Rabbit Recombinant Monoclonal iNOS antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Mouse, Mouse, Human, Recombinant full length protein - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
I-ELISA | WB | |
---|---|---|
Human | Expected | Tested |
Mouse | Expected | Tested |
Recombinant full length protein - Human | Not recommended | Tested |
Recombinant full length protein - Mouse | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes - |
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed:7504305, PubMed:7531687, PubMed:7544004, PubMed:7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed:25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed:19688109).
Nitric oxide synthase, brain
NOS2A, NOS2, NOS2A, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS
Rabbit Recombinant Monoclonal iNOS antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Mouse, Mouse, Human, Recombinant full length protein - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EP16634
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab251367 is the carrier-free version of Anti-iNOS+nNOS antibody [EP16634] ab202417.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
The iNOS and nNOS also called inducible nitric oxide synthase and neuronal nitric oxide synthase are enzymes that produce nitric oxide (NO) a signaling molecule involved in various physiological processes. iNOS and nNOS differ in their expression and regulation. iNOS is found in various tissues and is induced under inflammatory conditions while nNOS is constitutively expressed in neurons. The molecular weights of iNOS and nNOS are approximately 131 kDa and 160 kDa respectively. Both isoforms exist as homodimers facilitating the conversion of L-arginine to L-citrulline and nitric oxide.
Nitric oxide produced by these synthases acts as a neurotransmitter and an immune mediator. In neurons nNOS-derived NO modulates synaptic plasticity and cognitive function significantly affecting processes such as memory and learning. iNOS-derived NO has roles in host defense and inflammation. During immune responses iNOS expression increases and produces NO to help in pathogen destruction. Both enzymes are part of multi-protein complexes that include cofactors like NADPH FAD FMN and tetrahydrobiopterin all necessary for catalytic activity.
The nitric oxide produced by iNOS and nNOS participates in the nitric oxide signaling pathway and the cyclic GMP (cGMP) pathway. Nitric oxide activates soluble guanylate cyclase in the cGMP pathway which in turn regulates various physiological processes including vasodilation. nNOS is related to synaptophysin through neuronal pathways affecting neurotransmitter release and neuronal excitability. iNOS is associated with cytokines like TNF-α and IFN-γ in inflammatory signaling pathways having roles in cell death and immune signaling.
Nitric oxide dysregulation by iNOS and nNOS impacts numerous conditions including neurodegenerative diseases and chronic inflammation. In neurodegenerative diseases like Alzheimer's excessive NO production by nNOS can lead to neurotoxicity. Inflammatory disorders such as rheumatoid arthritis involve increased iNOS expression resulting in high levels of nitric oxide and consequent tissue damage. Both iNOS and nNOS through NO production interact with proteins such as amyloid-beta in Alzheimer’s and with various cytokines in inflammatory disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-iNOS+nNOS antibody [EP16634] ab202417, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-iNOS antibody [EP16634] (Anti-iNOS+nNOS antibody [EP16634] ab202417) at 1/10000 dilution
Lane 1: Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
Lane 2: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) treated with 0.1 mg/ml LPS for 6 hours whole cell lysate at 20 µg
All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 131 kDa
Observed band size: 131 kDa
Exposure time: 3min
This data was developed using Anti-iNOS+nNOS antibody [EP16634] ab202417, the same antibody clone in a different buffer formulation.
ELISA analysis of House mouse iNos2 recombinant protein at 1000 ng/mL with Anti-iNOS+nNOS antibody [EP16634] ab202417. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
This data was developed using Anti-iNOS+nNOS antibody [EP16634] ab202417, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
iNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID: 11138926, PMID: 16156895, PMID: 10322315).
All lanes: Western blot - Anti-iNOS+nNOS antibody [EP16634] (Anti-iNOS+nNOS antibody [EP16634] ab202417) at 1/1000 dilution
Lane 1: Mouse hippocampus tissue lysate at 20 µg
Lane 2: Mouse colon tissue lysate at 20 µg
Lane 3: Mouse colon cancer tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 131 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control.
iNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID: 11138926, PMID: 16156895, PMID: 10322315).
This data was developed using Anti-iNOS+nNOS antibody [EP16634] ab202417, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 was used as a loading control.
All lanes: Western blot - Anti-iNOS+nNOS antibody [EP16634] (Anti-iNOS+nNOS antibody [EP16634] ab202417) at 1/1000 dilution
Lane 1: C-Myc/DDK tagged full length human NOS1 (nNOS) recombinant protein at 10 ng
Lane 2: C-Myc/DDK tagged full length human NOS2 (iNOS) recombinant protein at 10 ng
Lane 3: C-Myc/DDK tagged full length human NOS3 (eNOS) recombinant protein at 10 ng
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 130 kDa, 160 kDa
Exposure time: 20s
Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 was used as a loading control.
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