Anti-INSM1 antibody [EPR28316-7] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-INSM1 antibody [EPR28316-7] - BSA and Azide free (AB314447)

This data was developed using ab314446, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Beta-TC-6 (mouse pancreas insulinoma beta cell) cells labelling INSM1 with ab314446 at 1/50 (10.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in Beta-TC-6 cell line.Negative control : Mouse splenocytes. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• WB

Supplier Data

Western blot - Anti-INSM1 antibody [EPR28316-7] - BSA and Azide free (AB314447)

This data was developed using ab314446, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : mouse spleen. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-INSM1 antibody [EPR28316-7] (<a href='/en-us/products/primary-antibodies/insm1-antibody-epr28316-7-ab314446'>ab314446</a>) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insinoma beta cell) whole cell lysate at 70 µg

Lane 2:

Mouse spleen tissue lysate at 70 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-INSM1 antibody [EPR28316-7] - BSA and Azide free (AB314447)

This data was developed using ab314446, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-INSM1 antibody [EPR28316-7] (<a href='/en-us/products/primary-antibodies/insm1-antibody-epr28316-7-ab314446'>ab314446</a>) at 1/1000 dilution

Lane 1:

Beta-TC-6 (mouse pancreas insinoma beta cell) nuclear fraction at 70 µg

Lane 2:

Beta-TC-6 (mouse pancreas insinoma beta cell) cytoplasmic fraction at 70 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 70 kDa

false

Exposure time: 136s

• Dot

Supplier Data

Dot Blot - Anti-INSM1 antibody [EPR28316-7] - BSA and Azide free (AB314447)

This data was developed using ab314446, the same antibody clone in a different buffer formulation. Dot blot analysis of INSM1 using ab314446 at 1 : 1000 (0.517 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 20,000 dilution. Exposure time : 70 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST