AB305104

Anti-INSM1 antibody [EPR23199-37-6-1]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal INSM1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

IA1, INSM1, Insulinoma-associated protein 1, Zinc finger protein IA-1

6 Images

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islets of human pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling Insm1 with ab305104 at 1/2000 (0.256 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on HEK-293T cells transfected with a mouse Insm1 expression vector containing a myc-His tag cell pellet, and no staining on HEK-293T cells transfected with empty vector containing a his tag cell pellet. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islet of mouse pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islet of rat pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

Supplier Data

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : heart (human protein atlas database) Exposure time : 180 seconds

All lanes:

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104) at 1/1000 dilution

Lane 1:

Mouse pituitary tissue lysate 50 μg

Lane 2:

Mouse heart tissue lysate 50 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The observed band is consistent to what has been described in literature(PMID : 12079283) Exposure time : 180 seconds

All lanes:

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (ab305104) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insinoma beta cell) cytoplasmic fraction 40 µg

Lane 2:

Beta-TC-6 nuclear fraction 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

Carrier free

Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23199-37-6-1

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization.

Human and rat are recommended based on IHC results. we do not guarantee western blot for human and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "For IHC-P, this antibody is recommended to incubate at 4°C overnight, a shorter incubation time may cause unexpected subcellular localization. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The INSM1 protein also known as Insulinoma-Associated 1 is a zinc finger transcription factor with a molecular weight of about 60 kDa. This protein is expressed in developing and mature neuroendocrine tissues. It plays an important role in the development of the nervous system and serves as a marker for neuroendocrine differentiation. Researchers can use INSM1 immunohistochemistry (IHC) to identify neuroendocrine tumors.

Biological function summary

The transcription factor INSM1 regulates key genetic programs necessary for neuronal differentiation. It interacts with other transcription factors and is involved in the repression of specific neuronal genes. During neural development it promotes the maturation of progenitor cells into specialized neuroendocrine cells. INSM1 acts as part of a transcriptional complex that modulates important developmental genes in the central nervous system.

Pathways

INSM1 plays a critical role in the Notch signaling and Hedgehog pathways. In the Notch signaling pathway it contributes to neuronal differentiation by regulating the expression of Hes1 and other Notch target genes. The Hedgehog pathway involvement suggests a role in mediating signals necessary for cellular patterning during neurogenesis. INSM1 interacts with proteins like Hes1 and Gli1 driving the cascade of events that lead to neuronal development and differentiation.

INSM1 relates to neuroendocrine tumors and diabetes. In neuroendocrine tumors INSM1 expression assists in tumor identification and is a diagnostic marker due to its specificity for these tissues. Additionally disruptions in INSM1 function can impact pancreatic beta-cell development highlighting its relevance to diabetes. Its relation to proteins such as PDX-1 and Nkx6.1 is important in understanding its involvement in disease mechanisms particularly in neuroendocrine and metabolic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-[TG][TC][TC][TT][GA]GGG[CG]A-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4' (PubMed : 23721412). Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation.

See full target information INSM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:428 PubMed37664660

2023

Multiple synchronous primary malignant neoplasms: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Hao,Lihui Zhang,Chuanjie Xu,Min Jiang,Geyi Zhu,Jie Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-INSM1 antibody [EPR23199-37-6-1]

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (AB305104)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise