AB205529

Anti-iNOS antibody [EPR16630]

Name: Anti-iNOS antibody [EPR16630] (ab205529) | Abcam
Brand: Abcam Limited
SKU: AB205529
Price: 355 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal iNOS antibody. Suitable for IP, ELISA, WB and reacts with Mouse, Rat, Recombinant fragment - Mouse samples. Cited in 6 publications.

View Alternative Names

Inosl, Nos2, Inducible NO synthase, Macrophage NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Inducible NOS, iNOS, MAC-NOS

5 Images

Supplier Data

Immunoprecipitation - Anti-iNOS antibody [EPR16630] (AB205529)

iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell extract with ab205529 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab205529 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract 10ug (Input).

Lane 2 : ab205529 IP in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab205529 in RAW 264.7 treated with 0.1 mg/ml LPS for 6 hours whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds.

All lanes:

Immunoprecipitation - Anti-iNOS antibody [EPR16630] (ab205529)

Predicted band size: 131 kDa

false

ELISA - Anti-iNOS antibody [EPR16630] (AB205529)

ELISA

Supplier Data

ELISA - Anti-iNOS antibody [EPR16630] (AB205529)

ELISA analysis of House mouse iNOS recombinant protein at 1000 ng/mL with ab205529. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Supplier Data

Western blot - Anti-iNOS antibody [EPR16630] (AB205529)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution

Lane 1:

Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 µg/ml LPS for 6 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 131 kDa

Observed band size: 131 kDa

true

Exposure time: 1s

Supplier Data

Western blot - Anti-iNOS antibody [EPR16630] (AB205529)

The molecular weight observed and the treatment method are consistent with what has been described in the literature (PMID : 12062366)

All lanes:

Western blot - Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution

Lane 1:

Untreated L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Lane 2:

L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

false

Lab

Western blot - Anti-iNOS antibody [EPR16630] (AB205529)

Blocking and dilution buffer : 5% NFDM/TBST iNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID : 11138926, PMID : 16156895, PMID : 10322315).

All lanes:

Western blot - Anti-iNOS antibody [EPR16630] (ab205529) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse colon cancer tissue lysate at 20 µg

Observed band size: 131 kDa

false

Carrier free

Anti-iNOS antibody [EPR16630] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16630

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, ELISA, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We were unable to detect a positive signal in inflammation‑induced THP‑1 cells. According to published studies (PMID:33553532, PMID: 24477906), human macrophages produce very little detectable iNOS and NO in response to inflammatory stimuli due to epigenetic regulation around the NOS2 transcription start site.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/80", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant fragment - Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed : 16373578). As component of the iNOS-S100A8/9 transnitrosylase complex is involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (By similarity).

See full target information Nos2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 38:e70062 PubMed39575578

2024

Mincle Maintains M1 Polarization of Macrophages and Contributes to Renal Aging Through the Syk/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshuang Sun,Hua Liu,Kehui Shi,Meng Wei,Hongli Jiang

Journal of radiation research 65:291-302 PubMed38588586

2024

Hydrogen-rich solution alleviates acute radiation pneumonitis by regulating oxidative stress and macrophages polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yin,Wenjing Xu,Junjun Ling,Lihai Ma,Hao Zhang,Pei Wang

Obesity facts 17:24-36 PubMed37820603

2023

Ube2L6 Promotes M1 Macrophage Polarization in High-Fat Diet-Fed Obese Mice via ISGylation of STAT1 to Trigger STAT1 Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqian Li,Xiao Dong,Wenqian He,Huibiao Quan,Kaining Chen,Chaoping Cen,Weiping Wei

Journal of healthcare engineering 2022:1890892 PubMed35368928

2022

Tetramethylpyrazine Improves Monocrotaline-Induced Pulmonary Hypertension through the ROS/iNOS/PKG-1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Peng Yang,Wen-Peng Dong,Yong-Chao Yang,Yuan-Yuan Zeng,Ying Liu,Zhu Dong,Xi-Miao Ma,Yi-Qiu Cao,Yi-Zhou Bai,Bo Yang,Xiao-Wu Wang

Cell metabolism 34:533-548.e12 PubMed35305295

2022

Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Rosina,Veronica Ceci,Riccardo Turchi,Li Chuan,Nicholas Borcherding,Francesca Sciarretta,María Sánchez-Díaz,Flavia Tortolici,Keaton Karlinsey,Valerio Chiurchiù,Claudia Fuoco,Rocky Giwa,Rachael L Field,Matteo Audano,Simona Arena,Alessandro Palma,Federica Riccio,Farnaz Shamsi,Giovanni Renzone,Martina Verri,Anna Crescenzi,Salvatore Rizza,Fiorella Faienza,Giuseppe Filomeni,Sander Kooijman,Stefano Rufini,Antoine A F de Vries,Andrea Scaloni,Nico Mitro,Yu-Hua Tseng,Andrés Hidalgo,Beiyan Zhou,Jonathan R Brestoff,Katia Aquilano,Daniele Lettieri-Barbato

Journal of neuroinflammation 15:252 PubMed30180864

2018

Hippocampal Mrp8/14 signaling plays a critical role in the manifestation of depressive-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Gong,Wen-Jun Su,Zhi-Yong Cao,Yong-Jie Lian,Wei Peng,Yun-Zi Liu,Yi Zhang,Lin-Lin Liu,Ran Wu,Bo Wang,Ting Zhang,Yun-Xia Wang,Chun-Lei Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-iNOS antibody [EPR16630]

Immunoprecipitation - Anti-iNOS antibody [EPR16630] (AB205529)

ELISA - Anti-iNOS antibody [EPR16630] (AB205529)

Western blot - Anti-iNOS antibody [EPR16630] (AB205529)

Western blot - Anti-iNOS antibody [EPR16630] (AB205529)

Western blot - Anti-iNOS antibody [EPR16630] (AB205529)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise