of INHBE may relate to polycystic ovary syndrome (PCOS) affecting women’s fertility. Liver diseases such as non-alcoholic fatty liver disease (NAFLD) also show associations with INHBE levels. In these contexts INHBE interacts with various TGF-β family proteins contributing to either disease progression or regulation.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human Inhibin beta C chain.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202) at 1/1000 dilution
Lane 1: His-tagged human Inhibin beta E chain fragment at 10 ng
Lane 2: His-tagged human Inhibin beta C chain fragment at 10 ng
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 15 kDa
Exposure time: 180s
Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle, spleen, ovary, uterus (PMID: 12242034, PMID: 11932210, PMID: 9070865).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse skeletal muscle tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse ovary tissue lysate at 20 µg
Lane 5: Mouse uterus tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa
Exposure time: 180s
Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: skeletal muscle, uterus (PMID: 12242034, PMID: 11932210, PMID: 9070865).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 35999217, PMID: 35896531).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202) at 1/1000 dilution
Lane 1: Human liver tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
Lane 3: Human uterus tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 48 kDa, 36 kDa
Exposure time: 180s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Inhibin beta E chain with ab322202 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse spleen.

The section was incubated with ab322202 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Inhibin beta E chain with ab322202 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human skeletal muscle.

The section was incubated with ab322202 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling Inhibin beta E chain with ab322202 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in mouse skeletal muscle.

The section was incubated with ab322202 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Inhibin beta E chain with ab322202 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human liver.

The section was incubated with ab322202 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Inhibin beta E chain with ab322202 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in mouse liver.

The section was incubated with ab322202 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin beta E chain antibody [EPR29191-125] (ab322202)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Inhibin beta E chain with ab322202 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human spleen.

The section was incubated with ab322202 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins