Anti-iNOS antibody is a rabbit polyclonal antibody that is used to detect iNOS in IHC-P, Western blot. Suitable for Mouse, Rat, Recombinant fragment samples.
- Cited in over 595 publications
- Tried and trusted by researchers since 2004
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- Frontiers in immunology 14:12777452023Pathological granuloma fibrosis induced by agar-embedded in C57BL/6JNarl mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiu-Ju Yang,Chih-Hao Hsu,Chi-Yun Lai,Pei-Chu Tsai,Yung-Deng Song,Chang-Ching Yeh,Yih-Yuan Chen,Horng-Yunn DouPubMed 38146374
- Nature communications 14:77832023Local H release remodels senescence microenvironment for improved repair of injured bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengqiang Chen,Yuanman Yu,Songqing Xie,Danna Liang,Wei Shi,Sizhen Chen,Guanglin Li,Wei Tang,Changsheng Liu,Qianjun HePubMed 38012166
- Aging 15:11740-1176320231,5-anhydro-D-fructose induces anti-aging effects on aging-associated brain diseases by increasing 5'-adenosine monophosphate-activated protein kinase activity via the peroxisome proliferator-activated receptor-γ co-activator-1α/brain-derived neurotrophic factor pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiyoshi Kikuchi,Shotaro Otsuka,Seiya Takada,Kazuki Nakanishi,Kentaro Setoyama,Harutoshi Sakakima,Eiichiro Tanaka,Ikuro MaruyamaPubMed 37950725
- Frontiers in immunology 14:12644472023SARS-CoV-2 N protein induced acute kidney injury in diabetic db/db mice is associated with a Mincle-dependent M1 macrophage activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Wu,Wenbiao Wang,Liying Liang,Junzhe Chen,Sifan Sun,Biao Wei,Yu Zhong,Xiao-Ru Huang,Jian Liu,Xiaoqin Wang,Xueqing Yu,Hui-Yao LanPubMed 38022581
- Bioengineering (Basel, Switzerland) 10:20233D-Printed Medical-Grade Polycaprolactone (mPCL) Scaffold for the Surgical Treatment of Vaginal Prolapse and Abdominal Hernias.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMairim Russo Serafini,Alexandra Mowat,Susanah Mustafa,Siamak Saifzadeh,Tara Shabab,Onur Bas,Nicholas O'Rourke,Dietmar W Hutmacher,Flavia Medeiros SaviPubMed 38002366
- Molecular neurobiology 61:2357-23662023Electroacupuncture Ameliorates Neuronal Injury by NLRP3/ASC/Caspase-1 Mediated Pyroptosis in Cerebral Ischemia-Reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Tang,Yan Li,Xifa Xu,Guangzhong Du,Huanyuan WangPubMed 37874480
- Journal of experimental & clinical cancer research : CR 42:2702023UGDH promotes tumor-initiating cells and a fibroinflammatory tumor microenvironment in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrittney S Harrington,Rahul Kamdar,Franklin Ning,Soumya Korrapati,Michael W Caminear,Lidia F Hernandez,Donna Butcher,Elijah F Edmondson,Nadia Traficante,Joy Hendley,Madeline Gough,Rebecca Rogers,Rohan Lourie,Jyoti Shetty,Bao Tran,Fathi Elloumi,Abdalla Abdelmaksoud,Madhu Lal Nag,Krystyna Mazan-Mamczarz,Carrie D House,John D Hooper,Christina M AnnunziataPubMed 37858159
- PeerJ 11:e160522023Molecular and phenotypic distinctions of macrophages in tolerant and susceptible to hypoxia rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDzhuliia Dzhalilova,Anna Kosyreva,Anastasiya Lokhonina,Ivan Tsvetkov,Polina Vishnyakova,Olga Makarova,Timur FatkhudinovPubMed 37842051
- Stem cell reviews and reports 19:2869-28852023The Lower in Vivo Osteogenicity of Adipose Tissue-Derived Stem Cells Correlates with a Higher Innate Immune Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManon Maroquenne,Marianne Bourguignon,Nathanael Larochette,Hanane El-Hafci,Morgane Margottin,Esther Potier,Delphine Logeart-AvramoglouPubMed 37642900
- Advanced healthcare materials 12:e23015612023A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiu-Gen Li,Xiao-Xin Yang,Hua-Zhen Xu,Ting-Ting Yu,Qi-Rui Li,Jun Hu,Xing-Chun Peng,Ning Han,Xiang Xu,Nan-Nan Chen,Xiao Chen,Jun-Ming Tang,Tong-Fei LiPubMed 37567571
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Tested | Expected |
| Recombinant full length protein - Mouse | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes Please use at a assay dependent concentration. 1μg/ml was used of a 0.2mg/ml stock (lot GR126616-1). Otherwise 1/250 is recommended for any batches at lower concentration. |
Species Mouse | Dilution info - | Notes Please use at a assay dependent concentration. 1μg/ml was used of a 0.2mg/ml stock (lot GR126616-1). Otherwise 1/250 is recommended for any batches at lower concentration. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Alternative names
NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS
Recommended products
Anti-iNOS antibody is a rabbit polyclonal antibody that is used to detect iNOS in IHC-P, Western blot. Suitable for Mouse, Rat, Recombinant fragment samples.
- Cited in over 595 publications
- Tried and trusted by researchers since 2004
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
The immunogen used to raise this antibody shares 84% homology with Rat iNOS.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-iNOS antibody (ab15323) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in mouse, recombinant fragment samples.
Anti-iNOS antibody (ab15323) specifically detects iNOS (UniProt ID: P29477; Molecular weight: 131kDa) and is sold in 500 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-iNOS antibody (ab15323) has high sensitivity and specificity.
Anti-iNOS antibody (ab15323) has been cited over 595 times in peer reviewed journals and is trusted by the scientific community.
Anti-iNOS antibody (ab15323) has 20 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
- Biological function summary
INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.
- Pathways
INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.
- Associated diseases and disorders
INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-iNOS antibody (ab15323)
This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system under denaturing, reducing conditions. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with rabbit polyclonal to iNOS (ab15323; 1 ug x mL-1 (based on antibody concentration of 0.2mg/ml - lot GR126616-1) and the loading control mouse anti-GAPDH antibody (Anti-GAPDH antibody [6C5] - Loading Control ab8245; 1:10000) overnight at 4°C. Antibody binding was detected using infrared (IR) labelled goat anti-rabbit (green; 1:10000) and IR-goat anti-mouse (red; 1:10000) for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-iNOS antibody (ab15323) at 1 µg/mL
Lane 1: Raw264.7 at 20 µg
Lane 2: Raw264.7 + LPS (1 ug per mL; 18 hours) at 20 µg
SecondaryAll lanes: IR- goat anti-rabbit (green) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Western blot - Anti-iNOS antibody (ab15323)
All lanes: Western blot - Anti-iNOS antibody (ab15323) at 5 µg/mL
Lane 1: Raw 264.7 whole cell lysate + PMA at 20 µg
Lane 2: Raw 264.7 whole cell lysate + PMA + LPS + Brefeldin A at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa
Exposure time: 8min
Western blot - Anti-iNOS antibody (ab15323)
Membrane was blocked in 2% milk for 1 hour at RT. Primary anitbody was incubated at 4°C overnight.
All lanes: Western blot - Anti-iNOS antibody (ab15323) at 5 µg/mL
Lane 1: Human recombinant iNOS protein at 0.1 µg
Lane 2: Mouse recombinant iNOS protein at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 131 kDa, 47 kDa, 68 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab15323)
IHC image of iNOS staining in a section of formalin-fixed paraffin-embedded normal mouse lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15323, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab15323)
IHC image of iNOS staining in a section of formalin-fixed paraffin-embedded normal rat lung performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15323, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab15323)
Immunohistochemical analysis of formalin fixed paraffin embedded mouse lung labelling iNOS with ab15323 at a concentration of 1µg/ml.
The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a ChromoMap DAB kit and anti-rabbit HQ and anti-HQ HRP detection.
Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab15323 Anti-iNOS antibody was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
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