Rabbit Polyclonal INO80 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human INO80 aa 1500 to C-terminus.
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- Scientific reports 13:132352023Chromatin remodeling factor, INO80, inhibits PMAIP1 in renal tubular cells via exchange of histone variant H2A.Z. for H2A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRika Miura,Imari Mimura,Hanako Saigusa,Tomotaka Yamazaki,Fumiaki Tanemoto,Yu Kurata,Dai Sato,Tetsuhiro Tanaka,Masaomi NangakuPubMed 37580530
- Cell reports 37:1101382021IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa-Lee Ka,Ga Young Lim,Sewon Hwang,Seung-Su Kim,Mi-Ock LeePubMed 34936865
- Genome biology 22:2942021The chromatin remodeler Ino80 mediates RNAPII pausing site determination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoungseo Cheon,Sungwook Han,Taemook Kim,Daehee Hwang,Daeyoup LeePubMed 34663418
- The EMBO journal 39:e1050982020Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristian Schutt et. al.PubMed 32960481
- Nature communications 11:45342020Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa Prendergast et. al.PubMed 32913330
- Cells 9:2020VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Moreno-Andrés et. al.PubMed 32708675
- The Biochemical journal 476:3053-30662019BAP1 promotes stalled fork restart and cell survival via INO80 in response to replication stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan-Sae Lee,Hye-Ran Seo,Shin-Ai Lee,Soohee Choi,Dongmin Kang,Jongbum KwonPubMed 31657441
- Oncotarget 8:115041-1150532017INO80 haploinsufficiency inhibits colon cancer tumorigenesis via replication stress-induced apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShin-Ai Lee et. al.PubMed 29383140
- Nature communications 8:155402017Structure of the Rpn13-Rpn2 complex provides insights for Rpn13 and Uch37 as anticancer targets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuxiu Lu et. al.PubMed 28598414
- The Journal of biological chemistry 292:9320-93342017Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian H Lee et. al.PubMed 28381557
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human INO80 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULG1
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Bat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Bat | Dilution info - | Notes - |
Associated Products
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Target data
Function
ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair (PubMed:16230350, PubMed:16298340, PubMed:17721549, PubMed:20237820, PubMed:20855601). Binds DNA (PubMed:16298340, PubMed:21303910). As part of the INO80 complex, remodels chromatin by shifting nucleosomes (PubMed:16230350, PubMed:21303910). Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator (PubMed:17721549). Involved in UV-damage excision DNA repair (PubMed:20855601). The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation (PubMed:20687897). Involved in DNA replication (PubMed:20237820). Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle (PubMed:20237820).
Alternative names
INO80A, INOC1, KIAA1259, INO80, Chromatin-remodeling ATPase INO80, hINO80, DNA helicase-related INO80 complex homolog 1, DNA helicase-related protein INO80, INO80 complex subunit A
Recommended products
Rabbit Polyclonal INO80 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human INO80 aa 1500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human INO80 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9ULG1
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab118787 was affinity purified using an epitope specific to INO80 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
INO80 also known as INO80 complex subunit or INO80 ATPase is a chromatin remodeling enzyme weighing approximately 180 kDa. It is located within the nucleus of cells and is found across various tissues. INO80 plays an important mechanical role in modifying the structure of chromatin by sliding and ejecting nucleosomes enabling DNA accessibility for replication repair recombination and transcription processes.
- Biological function summary
The INO80 complex affects gene expression and DNA stability. The INO80 protein forms part of the larger INO80 chromatin remodeling complex which includes several other subunits. This complex helps regulate chromatin structure by repositioning nucleosomes and altering histone-DNA interactions. These actions support the maintenance of genome integrity and facilitate accurate gene expression by modifying the accessibility of DNA to transcription factors and the transcription machinery.
- Pathways
The INO80 complex impacts critical pathways for DNA repair and replication such as the homologous recombination repair pathway and the cell cycle checkpoint pathways. The homologous recombination repair is essential for fixing double-strand breaks in DNA and involves related proteins such as RAD51. Moreover INO80 interacts with proteins like RuvB-like helicases which assist in the structural rearrangement of chromatin during these processes.
- Associated diseases and disorders
Malfunction of the INO80 complex relates to cancer and neurodegenerative diseases. Aberrant functions of INO80 can lead to genomic instability and compromised DNA repair mechanisms contributing to tumorigenesis. In cancer its interaction with proteins such as ATR influences cellular responses to DNA damage. Additionally disruptions in the normal functioning of chromatin remodeling proteins including INO80 have implications in neurodegenerative diseases where INO80’s role in maintaining chromatin structure can impact neuronal survival and health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-INO80 antibody (ab118787)
All lanes: Western blot - Anti-INO80 antibody (ab118787) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 176 kDa
Exposure time: 30s
Immunoprecipitation - Anti-INO80 antibody (ab118787)
Detection of INO80 in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118787 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-INO80 antibody (ab118787)
Predicted band size: 176 kDa
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