Rabbit Monoclonal iNOS antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2.
IgG
Rabbit
pH: 8
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.32% Tris HCl
Liquid
Monoclonal
WB | |
---|---|
Mouse | Tested |
Rat | Predicted |
Transfected cell lysate - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell lysate - Human | Dilution info 1/2000 | Notes Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. |
Species Mouse | Dilution info 1/2000 | Notes Incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Select an associated product type
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109).
NOS2A, NOS2, NOS2A, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS
Rabbit Monoclonal iNOS antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2.
IgG
Rabbit
pH: 8
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.32% Tris HCl
Liquid
Monoclonal
K13-A
Affinity purification Immunogen
Antibody recognizes the epitope located between Ser1118 - Gly1129
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability.
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.
INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.
INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Terms & Conditions.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Mouse IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
All lanes: Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution
Lane 1: Raw264.7 Whole Cell Lysate at 20 µg
Lane 2: Raw264.7 Whole Cell Lysate (LPS stimulated) at 20 µg
All lanes: Goat anit Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa, 15 kDa, 36 kDa, 42 kDa, 50 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab136918 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx
All lanes: Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution
All lanes: iNOS Overexpression Lysate (Human) at 0.1 µg
All lanes: Goat anti Rabbit IR680 at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 131 kDa
Observed band size: 140 kDa
All lanes: Western blot - Anti-iNOS antibody [K13-A] (ab136918) at 1/2000 dilution
All lanes: Mouse brain lysate at 50 µg
Predicted band size: 131 kDa
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