AB136918

Anti-iNOS antibody [K13-A]

Name: Anti-iNOS antibody [K13-A] (ab136918) | Abcam
Brand: Abcam Limited
SKU: AB136918
Price: 565 USD
Availability: InStock
Rating: 4 (11 reviews)

(11 Reviews)

(25 Publications)

Rabbit Monoclonal iNOS antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2.

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

3 Images

Lab

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

All lanes:

Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

All lanes:

iNOS Overexpression Lysate (Human) at 0.1 µg

Secondary

All lanes:

Goat anti Rabbit IR680 at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 140 kDa

false

Lab

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

All lanes:

Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

Lane 1:

Raw264.7 Whole Cell Lysate at 20 µg

Lane 2:

Raw264.7 Whole Cell Lysate (LPS stimulated) at 20 µg

Secondary

All lanes:

Goat anit Rabbit IR680 at 1/10000 dilution

Predicted band size: 131 kDa

Observed band size: 140 kDa,15 kDa,36 kDa,42 kDa,50 kDa

false

Unknown

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

All lanes:

Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 131 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

K13-A

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human NOS2. The exact immunogen used to generate this antibody is proprietary information.

P35228

Epitope

Antibody recognizes the epitope located between Ser1118 - Gly1129

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p>Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.

Storage buffer

pH: 8 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.32% Tris HCl

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).

See full target information NOS2

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 12:rbaf089 PubMed41048976

2025

ROS scavenging MnO nanozyme regulated immune microenvironment and affects intercellular interaction to promote wound healing in diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyuan Li,Ao Zheng,Chen Liang,Zhiyuan Mao,Tanjun Deng,Lingyan Cao,Chen Wang

Frontiers in molecular neuroscience 18:1542938 PubMed39958993

2025

Impaired peroxisomal beta-oxidation in microglia triggers oxidative stress and impacts neurons and oligodendrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Tawbeh,Catherine Gondcaille,Fatima-Ezzahra Saih,Quentin Raas,Damien Loichot,Yannick Hamon,Céline Keime,Alexandre Benani,Francesca Di Cara,Mustapha Cherkaoui-Malki,Pierre Andreoletti,Stéphane Savary

Biomolecules & therapeutics 32:546-555 PubMed39091099

2024

Aromadendrin Inhibits Lipopolysaccharide-Induced Inflammation in BEAS-2B Cells and Lungs of Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Juhyun Lee,Ji-Won Park,Jinseon Choi,Seok Han Yun,Bong Hyo Rhee,Hyeon Jeong Jeong,Hyueyun Kim,Kihoon Lee,Kyung-Seop Ahn,Hye-Gwang Jeong,Jae-Won Lee

Heliyon 9:e22932 PubMed38125474

2023

Aromadendrin inhibits PMA-induced cytokine formation/NF-κB activation in A549 cells and ovalbumin-induced bronchial inflammation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Mi Park,Ji-Won Park,Juhyun Lee,Seung-Ho Kim,Da-Yun Seo,Kyung-Seop Ahn,Sang-Bae Han,Jae-Won Lee

Antioxidants (Basel, Switzerland) 12: PubMed37891965

2023

Ellagic Acid Prevented Dextran-Sodium-Sulfate-Induced Colitis, Liver, and Brain Injury through Gut Microbiome Changes.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Ha Kim,Ji-Su Kim,Jae-Hee Kwon,In-Sook Kwun,Moon-Chang Baek,Gi-Seok Kwon,Wiramon Rungratanawanich,Byoung-Joon Song,Do-Kyun Kim,Hyo-Jung Kwon,Young-Eun Cho

International journal of molecular sciences 24: PubMed37569348

2023

Black Ginseng Extract Exerts Potentially Anti-Asthmatic Activity by Inhibiting the Protein Kinase Cθ-Mediated IL-4/STAT6 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Na Song,Jae-Won Lee,Hyung Won Ryu,Jae Kyoung Lee,Eun Sol Oh,Doo-Young Kim,Hyunju Ro,Dahye Yoon,Ji-Yoon Park,Sung-Tae Hong,Mun-Ock Kim,Su Ui Lee,Dae Young Lee

Frontiers in pharmacology 13:1075746 PubMed36532726

2022

Prenatal stress induces a depressive-like phenotype in adolescent rats: The key role of TGF-β1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Annamaria Fidilio,Margherita Grasso,Giuseppe Caruso,Nicolò Musso,Veronica Begni,Anna Privitera,Sebastiano Alfio Torrisi,Patrizia Campolongo,Stefania Schiavone,Fabio Tascedda,Gian Marco Leggio,Filippo Drago,Marco Andrea Riva,Filippo Caraci

Cancer genomics & proteomics 19:727-739 PubMed36316038

2022

Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed E M Saeed,Candela Cives-Losada,Thomas Efferth

Inflammopharmacology 30:2197-2209 PubMed35948811

2022

Nebivolol elicits a neuroprotective effect in the cuprizone model of multiple sclerosis in mice: emphasis on M1/M2 polarization and inhibition of NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Antoinette G Naeem,Reem N El-Naga,Haidy E Michel

Frontiers in pharmacology 12:809541 PubMed35002742

2021

Antioxidant Activity of Fluoxetine and Vortioxetine in a Non-Transgenic Animal Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppe Caruso,Margherita Grasso,Annamaria Fidilio,Sebastiano Alfio Torrisi,Nicolò Musso,Federica Geraci,Maria Rosaria Tropea,Anna Privitera,Fabio Tascedda,Daniela Puzzo,Salvatore Salomone,Filippo Drago,Gian Marco Leggio,Filippo Caraci

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-iNOS antibody [K13-A]

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

Western blot - Anti-iNOS antibody [K13-A] (AB136918)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

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