Anti-INPP5E antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP5E antibody (AB236108)

Paraffin-embedded human pancreatic tissue stained for INPP5E using ab236108 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-INPP5E antibody (AB236108)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for INPP5E (green) using ab236108 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488^® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP5E antibody (AB236108)

Paraffin-embedded human heart tissue stained for INPP5E using ab236108 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.