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Rabbit Monoclonal iNOS antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2.


Images

Western blot - Anti-iNOS antibody [K13-A] (AB136918), expandable thumbnail
  • Western blot - Anti-iNOS antibody [K13-A] (AB136918), expandable thumbnail
  • Western blot - Anti-iNOS antibody [K13-A] (AB136918), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 8
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.32% Tris HCl

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human NOS2. The exact immunogen used to generate this antibody is proprietary information. Database link P35228

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Mouse
Tested
Rat
Predicted
Transfected cell lysate - Human
Tested

Tested
Tested

Species

Transfected cell lysate - Human

Dilution info

1/2000

Notes

Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.

Species

Mouse

Dilution info

1/2000

Notes

Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109).

Alternative names

Recommended products

Rabbit Monoclonal iNOS antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human NOS2. The exact immunogen used to generate this antibody is proprietary information. Database link P35228
Clone number

K13-A

Purification technique

Affinity purification Immunogen

Epitope

Antibody recognizes the epitope located between Ser1118 - Gly1129

Concentration
Loading...
Purification notes

This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

Associated diseases and disorders

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

Product promise

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3 product images

  • Western blot - Anti-iNOS antibody [K13-A] (ab136918), expandable thumbnail

    Western blot - Anti-iNOS antibody [K13-A] (ab136918)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

    All lanes: Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

    Lane 1: Raw264.7 Whole Cell Lysate at 20 µg

    Lane 2: Raw264.7 Whole Cell Lysate (LPS stimulated) at 20 µg

    Secondary

    All lanes: Goat anit Rabbit IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 131 kDa

    Observed band size: 140 kDa, 15 kDa, 36 kDa, 42 kDa, 50 kDa

  • Western blot - Anti-iNOS antibody [K13-A] (ab136918), expandable thumbnail

    Western blot - Anti-iNOS antibody [K13-A] (ab136918)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

    All lanes: Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

    All lanes: iNOS Overexpression Lysate (Human) at 0.1 µg

    Secondary

    All lanes: Goat anti Rabbit IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 131 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-iNOS antibody [K13-A] (ab136918), expandable thumbnail

    Western blot - Anti-iNOS antibody [K13-A] (ab136918)

    All lanes: Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution

    All lanes: Mouse brain lysate at 50 µg

    Predicted band size: 131 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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