Anti-INPP5F antibody [MJF-D29759-51] - BSA and Azide free

• WB

Lab

Western blot - Anti-INPP5F antibody [MJF-D29759-51] - BSA and Azide free (AB326359)

This data was developed using ab325711, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HeLa.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the lower MW band at approximately 60 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HeLa.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the lower MW band at approximately 60 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-INPP5F antibody [MJF-D29759-51] (<a href='/en-us/products/primary-antibodies/inpp5f-antibody-mjf-d29759-51-ab325711'>ab325711</a>) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-INPP5F antibody [MJF-D29759-51] - BSA and Azide free (AB326359)

This data was developed using ab325711, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : THP-1.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the lower MW band at approximately 60 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-INPP5F antibody [MJF-D29759-51] (<a href='/en-us/products/primary-antibodies/inpp5f-antibody-mjf-d29759-51-ab325711'>ab325711</a>) at 1/1000 dilution

Lane 1:

SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-INPP5F antibody [MJF-D29759-51] - BSA and Azide free (AB326359)

This data was developed using ab325711, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the lower MW band at approximately 60 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-INPP5F antibody [MJF-D29759-51] (<a href='/en-us/products/primary-antibodies/inpp5f-antibody-mjf-d29759-51-ab325711'>ab325711</a>) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

A375 transfected with siRNA specifically targeting INPP5F whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

false

Exposure time: 169s

• WB

Lab

Western blot - Anti-INPP5F antibody [MJF-D29759-51] - BSA and Azide free (AB326359)

This data was developed using ab325711, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, testis.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the lower MW band at approximately 60 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-INPP5F antibody [MJF-D29759-51] (<a href='/en-us/products/primary-antibodies/inpp5f-antibody-mjf-d29759-51-ab325711'>ab325711</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat cerebral cortex tissue lysate at 20 µg

Lane 4:

Rat cerebellum tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Lane 6:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

true

Exposure time: 180s