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AB178945

Anti-iNOS antibody [EPR16635]

4

(3 Reviews)

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(553 Publications)

Anti-iNOS antibody [EPR16635] (ab178945) is a rabbit monoclonal antibody detecting iNOS in Western Blot, IP, ICC/IF, ELISA. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 500 publications


Mouse version of this clone also available as ab210823. Same clone, same staining pattern, different species antibody backbone.
Learn more about chimeric antibodies

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] (AB178945)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] (AB178945)

Immunocytochemistry/Immunofluorescence analysis of RAW 264.7 non-treated and LPS treated (0.1 μg/mL) cells labelling iNOS with ab178945 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, Alexa Fluor® 594 conjugated anti-alpha tubulin (1/200). Nuclei counterstained with DAPI (blue).

Secondary antibody only controls performed on non-treated and treated cells.

Immunoprecipitation - Anti-iNOS antibody [EPR16635] (AB178945)
  • IP

Supplier Data

Immunoprecipitation - Anti-iNOS antibody [EPR16635] (AB178945)

iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate treated with 1 μg/ml LPS for 24h with ab178945 at 1/100 dilution.

Lane 1 : RAW 264.7 whole cell lysate treated with 1 μg/ml LPS for 24h,10ug (Input).

Lane 2 : ab178945 IP in RAW 264.7 whole cell lysate treated with 1 μg/ml LPS for 24h.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab178945 in RAW 264.7 whole cell lysate treated with 1 μg/ml LPS for 24h.

Western blot was performed from the immunoprecipitate using ab178945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-iNOS antibody [EPR16635] (ab178945)

Predicted band size: 131 kDa

false

Indirect ELISA - Anti-iNOS antibody [EPR16635] (AB178945)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-iNOS antibody [EPR16635] (AB178945)

ELISA analysis of House mouse iNOS recombinant protein at 1000 ng/mL with ab178945. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Lab

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/20000 dilution

Lane 1:

Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg

Lane 2:

RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 131 kDa

Observed band size: 131 kDa

false

Exposure time: 30s

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Supplier Data

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution

Lane 1:

Untreated L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

Lane 2:

L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 131 kDa

Observed band size: 131 kDa

false

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Lab

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

Blocking and diluting buffer 5% NFDM/TBST. iNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID : 11138926, PMID : 16156895, PMID : 10322315).

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate at 20 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 131 kDa

false

Exposure time: 180s

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)
  • WB

Unknown

Western blot - Anti-iNOS antibody [EPR16635] (AB178945)

All lanes:

Western blot - Anti-iNOS antibody [EPR16635] (ab178945) at 1/500 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 10ug/ml lipopolysaccharides for 6 hours whole cell lysates at 15 µg

Lane 3:

THP-1 (Human monocytic leukemia monocyte) whole cell lysates at 15 µg

Lane 4:

THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml lipopolysaccharides for 3 hours whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/200000 dilution

Predicted band size: 131 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16635

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB, IP, I-ELISA, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody shows low affinity on human samples.<br>Based on our preliminary data, this antibody is not suitable for THP1 (Human monocytic leukemia monocyte) cell lines in WB.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>" }, "Rat": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>To detect iNOS expression, macrophages need to be treated with LPS (0.1 - 1 ug/ml).</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein - Mouse": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Anti-iNOS antibody [EPR16635] (ab178945) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in I-ELISA, ICC/IF, IP and WB.

Anti-iNOS antibody [EPR16635] (ab178945) was first used in a scientific publication in 2014 and has been cited over 275 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-iNOS antibody [EPR16635] (ab178945) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-iNOS antibody [EPR16635] (ab178945) specifically detects iNOS (UniProt ID: P35228; Molecular weight: 132kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR16635 - ab213987.

Antibody clone EPR16635 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 568 (ab209027, ab209594, ab209595).

Inducible nitric oxide synthase (iNOS) plays a key role in disease by producing nitric oxide (NO), which can contribute to inflammation and tissue damage in conditions like arthritis, sepsis and cancer. EPR16635 is one of the top cited recombinant antibodies on the market with over 494 citations.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).
See full target information NOS2
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Publications (553)

Recent publications for all applications. Explore the full list and refine your search

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Regenerative biomaterials 12:rbaf092 PubMed41048975

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L-arginine-loaded microneedle patch enhances diabetic wound healing by regulating macrophage polarisation and mitochondrial homeostasis.

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Hong Wang,Shun Yao,Qingyun Mo,Mingyue Chen,Danfeng He,Lingfeng Yan,Chang Wang,Tao Zou,Gaoxing Luo,Jun Deng

Cell division 20:22 PubMed41039618

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FGF4 drives tumor progression in triple-negative breast cancer via IL6/STAT3-mediated macrophage M2 polarization and immune suppression.

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Xuanhe Zhang,Shushan Zhang,Yuanyuan Han

Frontiers in immunology 16:1650254 PubMed40918113

2025

The critical role of matrix metalloproteinase 9-mediated microglial polarization in perioperative neurocognitive disorders of aged rats.

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Xi Xin,Haonan Zhang,Chenyi Yang,Xinyi Wang,Lin Zhang,Ji Ma,Haiyun Wang

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

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Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

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Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

Journal of orthopaedic translation 55:62-74 PubMed40910110

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Hydrogel adhesives with a hydrodynamically induced liquid-solid transition for annular fissure sealing and inflammation modulation following microdiscectomy.

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Huan Wang,Moyan Li,Jiaojiao Yang,Zhao Liu,Shijie Shi,Dachuan Liu,Youzhi Hong,Hongjian Liu,Songfeng Chen,Jiyao Li,Song Chen,Bin Li

Bioactive materials 54:159-178 PubMed40895235

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Injectable sustained-release danshensu sodium-loaded nanoparticle hydrogel targets macrophages to improve myocardial microenvironment for myocardial infarction treatment.

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Jianqiu Yang,Jing Wang,Zhihua Zeng,Zhenyu Chen,Dingguo Wang,Yongbing Wu

Materials today. Bio 34:102198 PubMed40893373

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Neodymium-doped mesoporous silica nanoparticles promote bone regeneration via autophagy-mediated macrophage immunomodulation.

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Qing Zhang,Duraipandy Natarajan,Weijian Gao,Haokun He,Shuguang Cheng,Yin Xiao,Marco N Helder,Sujuan Zeng,Richard T Jaspers,Janak Lal Pathak

Foods (Basel, Switzerland) 14: PubMed40870813

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Longan Polysaccharide as Adjuvant for Cyclophosphamide-Induced Side Effects in Murine Model.

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Yajuan Bai,Bei Fan,Fengzhong Wang,Mingwei Zhang

Arthritis research & therapy 27:171 PubMed40859359

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KLF11 alleviates rheumatoid arthritis by regulating M1 macrophage polarization via downregulation of YAP1 expression.

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Species

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Xiaodan Liu,Fang Fang,Hongmei Duan,Chunling Wu,Haina Liu,Shuang Ding
View all publications
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Product promise

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