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AB178945

Anti-iNOS antibody [EPR16635]

4

(3 Reviews)

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(275 Publications)

Anti-iNOS antibody [EPR16635] (ab178945) is a rabbit monoclonal antibody detecting iNOS in Western Blot, IP, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 270 publications

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] (AB178945)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] (AB178945)

Immunocytochemistry/Immunofluorescence analysis of RAW 264.7 non-treated and LPS treated (0.1 μg/mL) cells labelling iNOS with ab178945 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, Alexa Fluor® 594 conjugated anti-alpha tubulin (1/200). Nuclei counterstained with DAPI (blue).

Secondary antibody only controls performed on non-treated and treated cells.

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Lab

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

Blocking and diluting buffer 5% NFDM/TBST. iNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID : 11138926, PMID : 16156895, PMID : 10322315).

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 131 kDa

false

Exposure time: 180s

Indirect ELISA - Anti-iNOS antibody [EPR16635] (AB178945)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-iNOS antibody [EPR16635] (AB178945)

ELISA analysis of House mouse iNOS recombinant protein at 1000 ng/mL with ab178945. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-iNOS antibody [EPR16635] (AB178945)
  • IP

Supplier Data

Immunoprecipitation - Anti-iNOS antibody [EPR16635] (AB178945)

iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate treated with 1 μg/ml LPS for 24h with ab178945 at 1/100 dilution.

Lane 1 : RAW 264.7 whole cell lysate treated with 1 μg/ml LPS for 24h,10ug (Input).

Lane 2 : ab178945 IP in RAW 264.7 whole cell lysate treated with 1 μg/ml LPS for 24h.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab178945 in RAW 264.7 whole cell lysate treated with 1 μg/ml LPS for 24h.

Western blot was performed from the immunoprecipitate using ab178945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-iNOS antibody [EPR16635] (ab178945)

Predicted band size: 131 kDa

false

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Supplier Data

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

Lanes 1 - 2:

Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (<a href='/en-us/products/primary-antibodies/inos-antibody-epr16635-mouse-igg2b-chimeric-ab210823'>ab210823</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution

Lane 1:

Untreated L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Lane 2:

L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

Observed band size: 131 kDa

false

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Lab

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/20000 dilution

Lane 1:

Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 131 kDa

Observed band size: 131 kDa

false

Exposure time: 30s

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Unknown

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/500 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 10ug/ml lipopolysaccharides for 6 hours whole cell lysates at 15 µg

Lane 3:

THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

Lane 4:

THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml lipopolysaccharides for 3 hours whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/200000 dilution

Predicted band size: 131 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16635

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, I-ELISA, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody shows low affinity on human samples.
Based on our preliminary data, this antibody is not suitable for THP1 (Human monocytic leukemia monocyte) cell lines in WB.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>" }, "Rat": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein - Mouse": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Anti-iNOS antibody [EPR16635] (ab178945) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in I-ELISA, ICC/IF, IP and WB.

Anti-iNOS antibody [EPR16635] (ab178945) was first used in a scientific publication in 2014 and has been cited over 275 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-iNOS antibody [EPR16635] (ab178945) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-iNOS antibody [EPR16635] (ab178945) specifically detects iNOS (UniProt ID: P35228; Molecular weight: 132kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR16635 - ab213987.

Antibody clone EPR16635 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 568 (ab209027, ab209594, ab209595).

Inducible nitric oxide synthase (iNOS) plays a key role in disease by producing nitric oxide (NO), which can contribute to inflammation and tissue damage in conditions like arthritis, sepsis and cancer. EPR16635 is one of the top cited recombinant antibodies on the market with over 494 citations.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).
See full target information NOS2
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Publications (275)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 21:485 PubMed38105216

2023

Reprogramming mitochondrial metabolism of macrophages by miRNA-released microporous coatings to prevent peri-implantitis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongming Zhang,Yun Yuan,Hanxiao Xue,Runping Yu,Xiayue Jin,Xiaolin Wu,Hui Huang

Science advances 9:eadi9967 PubMed38019911

2023

Intracellular delivery of nitric oxide enhances the therapeutic efficacy of mesenchymal stem cells for myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Hao,Guangbo Ji,Meng Qian,Qiu Xuan Li,Haoyan Huang,Shiyu Deng,Pei Liu,Weiliang Deng,Yongzhen Wei,Ju He,Shusen Wang,Wenqing Gao,Tong Li,Jiansong Cheng,Jinwei Tian,Leiting Pan,Fei Gao,Zongjin Li,Qiang Zhao

Molecular medicine reports 29: PubMed37975259

2023

MCPIP1 alleviates depressive‑like behaviors in mice by inhibiting the TLR4/TRAF6/NF‑κB pathway to suppress neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Qi An,Jiejing Xia,Fanchun Pu,Shaobo Shi

BioMed research international 2023:2883623 PubMed38027040

2023

Exosomes from miR-23 Overexpressing Stromal Cells Suppress M1 Macrophage and Inhibit Calcium Oxalate Deposition in Hyperoxaluria Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Yifan,Zhang Shengli,Wang Min,Cheng Wenjie,Sun Yi,Xu Luwei,Jia Ruipeng

Bone research 11:58 PubMed37914703

2023

Combining single-cell RNA sequencing and population-based studies reveals hand osteoarthritis-associated chondrocyte subpopulations and pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Xiaofeng Jiang,Yongbing Xiao,Yuqing Zhang,Weiya Zhang,Michael Doherty,Jacquelyn Nestor,Changjun Li,Jing Ye,Tingting Sha,Houchen Lyu,Jie Wei,Chao Zeng,Guanghua Lei

Chinese medicine 18:143 PubMed37919806

2023

Xinyang tablet ameliorates sepsis-induced myocardial dysfunction by regulating Beclin-1 to mediate macrophage autophagy and M2 polarization through LncSICRNT1 targeting E3 ubiquitin ligase TRAF6.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Luo,Yuanmei Li,Liwei He,Haitao Tu,Xinfeng Lin,Fengli Zhao,Yusheng Huang,Minyong Wen,Lingjun Wang,Zhongqi Yang

Immunity, inflammation and disease 11:e1006 PubMed37904675

2023

SAMD1 attenuates antiphospholipid syndrome-induced pregnancy complications.

Applications

Unspecified application

Species

Unspecified reactive species

Ran An,Yanqi Yang,Lei Liu,Peiling Li

Nature communications 14:6963 PubMed37907455

2023

Self-promoted electroactive biomimetic mineralized scaffolds for bacteria-infected bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zixin Li,Danqing He,Bowen Guo,Zekun Wang,Huajie Yu,Yu Wang,Shanshan Jin,Min Yu,Lisha Zhu,Liyuan Chen,Chengye Ding,Xiaolan Wu,Tianhao Wu,Shiqiang Gong,Jing Mao,Yanheng Zhou,Dan Luo,Yan Liu

The Journal of international medical research 51:3000605231197071 PubMed37824732

2023

MicroRNA-22-3p alleviates atherosclerosis by mediating macrophage M2 polarization as well as inhibiting NLRP3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Bian,Haoyang Peng,Yin Wang,Hongjiang Guo,Gaofeng Shi

Frontiers in pharmacology 14:1255904 PubMed37808194

2023

Dapagliflozin promotes angiogenesis in hindlimb ischemia mice by inducing M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Yang,Wanqi Lan,Wu Liu,Tingtao Chen,Yanhua Tang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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