AB95866

Anti-iNOS (phospho Y151) antibody

Name: Anti-iNOS (phospho Y151) antibody (ab95866) | Abcam
Brand: Abcam Limited
SKU: AB95866
Price: 535 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal iNOS phospho Y151 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NOS2 pY151.

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS (phospho Y151) antibody (AB95866)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS (phospho Y151) antibody (AB95866)

ab95866 at 1/100 dilution staining iNOS in human colon carcinoma tissue sections by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). Left image shows staining with ab95866 whilst right one shows staining with primary antibody incubated with peptide before detection.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NOS2 pY151. The exact immunogen used to generate this antibody is proprietary information.

P35228

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).

See full target information NOS2 pY151

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 12: PubMed36671041

2023

Evidence of Increased Oxidative Stress in the Placental Tissue of Women Who Suffered an Episode of Psychosis during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Oscar Fraile-Martinez,Cielo García-Montero,Sonia Rodriguez-Martín,Rosa M Funes Moñux,Coral Bravo,Juan A De Leon-Luis,Jose V Saz,Miguel A Saez,Luis G Guijarro,Guillermo Lahera,Jorge Monserrat,Fernando Mora,Javier Quintero,Julia Bujan,Natalio García-Honduvilla,Melchor Alvarez-Mon,Miguel Angel Alvarez-Mon

Antioxidants (Basel, Switzerland) 11: PubMed35453444

2022

Oxidative Stress Markers Are Associated with a Poor Prognosis in Patients with Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Oscar Fraile-Martinez,Leonel Pekarek,Cielo García-Montero,Miguel Angel Alvarez-Mon,Alejandro J Castellanos,Natalio García-Honduvilla,Julia Buján,Melchor Alvarez-Mon,Miguel A Sáez,Luis G Guijarro,Angel Asúnsolo

Antioxidants (Basel, Switzerland) 10: PubMed34207447

2021

Newborns of Mothers with Venous Disease during Pregnancy Show Increased Levels of Lipid Peroxidation and Markers of Oxidative Stress and Hypoxia in the Umbilical Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Lara Sánchez-Trujillo,Coral Bravo,Oscar Fraile-Martinez,Cielo García-Montero,Miguel A Saez,Miguel A Alvarez-Mon,Felipe Sainz,Melchor Alvarez-Mon,Julia Bujan,Juan A De Leon-Luis,Natalio García-Honduvilla

Molecular neurobiology 58:3968-3982 PubMed33904019

2021

Interleukin 17A Derived from γδ T Cell Induces Demyelination of the Brain in Angiostrongylus cantonensis Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Feng,Zongpu Zhou,Zhen Liu,Cunjing Zheng,Feng Feng,Fukang Xie,Zhong-Dao Wu

BioMed research international 2019:5320902 PubMed31886225

2019

Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Miguel Ángel Saez,Ángel Asúnsolo,Beatriz Romero,Coral Bravo,Santiago Coca,Felipe Sainz,Melchor Álvarez-Mon,Julia Buján,Natalio García-Honduvilla

Oxidative medicine and cellular longevity 2019:5164576 PubMed31281583

2019

Patients with Incompetent Valves in Chronic Venous Insufficiency Show Increased Systematic Lipid Peroxidation and Cellular Oxidative Stress Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Beatriz Romero,Ángel Asúnsolo,Mayte Sola,María J Álavrez-Rocha,Felipe Sainz,Melchor Álavrez-Mon,Julia Buján,Natalio García-Honduvilla

Nature medicine 23:551-555 PubMed28346412

2017

VISTA is an inhibitory immune checkpoint that is increased after ipilimumab therapy in patients with prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Gao,John F Ward,Curtis A Pettaway,Lewis Z Shi,Sumit K Subudhi,Luis M Vence,Hao Zhao,Jianfeng Chen,Hong Chen,Eleni Efstathiou,Patricia Troncoso,James P Allison,Christopher J Logothetis,Ignacio I Wistuba,Manuel A Sepulveda,Jingjing Sun,Jennifer Wargo,Jorge Blando,Padmanee Sharma

The Korean journal of parasitology 53:777-83 PubMed26797449

2015

Pathological Lesions and Inducible Nitric Oxide Synthase Expressions in the Liver of Mice Experimentally Infected with Clonorchis sinensis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Li Yang,Ji-Qing Shen,Yan Xue,Xiao-Bing Cheng,Zhi-Hua Jiang,Yi-Chao Yang,Ying-Dan Chen,Xiao-Nong Zhou

Alcohol (Fayetteville, N.Y.) 46:727-35 PubMed23046587

2012

Differential modulation by vascular nitric oxide synthases of the ethanol-evoked hypotension and autonomic dysfunction in female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Mahmoud M El-Mas,Ming Fan,Abdel A Abdel-Rahman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-iNOS (phospho Y151) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS (phospho Y151) antibody (AB95866)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise