Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free

• IHC

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free (AB305073)

This data was developed using ab305104, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islets of human pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free (AB305073)

This data was developed using ab305104, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling Insm1 with ab305104 at 1/2000 (0.256 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on HEK-293T cells transfected with a mouse Insm1 expression vector containing a myc-His tag cell pellet, and no staining on HEK-293T cells transfected with empty vector containing a his tag cell pellet. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free (AB305073)

This data was developed using ab305104, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islet of mouse pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free (AB305073)

This data was developed using ab305104, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Insm1 with ab305104 at 1/100 (5.12 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on islet of rat pancreas. The section was incubated with ab305104 at 4ЎгC overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 10 mins

• WB

Supplier Data

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free (AB305073)

This data was developed using ab305104, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : heart (human protein atlas database) 180 seconds Exposure time :

All lanes:

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (<a href='/en-us/products/primary-antibodies/insm1-antibody-epr23199-37-6-1-ab305104'>ab305104</a>) at 1/1000 dilution

Lane 1:

Mouse pituitary tissue lysate 50 μg

Lane 2:

Mouse heart tissue lysate 50 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] - BSA and Azide free (AB305073)

This data was developed using ab305104, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : The observed band is consistent to what has been described in literature(PMID : 12079283) 180 seconds Exposure time :

All lanes:

Western blot - Anti-INSM1 antibody [EPR23199-37-6-1] (<a href='/en-us/products/primary-antibodies/insm1-antibody-epr23199-37-6-1-ab305104'>ab305104</a>) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insinoma beta cell) cytoplasmic fraction 40 µg

Lane 2:

Beta-TC-6 nuclear fraction 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s