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Mouse Recombinant Monoclonal iNOS antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 13 publications.


Images

Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (AB210823), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (AB210823), expandable thumbnail
  • Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (AB210823), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Mouse
Tested
Tested

Tested
Tested

Species

Mouse

Dilution info

5 µg/mL

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1.00000-5.00000 µg/mL

Notes

-

Associated Products

Select an associated product type

13 products for Alternative Product

1 product for Alternative Version

Target data

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).

Alternative names

Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS, NOS2, NOS2A

Recommended products

Mouse Recombinant Monoclonal iNOS antibody. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 13 publications.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16635

Purification technique

Affinity purification Protein A

Light chain type

kappa

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-iNOS antibody [EPR16635] ab178945). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

Associated diseases and disorders

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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3 product images

  • Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823), expandable thumbnail

    Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823)

    This data was developed using Anti-iNOS antibody [EPR16635] ab178945, the same antibody clone in the original rabbit isotype.

    Lanes 1 - 2: Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Anti-iNOS antibody [EPR16635] (Anti-iNOS antibody [EPR16635] ab178945) at 1/1000 dilution

    Lane 1: Untreated L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg

    Lane 2: L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa

    Observed band size: 131 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823)

    ab210823 stained in Raw264.7 cells. Untreated and LPS treated (1ug/ml, 24 hours) cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210823 at 5μg/ml overnight at +4°C then detected with an Alexa Fluor® 488 goat anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at a 1/1000 dilution (shown in green). Alexa Fluor® 594 Anti-beta Tubulin antibody [EPR16774] ab206369 (Rabbit monoclonal [EPR16774] to beta Tubulin Alexa Fluor® 594) was used as a counterstaining at a 1/200 dilution for 1hour at room temperature (pseudo-colored red). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43μM for 1hour at room temperature.

  • Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823), expandable thumbnail

    Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab210823 (lane 1) and Anti-iNOS antibody [EPR16635] ab178945 (lane 2) overnight at 4°C. Antibody binding was detected using an anti-mouse (lane 1) and anti-rabbit (lane 2) antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-iNOS antibody [EPR16635] – Mouse IgG2b (Chimeric) (ab210823) at 5 µg

    All lanes: RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours at 10 µg

    Secondary

    Lane 1: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Lane 2: Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 131 kDa

    Observed band size: 131 kDa

    Exposure time: 8min

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