Rabbit Polyclonal INPP5F antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human INPP5F aa 550-950.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human INPP5F aa 550-950. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2H2
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Inositol 4-phosphatase which mainly acts on phosphatidylinositol 4-phosphate. May be functionally linked to OCRL, which converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol, for a sequential dephosphorylation of phosphatidylinositol 4,5-bisphosphate at the 5 and 4 position of inositol, thus playing an important role in the endocytic recycling (PubMed:25869669). Regulator of TF:TFRC and integrins recycling pathway, is also involved in cell migration mechanisms (PubMed:25869669). Modulates AKT/GSK3B pathway by decreasing AKT and GSK3B phosphorylation (PubMed:17322895). Negatively regulates STAT3 signaling pathway through inhibition of STAT3 phosphorylation and translocation to the nucleus (PubMed:25476455). Functionally important modulator of cardiac myocyte size and of the cardiac response to stress (By similarity). May play a role as negative regulator of axon regeneration after central nervous system injuries (By similarity).
Alternative names
KIAA0966, SAC2, MSTP007, MSTP047, INPP5F, Phosphatidylinositide phosphatase SAC2, Inositol polyphosphate 5-phosphatase F, Sac domain-containing inositol phosphatase 2, Sac domain-containing phosphoinositide 4-phosphatase 2, hSAC2
Recommended products
Rabbit Polyclonal INPP5F antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human INPP5F aa 550-950.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human INPP5F aa 550-950. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y2H2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
INPP5F also known as phosphatidylinositol-45-bisphosphate 5-phosphatase F functions as a lipid phosphatase that removes 5-phosphate groups from phosphoinositides. These molecules are important for cellular signaling pathways. INPP5F has a molecular mass of approximately 75 kDa. It is expressed in various tissues with higher levels found in the brain and heart. This suggests its importance in specific cellular tissues for specialized roles.
- Biological function summary
Removing phosphate groups from phosphoinositides impacts cell signaling and membrane dynamics. INPP5F modulates cellular processes including cytoskeletal organization and vesicle trafficking through its phosphatase activity. It does not appear to be part of any stable complexes but regulates its substrates and influences processes through its enzymatic action.
- Pathways
INPP5F participates in important signaling pathways such as the phosphatidylinositol signaling system and the PI3K-Akt pathway. These pathways are essential for controlling cell growth and survival. INPP5F is functionally related to other phosphatases including PTEN which also act within these pathways to balance signaling activities for normal cellular function.
- Associated diseases and disorders
INPP5F has been linked to cardiovascular diseases and certain neurodegenerative disorders. Disturbances in its activity can result in altered signaling impacting heart function and brain health. It connects to proteins like Akt through the PI3K pathway in cardiovascular contexts whereas disruptions may influence Alzheimer's disease pathology by affecting cellular signaling and regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-INPP5F antibody (ab95990)
7.5% SDS PAGE
All lanes: Western blot - Anti-INPP5F antibody (ab95990) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 128 kDa
Western blot - Anti-INPP5F antibody (ab95990)
INPP5F Western blot staining using rabbit Anti-INPP5F antibody
Western blot: Rabbit Polyclonal to INPP5F ab95990 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20,000 dilution, shown in magenta.
A band was observed at 170 kDa in Wild-type A549 cell lysates with no signal observed at this size in INPP5F knockout A549 cell line.
To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100pc LICOR in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. /p>
Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution./p>
All lanes: Western blot - Anti-INPP5F antibody (ab95990) at 1/1000 dilution
Lane 1: Wild-type A549 at 20 µg
Lane 2: INPP5F knockout A549 at 20 µg
Lane 3: Human Brain at 20 µg
Lane 4: U-87 MG at 20 µg
SecondaryAll lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 170 kDa
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