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AB250429

Anti-INSC antibody [EPR13470] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal INSC antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Protein inscuteable homolog, INSC

2 Images
Western blot - Anti-INSC antibody [EPR13470] - BSA and Azide free (AB250429)
  • WB

Supplier Data

Western blot - Anti-INSC antibody [EPR13470] - BSA and Azide free (AB250429)

This data was developed using ab181210, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-INSC antibody [EPR13470] (<a href='/en-us/products/primary-antibodies/insc-antibody-epr13470-ab181210'>ab181210</a>) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 63 kDa

false

Western blot - Anti-INSC antibody [EPR13470] - BSA and Azide free (AB250429)
  • WB

Supplier Data

Western blot - Anti-INSC antibody [EPR13470] - BSA and Azide free (AB250429)

This data was developed using ab181210, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-INSC antibody [EPR13470] (<a href='/en-us/products/primary-antibodies/insc-antibody-epr13470-ab181210'>ab181210</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

293 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13470

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250429 is the carrier-free version of ab181210.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

INSC also known as Inscuteable homolog is a protein encoded by the INSC gene. This protein has a molecular weight of approximately 58 kDa. INSC plays a mechanical role in the asymmetric division of cells. It is mainly expressed in the neural tissues where it influences neural differentiation by orienting the mitotic spindle. INSC acts as a scaffold protein and integrates various cellular components important for orienting asymmetric cell division.
Biological function summary

INSC interacts with partner proteins to influence important processes in the neural system. It forms part of a complex that includes Par3 and aPKC which are essential for cellular polarity mechanisms. INSC's role in neural progenitor cells impacts how these cells divide and differentiate affecting brain development. This protein influences cellular orientation and division aiding in the maintenance of a balance between stem cell renewal and differentiation.

Pathways

The function of INSC integrates into the Wnt and Notch signaling pathways. It regulates these pathways' impact on neural development by influencing cell polarity and division. INSC interacts with Wnt pathway-related proteins like Disheveled (Dvl) and components of Notch signaling to ensure proper cell fate determination. These interactions regulate how progenitor cells in the neural tissues choose between proliferating or differentiating into specialized cells.

INSC may relate to neurodevelopmental disorders and certain types of cancer. Abnormalities in INSC expression or function can disrupt neural development potentially leading to developmental disorders like microcephaly. In addition altered INSC activity influences pathways associated with glioblastomas where its interaction with proteins like Par3 and aPKC becomes dysregulated. Understanding these associations is important for developing insights into disease mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as an adapter linking the Par3 complex to the GPSM1/GPSM2 complex (PubMed : 16458856). Involved in spindle orientation during mitosis. May regulate cell proliferation and differentiation in the developing nervous system. May play a role in the asymmetric division of fibroblasts and participate in the process of stratification of the squamous epithelium (By similarity).
See full target information INSC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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