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AB191520

Anti-INPP5E antibody

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(3 Publications)

Rabbit Polyclonal INPP5E antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Phosphatidylinositol polyphosphate 5-phosphatase type IV.

View Alternative Names

Phosphatidylinositol polyphosphate 5-phosphatase type IV, 72 kDa inositol polyphosphate 5-phosphatase, Inositol polyphosphate-5-phosphatase E, INPP5E

1 Images
Western blot - Anti-INPP5E antibody (AB191520)
  • WB

Supplier Data

Western blot - Anti-INPP5E antibody (AB191520)

All lanes:

Western blot - Anti-INPP5E antibody (ab191520)

Lane 1:

HEK293T whole cell lysate

Lane 2:

sp2/0 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Phosphatidylinositol polyphosphate 5-phosphatase type IV. The exact immunogen used to generate this antibody is proprietary information.

Q9NRR6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/5", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab191520 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INPP5E also known as type I inositol polyphosphate-5-phosphatase plays a significant role as an enzyme regulating phosphoinositide signaling. This protein has a molecular mass of approximately 72 kDa. INPP5E cleaves the 5-phosphate from phosphoinositides affecting cellular processes. It is widely expressed in various tissues with notable presence in the brain and testis indicating its importance in these tissue types.
Biological function summary

INPP5E is involved in maintaining phosphoinositide balance within cells which regulates cellular signaling pathways. It is essential for the function of primary cilia by controlling ciliary membrane composition. This protein often interacts with components of the ciliary transition zone forming part of the complex that regulates trafficking proteins and movement.

Pathways

INPP5E engages in the phosphoinositide signaling pathway and the ciliary signaling pathway. It influences the activity of proteins such as phosphatidylinositol and adenylyl cyclase playing a role in signaling cascades that affect cell metabolism and spatial orientation. Disruption in its function can alter these signaling pathways demonstrating its importance in cellular communication and function.

Studies link INPP5E to Joubert syndrome and Meckel syndrome conditions affecting ciliary function. These ciliopathies are marked by abnormal brain development and a range of other symptoms. INPP5E interacts with proteins like ARL13B and CEP290 which are also associated with these disorders highlighting its integral role in maintaining healthy ciliary architecture and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (By similarity) (PubMed : 10764818). Specific for lipid substrates, inactive towards water soluble inositol phosphates (PubMed : 10764818). Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability (By similarity).
See full target information Phosphatidylinositol polyphosphate 5-phosphatase type IV
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 236:4050-4065 PubMed33174204

2020

GBE attenuates arsenite-induced hepatotoxicity by regulating E2F1-autophagy-E2F7a pathway and restoring lysosomal activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Chen,Dapeng Wang,Baofei Sun,Chunyan Liu,Kai Zhu,Aihua Zhang

Journal of lipid research 61:1004-1013 PubMed32371566

2020

Mass spectrometry imaging and LC/MS reveal decreased cerebellar phosphoinositides in Niemann-Pick type C1-null mice.

Applications

Unspecified application

Species

Unspecified reactive species

Koralege C Pathmasiri,Melissa R Pergande,Fernando Tobias,Rima Rebiai,Avia Rosenhouse-Dantsker,Ernesto R Bongarzone,Stephanie M Cologna

Molecular medicine reports 17:2991-2997 PubMed29257251

2017

Upregulation of miR‑598 promotes cell proliferation and cell cycle progression in human colorectal carcinoma by suppressing INPP5E expression.

Applications

Unspecified application

Species

Unspecified reactive species

Kun-Ping Li,Yong-Ping Fang,Jin-Qi Liao,Jin-Dong Duan,Li-Guang Feng,Xiao-Zai Luo,Zhi-Jian Liang
View all publications
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