anti-iNOS antibody [SP126] ab239990 is a carrier free rabbit monoclonal antibody that is used in iNOS IHC and flow cytometry. Suitable for human and mouse samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Antibody clone SP126 is cited in over 30 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137253.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138603.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990)")
![Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--flow-cytometry-intracellular-img136432.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137578.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137732.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (AB239990)")
Publications
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- Chinese medicine 18:1432023Xinyang tablet ameliorates sepsis-induced myocardial dysfunction by regulating Beclin-1 to mediate macrophage autophagy and M2 polarization through LncSICRNT1 targeting E3 ubiquitin ligase TRAF6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanyuan Luo,Yuanmei Li,Liwei He,Haitao Tu,Xinfeng Lin,Fengli Zhao,Yusheng Huang,Minyong Wen,Lingjun Wang,Zhongqi YangPubMed 37919806
- Nature communications 13:13032022The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGizem Günes Günsel,Thomas M Conlon,Aicha Jeridi,Rinho Kim,Zeynep Ertüz,Niklas J Lang,Meshal Ansari,Mariia Novikova,Dongsheng Jiang,Maximilian Strunz,Mariia Gaianova,Christine Hollauer,Christina Gabriel,Ilias Angelidis,Sebastian Doll,Jeanine C Pestoni,Stephanie L Edelmann,Marlene Sophia Kohlhepp,Adrien Guillot,Kevin Bassler,Hannelore P Van Eeckhoutte,Özgecan Kayalar,Nur Konyalilar,Tamara Kanashova,Sophie Rodius,Carolina Ballester-López,Carlos M Genes Robles,Natalia Smirnova,Markus Rehberg,Charu Agarwal,Ioanna Krikki,Benoit Piavaux,Stijn E Verleden,Bart Vanaudenaerde,Melanie Königshoff,Gunnar Dittmar,Ken R Bracke,Joachim L Schultze,Henrik Watz,Oliver Eickelberg,Tobias Stoeger,Gerald Burgstaller,Frank Tacke,Vigo Heissmeyer,Yuval Rinkevich,Hasan Bayram,Herbert B Schiller,Marcus Conrad,Robert Schneider,Ali Önder YildirimPubMed 35288557
- British journal of cancer 125:176-1892021CD8 T cells inhibit metastasis and CXCL4 regulates its function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobiya Joseph,Rama Soundararajan,Suhas Vasaikar,Fei Yang,Kendra L Allton,Lin Tian,Petra den Hollander,Sevinj Isgandarova,Monika Haemmerle,Barbara Mino,Tieling Zhou,Crystal Shin,Melisa Martinez-Paniagua,Aysegul A Sahin,Jaime Rodriguez-Canales,Juri Gelovani,Jeffrey T Chang,Ghanashyam Acharya,Anil K Sood,Ignacio I Wistuba,Don L Gibbons,Luisa M Solis,Michelle C Barton,Navin Varadarajan,Jeffrey M Rosen,Xiang H Zhang,Sendurai A ManiPubMed 33795809
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | ELISA | WB | |
|---|---|---|---|---|
| Human | Tested | Expected | Not recommended | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Predicted | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Boil tissue section in Citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Incubate with primary antibody for 10 minutes at room temperature. |
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ELISA
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
4 products for Alternative Version
Target data
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7531687, PubMed:7544004). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Alternative names
Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS, NOS2, NOS2A
Recommended products
anti-iNOS antibody [SP126] ab239990 is a carrier free rabbit monoclonal antibody that is used in iNOS IHC and flow cytometry. Suitable for human and mouse samples.
- PBS only, conjugation-ready, removing anything extra from your antibodies, greater flexibility in assay design
- Antibody clone SP126 is cited in over 30 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray validation
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
SP126
- Purification technique
Affinity purification Protein A/G
- Specificity
Not suitable for mouse IHC-P.
- Concentration
- Purification notes
Purified from TCS by Protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab239990 is the carrier-free version of Anti-iNOS antibody [SP126] ab115819.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.
- Biological function summary
INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.
- Pathways
INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.
- Associated diseases and disorders
INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137253.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling iNOS with Purified Anti-iNOS antibody [SP126] ab115819 at 1/100 dilution (0.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-iNOS antibody [SP126] ab115819)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138603.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling iNOS with Purified Anti-iNOS antibody [SP126] ab115819 at 1/100 dilution (0.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer, pH 6.0. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-iNOS antibody [SP126] ab115819)![Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--flow-cytometry-intracellular-img136432.jpg "Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)")
Flow Cytometry (Intracellular) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)
Intracellular Flow Cytometry analysis of Raw264.7 cells treated with 0.1ug/ml Lipopolysaccharides for 24hours labeling iNOS with purified Anti-iNOS antibody [SP126] ab115819 at 1/200 dilution (0.48μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabelled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-iNOS antibody [SP126] ab115819).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137578.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)
Formalin-fixed, paraffin-embedded human lung stained for iNOS using Anti-iNOS antibody [SP126] ab115819 at a dilution of 1/100 in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-iNOS antibody [SP126] ab115819).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990), expandable thumbnail](https://content.abcam.com/products/images/inos-antibody-sp126-bsa-and-azide-free-ab239990--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137732.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody [SP126] - BSA and Azide free (ab239990)
Formalin-fixed, paraffin-embedded human lung stained for iNOS using Anti-iNOS antibody [SP126] ab115819 at a dilution of 1/100 in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-iNOS antibody [SP126] ab115819).
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com