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AB46707

Anti-Insulin antibody [IN-05] - Low endotoxin, Azide free

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(7 Publications)

Mouse Monoclonal Insulin antibody. Carrier free. Suitable for ELISA, IHC-P, RIA, FuncS, ICC/IF and reacts with Pig, Human, Cow samples. Cited in 7 publications. Immunogen corresponding to Full Length Protein corresponding to Pig INS.

View Alternative Names

Insulin, INS

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IN-05

Isotype

IgG1

Carrier free

Yes

Reacts with

Human, Pig, Cow

Applications

ICC/IF, ELISA, FuncS, RIA, IHC-P

applications

Immunogen

Full Length Protein corresponding to Pig INS. The exact immunogen used to generate this antibody is proprietary information.

P01315

Specificity

The antibody blocks binding of insulin to the receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "RIA" : {"fullname" : "Radioimmunoassay", "shortname":"RIA"}, "FuncS" : {"fullname" : "Functional Studies", "shortname":"FuncS"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Blocks the binding of insulin to the receptor.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Cow": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Blocks the binding of insulin to the receptor.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "RIA-species-checked": "guaranteed", "RIA-species-dilution-info": "", "RIA-species-notes": "<p></p>", "FuncS-species-checked": "guaranteed", "FuncS-species-dilution-info": "", "FuncS-species-notes": "<p>Blocks the binding of insulin to the receptor.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. Preserved by filter sterilization.
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:439 PubMed40514650

2025

Nanoscale engineered exosomes for dual delivery of Sirtuin3 and insulin to ignite mitochondrial recovery in myocardial ischemia-reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Yang,Xinyi Yun,Weihan Zheng,Huihui Zhang,Zi Yan,Youyu Chen,Wanting Xue,Siqi Mi,Ziyue Li,Hanxiao Sun,Guozhi Xiao,Zhenning Dai,Shiyu Li,Wenhua Huang

Physiology & behavior 249:113769 PubMed35247443

2022

Effects of nucleus accumbens insulin inactivation on microstructure of licking for glucose and saccharin in male and female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth D Carr,Sydney P Weiner

Nature communications 8:586 PubMed28928405

2017

Nanopore extended field-effect transistor for selective single-molecule biosensing.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Ren,Yanjun Zhang,Binoy Paulose Nadappuram,Bernice Akpinar,David Klenerman,Aleksandar P Ivanov,Joshua B Edel,Yuri Korchev

Physiology & behavior 159:52-63 PubMed26988281

2016

Insulin receptor activation in the nucleus accumbens reflects nutritive value of a recently ingested meal.

Applications

Unspecified application

Species

Unspecified reactive species

C A Woods,Z R Guttman,D Huang,R A Kolaric,A I Rabinowitsch,K T Jones,S Cabeza de Vaca,A Sclafani,K D Carr

Nature communications 6:8543 PubMed26503322

2015

Insulin enhances striatal dopamine release by activating cholinergic interneurons and thereby signals reward.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa A Stouffer,Catherine A Woods,Jyoti C Patel,Christian R Lee,Paul Witkovsky,Li Bao,Robert P Machold,Kymry T Jones,Soledad Cabeza de Vaca,Maarten E A Reith,Kenneth D Carr,Margaret E Rice

International journal of immunopathology and pharm 28:390-402 PubMed26216908

2015

In vitro differentiation of human amniotic epithelial cells into insulin-producing 3D spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

Bernard Okere,Francesco Alviano,Roberta Costa,Daniela Quaglino,Francesca Ricci,Massimo Dominici,Paolo Paolucci,Laura Bonsi,Lorenzo Iughetti

Journal of biomolecular screening 20:536-44 PubMed25381256

2014

Application of the mirrorball high-sensitivity cytometer to multiplexed assays for antibody drug discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth England,Philip Newton,Frances Neal,Lisa Kitching,Caroline Colley,Christine J Rossant
View all publications
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Product promise

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