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Rabbit Recombinant Monoclonal iNOS antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NOS2 aa 1100 to C-terminus.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human NOS2 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35228
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).

Alternative names

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Rabbit Recombinant Monoclonal iNOS antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NOS2 aa 1100 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human NOS2 aa 1100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P35228
Clone number

BLR188J

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Supplementary info

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Activity summary

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

Associated diseases and disorders

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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2 product images

  • Western blot - Anti-iNOS antibody [BLR188J] - BSA free (ab314088), expandable thumbnail

    Western blot - Anti-iNOS antibody [BLR188J] - BSA free (ab314088)

    Samples: Whole cell lysate (10 µg) from DLD-1 and DLD-1 induced (IFNgamma (56 ng/mL), IL-1beta (145 pg/mL), and TNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibody: Rabbit anti-iNOS recombinant monoclonal antibody [BLR188J] (A700-188CF) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

    All lanes: Western blot - Anti-iNOS antibody [BLR188J] - BSA free (AB314088) at 1/1000 dilution

    Lane 1: DLD-1 Whole cell lysate at 10 µg

    Lane 2: DLD-1 induced Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-iNOS antibody [BLR188J] - BSA free (ab314088), expandable thumbnail

    Immunoprecipitation - Anti-iNOS antibody [BLR188J] - BSA free (ab314088)

    Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from induced DLD-1 cells (hIFN-gamma (56 ng/mL), hIL-1beta (145 pg/mL), and hTNFa (10 ng/mL)) prepared using NETN lysis buffer. Antibodies: Rabbit anti-iNOS recombinant monoclonal antibody [BLR188J] (A700-188) used for IP at 20 µl/mg lysate. iNOS was also immunoprecipitated by a second antibody against a different epitope of iNOS (BL-2053B-2F8). For blotting immunoprecipitated iNOS, A700-188 was used at 1:1000. Chemiluminescence with an exposure time of 30 seconds.

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Product protocols

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