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Rabbit Recombinant Monoclonal INSM1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

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Images

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104), expandable thumbnail
  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104), expandable thumbnail
  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104), expandable thumbnail
  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104), expandable thumbnail
  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Tested
Expected
Mouse
Not recommended
Not recommended
Not recommended
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-[TG][TC][TC][TT][GA]GGG[CG]A-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (PubMed:23721412). Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation.

Alternative names

IA1, INSM1, Insulinoma-associated protein 1, Zinc finger protein IA-1

Recommended products

Rabbit Recombinant Monoclonal INSM1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23199-37-6-1
Purification technique
Affinity purification Protein A
Specificity

For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization.

Human and rat are recommended based on IHC results. we do not guarantee western blot for human and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The INSM1 protein also known as Insulinoma-Associated 1 is a zinc finger transcription factor with a molecular weight of about 60 kDa. This protein is expressed in developing and mature neuroendocrine tissues. It plays an important role in the development of the nervous system and serves as a marker for neuroendocrine differentiation. Researchers can use INSM1 immunohistochemistry (IHC) to identify neuroendocrine tumors.

Biological function summary

The transcription factor INSM1 regulates key genetic programs necessary for neuronal differentiation. It interacts with other transcription factors and is involved in the repression of specific neuronal genes. During neural development it promotes the maturation of progenitor cells into specialized neuroendocrine cells. INSM1 acts as part of a transcriptional complex that modulates important developmental genes in the central nervous system.

Pathways

INSM1 plays a critical role in the Notch signaling and Hedgehog pathways. In the Notch signaling pathway it contributes to neuronal differentiation by regulating the expression of Hes1 and other Notch target genes. The Hedgehog pathway involvement suggests a role in mediating signals necessary for cellular patterning during neurogenesis. INSM1 interacts with proteins like Hes1 and Gli1 driving the cascade of events that lead to neuronal development and differentiation.

Associated diseases and disorders

INSM1 relates to neuroendocrine tumors and diabetes. In neuroendocrine tumors INSM1 expression assists in tumor identification and is a diagnostic marker due to its specificity for these tissues. Additionally disruptions in INSM1 function can impact pancreatic beta-cell development highlighting its relevance to diabetes. Its relation to proteins such as PDX-1 and Nkx6.1 is important in understanding its involvement in disease mechanisms particularly in neuroendocrine and metabolic disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104), expandable thumbnail

    Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST Negative control: heart (human protein atlas database)
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104) at 1/1000 dilution

    Lane 1: Mouse pituitary tissue lysate 50 μg

    Lane 2: Mouse heart tissue lysate 50 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 70 kDa

    Exposure time: 180s

  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104), expandable thumbnail

    Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islets of human pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104), expandable thumbnail

    Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104)

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islet of rat pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104), expandable thumbnail

    Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104)

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islet of mouse pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

  • Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104), expandable thumbnail

    Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling Insm1 with ab305104 at 1/2000 (0.256 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on HEK-293T cells transfected with a mouse Insm1 expression vector containing a myc-His tag cell pellet, and no staining on HEK-293T cells transfected with empty vector containing a his tag cell pellet. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

  • Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104), expandable thumbnail

    Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST The observed band is consistent to what has been described in literature(PMID:12079283)
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104) at 1/1000 dilution

    Lane 1: Beta-TC-6 (mouse pancreas insinoma beta cell) cytoplasmic fraction 40 µg

    Lane 2: Beta-TC-6 nuclear fraction 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 70 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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