Rabbit Polyclonal Insulin degrading enzyme / IDE antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IDE.
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- Frontiers in pharmacology 14:12206202023PPAR-gamma agonist pioglitazone recovers mitochondrial quality control in fibroblasts from -deficient patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessia Di Donfrancesco,Christian Berlingieri,Marta Giacomello,Chiara Frascarelli,Ana Paula Magalhaes Rebelo,Laurence A Bindoff,Segel Reeval,Paul Renbaum,Filippo M Santorelli,Giulia Massaro,Carlo Viscomi,Massimo Zeviani,Daniele Ghezzi,Emanuela Bottani,Dario BrunettiPubMed 37576821
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IDE. The exact immunogen used to generate this antibody is proprietary information. Database link P14735
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/20000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/20000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish, Rhesus monkey, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish, Rhesus monkey, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Zebrafish, Rhesus monkey, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:21098034, PubMed:2293021, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:26968463, PubMed:29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed:26394692, PubMed:9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
Alternative names
Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE
Recommended products
Rabbit Polyclonal Insulin degrading enzyme / IDE antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IDE.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IDE. The exact immunogen used to generate this antibody is proprietary information. Database link P14735
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.
- Biological function summary
IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.
- Pathways
IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.
- Associated diseases and disorders
IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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7 product images
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: Insulin degrading enzyme / IDE Knockout HeLa cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution
Lane 1: Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg
Lane 2: Insulin degrading enzyme / IDE knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Insulin degrading enzyme / IDE (green) using ab228720 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
Paraffin-embedded U87 xenograft tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/1000 dilution
Lane 1: Mouse brain extract at 50 µg
Lane 2: Rat brain extract at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/10000 dilution
Lane 1: A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Lane 2: NCI-H1299 (human lung carcinoma cell line) whole cell lysate at 30 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Lane 4: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody (ab228720)
Paraffin-embedded rat brain stem tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.
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