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AB245619

Anti-INO80 antibody

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(1 Publication)

Rabbit Polyclonal INO80 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human INO80 aa 1400-1450.

View Alternative Names

INO80A, INOC1, KIAA1259, INO80, Chromatin-remodeling ATPase INO80, hINO80, DNA helicase-related INO80 complex homolog 1, DNA helicase-related protein INO80, INO80 complex subunit A

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Immunoprecipitation - Anti-INO80 antibody (AB245619)
  • IP

Supplier Data

Immunoprecipitation - Anti-INO80 antibody (AB245619)

INO80 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245619 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using another anti-INO80 antibody at 1 μg/ml.

Lane 1 : ab245619 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-INO80 antibody (ab245619)

Predicted band size: 176 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human INO80 aa 1400-1450. The exact immunogen used to generate this antibody is proprietary information.

Q9ULG1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245619 was affinity purified using an epitope specific to INO80 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INO80 also known as INO80 complex subunit or INO80 ATPase is a chromatin remodeling enzyme weighing approximately 180 kDa. It is located within the nucleus of cells and is found across various tissues. INO80 plays an important mechanical role in modifying the structure of chromatin by sliding and ejecting nucleosomes enabling DNA accessibility for replication repair recombination and transcription processes.
Biological function summary

The INO80 complex affects gene expression and DNA stability. The INO80 protein forms part of the larger INO80 chromatin remodeling complex which includes several other subunits. This complex helps regulate chromatin structure by repositioning nucleosomes and altering histone-DNA interactions. These actions support the maintenance of genome integrity and facilitate accurate gene expression by modifying the accessibility of DNA to transcription factors and the transcription machinery.

Pathways

The INO80 complex impacts critical pathways for DNA repair and replication such as the homologous recombination repair pathway and the cell cycle checkpoint pathways. The homologous recombination repair is essential for fixing double-strand breaks in DNA and involves related proteins such as RAD51. Moreover INO80 interacts with proteins like RuvB-like helicases which assist in the structural rearrangement of chromatin during these processes.

Malfunction of the INO80 complex relates to cancer and neurodegenerative diseases. Aberrant functions of INO80 can lead to genomic instability and compromised DNA repair mechanisms contributing to tumorigenesis. In cancer its interaction with proteins such as ATR influences cellular responses to DNA damage. Additionally disruptions in the normal functioning of chromatin remodeling proteins including INO80 have implications in neurodegenerative diseases where INO80’s role in maintaining chromatin structure can impact neuronal survival and health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair (PubMed : 16230350, PubMed : 16298340, PubMed : 17721549, PubMed : 20237820, PubMed : 20855601). Binds DNA (PubMed : 16298340, PubMed : 21303910). As part of the INO80 complex, remodels chromatin by shifting nucleosomes (PubMed : 16230350, PubMed : 21303910). Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator (PubMed : 17721549). Involved in UV-damage excision DNA repair (PubMed : 20855601). The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation (PubMed : 20687897). Involved in DNA replication (PubMed : 20237820). Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle (PubMed : 20237820).
See full target information INO80
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxicology research 13:tfae096 PubMed38957783

2024

LncRNA HILPDA promotes contrast-induced acute kidney injury by recruiting eIF4B to upregulate XPO1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wei,Tuersun Tilakezi,Wei Feng,Heyin Yang,Shujun Yang
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Product promise

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