Anti-INSL3 antibody [EPR14834(2)]

• IP

Supplier Data

Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] (AB199536)

INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human testis whole cell lysate 10 μg (Input). Lane 2 : ab199536 IP in Human testis whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] (ab199536)

Predicted band size: 14 kDa

false

• WB

Supplier Data

Western blot - Anti-INSL3 antibody [EPR14834(2)] (AB199536)

INSL3 is expressed in prenatal and postnatal Leydig cells. Human fetal heart, kidney, spleen, NCCIT, A431 & Jurkat cells represent negative controls in this experiment.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

Human fetal spleen lysate at 10 µg

Lane 6:

NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate at 10 µg

Lane 7:

A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Lane 8:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

Exposure time: 3min