AB49999

Anti-iNOS antibody [NOS-IN]

Name: Anti-iNOS antibody [NOS-IN] (ab49999) | Abcam
Brand: Abcam Limited
SKU: AB49999
Price: 480 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(114 Publications)

Anti-iNOS antibody [NOS-IN] (ab49999) is a mouse monoclonal antibody detecting iNOS in Western Blot, ICC/IF. Suitable for Mouse.

- Over 80 publications
- Trusted since 2007

View Alternative Names

Inosl, Nos2, Inducible NO synthase, Macrophage NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Inducible NOS, iNOS, MAC-NOS

2 Images

Supplier Data

Western blot - Anti-iNOS antibody [NOS-IN] (AB49999)

This image was generated using the ascites version of the product.

Lane 1:

Western blot - Anti-iNOS antibody [NOS-IN] (ab49999) at 1/500 dilution

Lane 2:

Western blot - Anti-iNOS antibody [NOS-IN] (ab49999) at 1/1000 dilution

Lane 3:

Western blot - Anti-iNOS antibody [NOS-IN] (ab49999) at 1/2500 dilution

Lane 4:

Western blot - Anti-iNOS antibody [NOS-IN] (ab49999) at 1/5000 dilution

All lanes:

IFN-gamma/LPS-activated RAW-264.7 cell extract

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase and chemiluminescent substrate

Predicted band size: 131 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [NOS-IN] (AB49999)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [NOS-IN] (AB49999)

Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling iNOS with ab49999 at 1/2000 dilution.

Cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NOS-IN

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Mouse Nos2 aa 1100 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P29477

Specificity

This antibody reacts specifically with inducible-type nitricoxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-iNOS antibody [NOS-IN] (ab49999) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

What is the molecular weight of iNOS?
Anti-iNOS [NOS-IN] (ab49999) specifically detects a band for iNOS (UniProt: P35228) at a molecular weight of 131kDa.

Trusted by the scientific community
Anti-iNOS [NOS-IN] (ab49999) was first used in a scientific publication in 2007 and has been cited over 80 times in peer-reviewed journals.

Reviewed by scientists
Anti-iNOS [NOS-IN] (ab49999) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification notes

This antibody is purified from hybridoma cell culture.

Storage buffer

pH: 7.4 Preservative: 0.087% Sodium azide Constituents: 2.93% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7503239). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (PubMed : 16373578). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (By similarity). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (By similarity).

See full target information Nos2

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Publications (114)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 35:102299 PubMed41019489

2025

Ultrasound-activated piezoelectric scaffolds target dual Ca/NF-κB signaling pathways to orchestrate immunomodulation and osteogenesis for accelerated bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zheng,Pengfei Yan,Peng Liu,Chang Yan,Mengqi Zhao,Zuyong Wang,Xiaofei Zheng,Huajun Wang,Rongkai Zhang,Luke P Lee,Swee Hin Teoh

European journal of medical research 30:668 PubMed40708063

2025

TLR4/NF-κB-mediated M1 macrophage polarization contributes to the promotive effects of ETS2 on ulcerative colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Liu,Jianshun Yu,Jie Zhang,Wei Ye,Jiaming Yao

Frontiers in immunology 16:1576069 PubMed40589746

2025

Cordycepin, lactoferrin, and Sargassum fusiforme polysaccharides protects against RSV via M2-like macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozong Fu,Panwen Huang,Yuting Zhang,Yanchang Li,Shichang Hu

Drug design, development and therapy 19:4755-4776 PubMed40486123

2025

Alleviates Th17/Treg Immune Imbalance of Experimental Autoimmune Encephalomyelitis by Regulating the Fatty Acids Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Wu,Haoyou Xu,Hui Xia,Lilin Peng,Lulu Qin,Qian Gong,Min Zhao,Zhibing Wu,Yuanqi Zhao,Zequan Zheng

Clinical and translational medicine 15:e70315 PubMed40289345

2025

Myeloid deficiency of Z-DNA binding protein 1 restricts septic cardiomyopathy via promoting macrophage polarisation towards the M2-subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Shi,Lu He,Jie Ni,Yuyuan Zhou,Xiaohua Yu,Yao Du,Yang Li,Xi Tan,Yufang Li,Xiaoying Xu,Si Sun,Lina Kang,Biao Xu,Jibo Han,Lintao Wang

Bioactive materials 49:23-38 PubMed40110583

2025

Immune-modulated adhesive hydrogel for enhancing osteochondral graft adhesion and cartilage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Zhou,Xiongfa Ji,Yu Xue,Wenjie Yang,Guoqing Zhong,Zhiyang Zhou,Xingmei Chen,Zehua Lei,Teliang Lu,Yu Zhang,Ji Liu,Limin Ma

Life metabolism 3:loae026 PubMed39873005

2025

Phytic acid-based nanomedicine against mTOR represses lipogenesis and immune response for metabolic dysfunction-associated steatohepatitis therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Fenghua Xu,Shoujie Zhao,Yejing Zhu,Jun Zhu,Lingyang Kong,Huichen Li,Shouzheng Ma,Bo Wang,Yongquan Qu,Zhimin Tian,Junlong Zhao,Lei Liu

CNS neuroscience & therapeutics 31:e70149 PubMed39791542

2025

Protective Effects of Mdivi-1 on Cognition Disturbance Following Sepsis in Mice via Alleviating Microglia Activation and Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Hong,Li Wang,Xiaowei Zhou,Liyong Zou,Xinming Xiang,Haoyue Deng,Qinghui Li,Yue Wu,Liangming Liu,Tao Li

Journal of biochemical and molecular toxicology 38:e70062 PubMed39575578

2024

Mincle Maintains M1 Polarization of Macrophages and Contributes to Renal Aging Through the Syk/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshuang Sun,Hua Liu,Kehui Shi,Meng Wei,Hongli Jiang

Journal of nanobiotechnology 22:706 PubMed39543615

2024

Microneedle patches incorporating zinc-doped mesoporous silica nanoparticles loaded with betamethasone dipropionate for psoriasis treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Zhiguo Yuan,Shuyu Shi,Xingtao Chen,Shuangshuang Yu,Xiaoshu Qi,Tong Deng,Yifei Zhou,Dan Tang,Saihong Xu,Jue Zhang,Yingfu Jiao,Weifeng Yu,Liya Wang,Liqun Yang,Po Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-iNOS antibody [NOS-IN]

Western blot - Anti-iNOS antibody [NOS-IN] (AB49999)

Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [NOS-IN] (AB49999)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (114)

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