Rabbit Recombinant Monoclonal INPP4B antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Not recommended | Tested | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes Perform heat mediated antigen retrieval by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified version to be used at 1/100 dilution. |
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PubMed:24070612, PubMed:24591580). Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PubMed:24591580). The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival (PubMed:19647222, PubMed:24070612).
Inositol polyphosphate 4-phosphatase type II, INPP4B
Rabbit Recombinant Monoclonal INPP4B antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 12 publications.
Inositol polyphosphate 4-phosphatase type II, INPP4B
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR3108Y
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
INPP4B also known as inositol polyphosphate-4-phosphatase type II has a molecular mass of approximately 105 kDa. This enzyme primarily localizes in the cytoplasm but also presents in the nucleus. It catalyzes the hydrolysis of the 4-phosphate group from phosphoinositides converting phosphatidylinositol (34)-bisphosphate (PI(34)P2) to phosphatidylinositol 3-phosphate (PI3P). INPP4B expression is noticeable in various tissues including the prostate thyroid and breast suggesting its diverse roles in different physiological processes.
INPP4B participates in regulating intracellular signaling pathways and maintaining cellular homeostasis. It is a negative regulator of the PI3K/AKT signaling cascade a critical pathway controlling cell growth proliferation survival and metabolism. INPP4B acts as a tumor suppressor modulating signals that impact cellular transformation and tumorigenesis. While not known to form a complex itself it indirectly influences complexes within the cascade through its regulatory functions.
INPP4B contributes to the PI3K/AKT and mTOR pathways. It regulates PI3K/AKT signaling by decreasing levels of PI(34)P2 therefore modulating AKT activity. This action makes it an essential component in controlling mTOR signaling which affects protein synthesis and autophagy. INPP4B closely interacts with other signaling proteins like PTEN also a phosphatase which further lowers PI3K signaling by dephosphorylating PI(345)P3 to PI(45)P2.
INPP4B has notable links to cancer and insulin resistance. In some cancers like breast and prostate reduced INPP4B expression correlates with increased aggressive phenotypic markers and poor outcomes indicating its tumor-suppressive role. Its regulation of the PI3K/AKT pathway connects it to insulin signaling where dysregulated pathways can lead to insulin resistance a critical factor in type 2 diabetes. INPP4B interacts with proteins involved in these diseases like AKT and PTEN which further influence and exacerbate the pathology in these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
INPP4B is lowly expressed in PANC-1 (PMID: 29952716)
All lanes: Western blot - Anti-INPP4B antibody [EPR3108Y] (ab81269) at 1/1000 dilution
Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Human skeletal muscle lysate at 20 µg
Lane 3: A-204 (Human muscle rhabdomyosarcoma) whole cell lysate at 20 µg
Lane 4: PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 105 kDa
Observed band size: 107 kDa
Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling INPP4B with Purified ab81269 at 1:50 dilution (2.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling INPP4B with Purified ab81269 at 1:50 dilution (2.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling INPP4B with Purified ab81269 at 1:50 dilution (2.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling INPP4B with purified ab81269 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
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