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AB251284

Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal INSL3 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse samples.

View Alternative Names

RLF, RLNL, INSL3, Insulin-like 3, Leydig insulin-like peptide, Relaxin-like factor, Ley-I-L

2 Images
Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (AB251284)
  • IP

Supplier Data

Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (AB251284)

This data was developed using ab199536, the same antibody clone in a different buffer formulation.

INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human testis whole cell lysate 10 μg (Input). Lane 2 : ab199536 IP in Human testis whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] (<a href='/en-us/products/primary-antibodies/insl3-antibody-epr148342-ab199536'>ab199536</a>)

Predicted band size: 14 kDa

false

Western blot - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (AB251284)
  • WB

Supplier Data

Western blot - Anti-INSL3 antibody [EPR14834(2)] - BSA and Azide free (AB251284)

This data was developed using ab199536, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

INSL3 is expressed in prenatal and postnatal Leydig cells. Human fetal heart, kidney, spleen, NCCIT, A431, and Jurkat cells represent negative controls in this experiment.

All lanes:

Western blot - Anti-INSL3 antibody [EPR14834(2)] (<a href='/en-us/products/primary-antibodies/insl3-antibody-epr148342-ab199536'>ab199536</a>) at 1/5000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Human testis lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

Human fetal spleen lysate at 10 µg

Lane 6:

NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate at 10 µg

Lane 7:

A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Lane 8:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14834(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251284 is the carrier-free version of ab199536.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The INSL3 gene encodes insulin-like peptide 3 also known as Relaxin-like peptide. It is a hormone belonging to the insulin superfamily with a molecular mass of approximately 12 kDa. INSL3 is expressed primarily in the Leydig cells of the testes in males but can also be found in the theca cells of the ovaries in females. This peptide hormone plays a significant role in male and female reproductive physiology by acting through its receptor RXFP2 (Relaxin family peptide receptor 2).
Biological function summary

Insulin-like peptide 3 influences the development of male gonads and the descent of testes during embryonic development making it important for reproductive success. INSL3 also functions in ovarian follicle maturation in females. This hormone operates by binding to the RXFP2 receptor forming a complex that triggers signaling cascades leading to tissue development and differentiation. However the extent of its involvement in female reproductive processes remains under investigation.

Pathways

Insulin-like peptide 3 functions mainly through the cAMP signaling pathway via its interaction with RXFP2 which plays an important role in regulating cell proliferation and differentiation. INSL3 influences the reproductive hormone axis working alongside other hormones like testosterone and luteinizing hormone to coordinate testicular descent and manage reproductive function. Its signaling pathway impacts various stages of gonadal development and maturation.

Mutations or dysfunctions in the INSL3 or RXFP2 gene may result in cryptorchidism a condition where one or both testicles fail to descend properly affecting fertility. This condition involves the hormone-testosterone pathway highlighting the critical interactions between INSL3 and testosterone synthesis. Additionally altered levels of INSL3 could be linked to conditions related to ovarian function disorders reflecting its importance in both male and female reproductive health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.
See full target information INSL3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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