Rabbit Polyclonal Insulin antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 45 publications.
Images
Publications
Filter by
- Cell reports 40:1114232022Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I iPSCs for β cell replacement.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaniero Chimienti,Tania Baccega,Silvia Torchio,Fabio Manenti,Silvia Pellegrini,Alessandro Cospito,Angelo Amabile,Marta Tiffany Lombardo,Paolo Monti,Valeria Sordi,Angelo Lombardo,Mauro Malnati,Lorenzo PiemontiPubMed 36170817
- JCI insight 7:202214-3-3ζ Constrains insulin secretion by regulating mitochondrial function in pancreatic β cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYves Mugabo,Cheng Zhao,Ju Jing Tan,Anindya Ghosh,Scott A Campbell,Evgenia Fadzeyeva,Frédéric Paré,Siew Siew Pan,Maria Galipeau,Julia Ast,Johannes Broichhagen,David J Hodson,Erin E Mulvihill,Sophie Petropoulos,Gareth E LimPubMed 35298439
- Cell transplantation 31:96368972210814832022The Roles of Different Multigene Combinations of , , , , and in the Reprogramming of Canine ADSCs Into IPCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDengke Gao,Pengxiu Dai,Zhixin Fan,Jinglu Wang,Yihua ZhangPubMed 35236160
- Cell reports 38:1104252022An HNF1α truncation associated with maturity-onset diabetes of the young impairs pancreatic progenitor differentiation by antagonizing HNF1β function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna-Maria Cujba,Mario E Alvarez-Fallas,Sergio Pedraza-Arevalo,Anna Laddach,Maggie H Shepherd,Andrew T Hattersley,Fiona M Watt,Rocio SanchoPubMed 35235779
- Small (Weinheim an der Bergstrasse, Germany) 18:e21048992021A Safe, Fibrosis-Mitigating, and Scalable Encapsulation Device Supports Long-Term Function of Insulin-Producing Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanjun Liu,James A Flanders,Long-Hai Wang,Qingsheng Liu,Daniel T Bowers,Kai Wang,Alan Chiu,Xi Wang,Alexander U Ernst,Kaavian Shariati,Julia S Caserto,Benjamin Parker,Daqian Gao,Mitchell D Plesser,Lars G Grunnet,Claude Rescan,Rodrigo Pimentel Carletto,Louise Winkel,Juan M Melero-Martin,Minglin MaPubMed 34897997
- Oncogenesis 10:832021Cdk5 drives formation of heterogeneous pancreatic neuroendocrine tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAngela M Carter,Nilesh Kumar,Brendon Herring,Chunfeng Tan,Rachael Guenter,Rahul Telange,Wayne Howse,Fabrice Viol,Tyler R McCaw,Hayden H Bickerton,Priyanka Gupta,Frank Gillardon,Eugene A Woltering,Deepti Dhall,John Totenhagen,Ronadip R Banerjee,Elizabeth M Kurian,Sushanth Reddy,Herbert Chen,Joerg Schrader,J Bart Rose,M Shahid Mukhtar,James A BibbPubMed 34862365
- Frontiers in endocrinology 12:7659762021Prolactin-Induced Adaptation in Glucose Homeostasis in Mouse Pregnancy Is Mediated by the Pancreas and Not in the Forebrain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZin Khant Aung,Ilona C Kokay,David R Grattan,Sharon R LadymanPubMed 34867810
- Frontiers in pharmacology 12:7146832021Transgenic Overexpression of Galectin-3 in Pancreatic β Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNemanja Jovicic,Ivica Petrovic,Nada Pejnovic,Biljana Ljujic,Marina Miletic Kovacevic,Sladjana Pavlovic,Ilija Jeftic,Aleksandar Djukic,Ivan Srejovic,Vladimir Jakovljevic,Miodrag L LukicPubMed 34803672
- Nature communications 12:58462021A bioinspired scaffold for rapid oxygenation of cell encapsulation systems.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Hai Wang,Alexander Ulrich Ernst,Duo An,Ashim Kumar Datta,Boris Epel,Mrignayani Kotecha,Minglin MaPubMed 34615868
- Advanced materials (Deerfield Beach, Fla.) 33:e21028522021A Zwitterionic Polyurethane Nanoporous Device with Low Foreign-Body Response for Islet Encapsulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingsheng Liu,Xi Wang,Alan Chiu,Wanjun Liu,Stephanie Fuchs,Bo Wang,Long-Hai Wang,James Flanders,Yidan Zhang,Kai Wang,Juan M Melero-Martin,Minglin MaPubMed 34363254
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Expected | Tested |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.1-1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cat, Chimpanzee, Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1-5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cat, Chimpanzee, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Alternative names
Insulin, INS
Recommended products
Rabbit Polyclonal Insulin antibody. Suitable for IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 45 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
- Biological function summary
Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.
- Pathways
Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.
- Associated diseases and disorders
Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody (ab63820)
ab63820 staining Insulin in Mouse pancreas tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue section underwent formaldehyde fixation and enzymatic epitope retrieval step. The primary antibody was used at 1μg/ml and incubated with the sample for 1 hour. An HRP-conjugated Rabbit HRP polymer system was used undiluted with the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin antibody (ab63820)
IHC image of Insulin staining in a section of formalin-fixed paraffin-embedded normal human normal pancreas* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab63820, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry/ Immunofluorescence - Anti-Insulin antibody (ab63820)
ab63820 staining Insulin in Rin-5F cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab63820 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com