AB118787

Anti-INO80 antibody

Name: Anti-INO80 antibody (ab118787) | Abcam
Brand: Abcam Limited
SKU: AB118787
Rating: 4 (1 reviews)

(1 Review)

(16 Publications)

Rabbit Polyclonal INO80 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human INO80 aa 1500 to C-terminus.

View Alternative Names

INO80A, INOC1, KIAA1259, INO80, Chromatin-remodeling ATPase INO80, hINO80, DNA helicase-related INO80 complex homolog 1, DNA helicase-related protein INO80, INO80 complex subunit A

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Immunoprecipitation - Anti-INO80 antibody (AB118787)

Detection of INO80 in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118787 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-INO80 antibody (ab118787)

Predicted band size: 176 kDa

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Western blot - Anti-INO80 antibody (AB118787)

All lanes:

Western blot - Anti-INO80 antibody (ab118787) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 176 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human INO80 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9ULG1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab118787 was affinity purified using an epitope specific to INO80 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INO80 also known as INO80 complex subunit or INO80 ATPase is a chromatin remodeling enzyme weighing approximately 180 kDa. It is located within the nucleus of cells and is found across various tissues. INO80 plays an important mechanical role in modifying the structure of chromatin by sliding and ejecting nucleosomes enabling DNA accessibility for replication repair recombination and transcription processes.

Biological function summary

The INO80 complex affects gene expression and DNA stability. The INO80 protein forms part of the larger INO80 chromatin remodeling complex which includes several other subunits. This complex helps regulate chromatin structure by repositioning nucleosomes and altering histone-DNA interactions. These actions support the maintenance of genome integrity and facilitate accurate gene expression by modifying the accessibility of DNA to transcription factors and the transcription machinery.

Pathways

The INO80 complex impacts critical pathways for DNA repair and replication such as the homologous recombination repair pathway and the cell cycle checkpoint pathways. The homologous recombination repair is essential for fixing double-strand breaks in DNA and involves related proteins such as RAD51. Moreover INO80 interacts with proteins like RuvB-like helicases which assist in the structural rearrangement of chromatin during these processes.

Malfunction of the INO80 complex relates to cancer and neurodegenerative diseases. Aberrant functions of INO80 can lead to genomic instability and compromised DNA repair mechanisms contributing to tumorigenesis. In cancer its interaction with proteins such as ATR influences cellular responses to DNA damage. Additionally disruptions in the normal functioning of chromatin remodeling proteins including INO80 have implications in neurodegenerative diseases where INO80’s role in maintaining chromatin structure can impact neuronal survival and health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair (PubMed : 16230350, PubMed : 16298340, PubMed : 17721549, PubMed : 20237820, PubMed : 20855601). Binds DNA (PubMed : 16298340, PubMed : 21303910). As part of the INO80 complex, remodels chromatin by shifting nucleosomes (PubMed : 16230350, PubMed : 21303910). Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator (PubMed : 17721549). Involved in UV-damage excision DNA repair (PubMed : 20855601). The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation (PubMed : 20687897). Involved in DNA replication (PubMed : 20237820). Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle (PubMed : 20237820).

See full target information INO80

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Glia 73:1307-1323 PubMed40017313

2025

The INO80 Chromatin Remodeling Complex Regulates Histone H2A.Z Mobility and the G1-S Transition in Oligodendrocyte Precursors.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan L Wright,Yi Jiang,Stuart G Nayar,Huiliang Li,William D Richardson

Molecular cell 84:2525-2541.e12 PubMed38906142

2024

Basis of gene-specific transcription regulation by the Integrator complex.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Sabath,Amena Nabih,Christian Arnold,Rim Moussa,David Domjan,Judith B Zaugg,Stefanie Jonas

Nature 627:671-679 PubMed38448585

2024

Decoding chromatin states by proteomic profiling of nucleosome readers.

Applications

Unspecified application

Species

Unspecified reactive species

Saulius Lukauskas,Andrey Tvardovskiy,Nhuong V Nguyen,Mara Stadler,Peter Faull,Tina Ravnsborg,Bihter Özdemir Aygenli,Scarlett Dornauer,Helen Flynn,Rik G H Lindeboom,Teresa K Barth,Kevin Brockers,Stefanie M Hauck,Michiel Vermeulen,Ambrosius P Snijders,Christian L Müller,Peter A DiMaggio,Ole N Jensen,Robert Schneider,Till Bartke

Scientific reports 13:13235 PubMed37580530

2023

Chromatin remodeling factor, INO80, inhibits PMAIP1 in renal tubular cells via exchange of histone variant H2A.Z. for H2A.

Applications

Unspecified application

Species

Unspecified reactive species

Rika Miura,Imari Mimura,Hanako Saigusa,Tomotaka Yamazaki,Fumiaki Tanemoto,Yu Kurata,Dai Sato,Tetsuhiro Tanaka,Masaomi Nangaku

Cell reports 37:110138 PubMed34936865

2021

IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Na-Lee Ka,Ga Young Lim,Sewon Hwang,Seung-Su Kim,Mi-Ock Lee

Genome biology 22:294 PubMed34663418

2021

The chromatin remodeler Ino80 mediates RNAPII pausing site determination.

Applications

Unspecified application

Species

Unspecified reactive species

Youngseo Cheon,Sungwook Han,Taemook Kim,Daehee Hwang,Daeyoup Lee

The EMBO journal 39:e105098 PubMed32960481

2020

Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Schutt,Alix Hallmann,Salma Hachim,Ina Klockner,Melissa Valussi,Ann Atzberger,Johannes Graumann,Thomas Braun,Thomas Boettger

Nature communications 11:4534 PubMed32913330

2020

Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Prendergast,Urszula L McClurg,Rossitsa Hristova,Rolando Berlinguer-Palmini,Sarah Greener,Katie Veitch,Inmaculada Hernandez,Philippe Pasero,Daniel Rico,Jonathan M G Higgins,Anastas Gospodinov,Manolis Papamichos-Chronakis

Cells 9: PubMed32708675

2020

VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Moreno-Andrés,Hideki Yokoyama,Anja Scheufen,Guillaume Holzer,Hongqi Lue,Anna Katharina Schellhaus,Marion Weberruss,Masatoshi Takagi,Wolfram Antonin

The Biochemical journal 476:3053-3066 PubMed31657441

2019

BAP1 promotes stalled fork restart and cell survival via INO80 in response to replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Sae Lee,Hye-Ran Seo,Shin-Ai Lee,Soohee Choi,Dongmin Kang,Jongbum Kwon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-INO80 antibody

Immunoprecipitation - Anti-INO80 antibody (AB118787)

Western blot - Anti-INO80 antibody (AB118787)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

Product promise