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Rabbit Recombinant Monoclonal INPP4A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (AB247910), expandable thumbnail
  • Western blot - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (AB247910), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (AB247910), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWB
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Expected
Rat
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) (PubMed:15716355, PubMed:20463662). Catalyzes also inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate (By similarity). Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (By similarity) (PubMed:30071275). May protect neurons from excitotoxic cell death by regulating the synaptic localization of cell surface N-methyl-D-aspartate-type glutamate receptors (NMDARs) and NMDAR-mediated excitatory postsynaptic current (By similarity). Isoform 4. Displays no 4-phosphatase activity for PtdIns(3,4)P2, Ins(3,4)P2, or Ins(1,3,4)P3.

Alternative names

Inositol polyphosphate-4-phosphatase type I A, Inositol polyphosphate 4-phosphatase type I, INPP4A

Recommended products

Rabbit Recombinant Monoclonal INPP4A antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP3425(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247910 is the carrier-free version of Anti-INPP4A antibody [EP3425(2)] ab109622.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

INPP4A also known as inositol polyphosphate 4-phosphatase type I is an enzyme that specifically dephosphorylates the D-4 position phosphate of phosphoinositides. It has a molecular mass of about 105 kDa. This enzyme is expressed in several tissues including brain and platelets indicating its involvement in various cellular processes. INPP4A is significant in controlling the intracellular levels of phosphoinositide messengers and plays an important role in signaling pathways.

Biological function summary

INPP4A functions to regulate phosphoinositide signaling which is critical in controlling cell growth apoptosis and other cellular processes. It acts by reducing levels of phosphatidylinositol 34-bisphosphate an important lipid signaling molecule. INPP4A does not appear to form part of a larger complex but operates within a network of other phosphatases and kinases that together maintain lipid signaling homeostasis.

Pathways

INPP4A interacts mainly in the phosphoinositide 3-kinase (PI3K) signaling pathway. This pathway is important for various cellular functions such as metabolism cell growth and survival. INPP4A modifies the activity of related proteins within this pathway like PI3K by reducing phosphatidylinositol 34-bisphosphate levels therefore controlling downstream signaling. It also connects with other phosphoinositide phosphatases like PTEN to finely tune the signaling balance.

Associated diseases and disorders

INPP4A is linked to neurological disorders and cancer. In cancer the dysfunction of INPP4A can lead to abnormal cell growth due to dysregulated PI3K signaling. This enzyme's activity is central in maintaining normal phosphoinositide turnover and its interactions with proteins such as PTEN underline its importance in preventing uncontrolled cell proliferation. For neurological disorders alterations in INPP4A function may affect synapse function and neuronal survival contributing to neurodegenerative processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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