Rabbit Polyclonal Insulin degrading enzyme / IDE antibody. Suitable for WB, IHC-FoFr and reacts with Mouse, Human, Rat samples. Cited in 82 publications.
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- Journal of neuroinflammation 20:2822023Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamkwon Kim,In Gyoung Ju,Seung Ho Jeon,Yeongae Lee,Min-Ji Jung,Min Sung Gee,Jae Seok Cho,Kyung-Soo Inn,Lee Ann Garrett-Sinha,Myung Sook Oh,Jong Kil LeePubMed 38012646
- Molecular psychiatry 28:3966-39812023Mitochondrial hypermetabolism precedes impaired autophagy and synaptic disorganization in App knock-in Alzheimer mouse models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuana Naia,Makoto Shimozawa,Erika Bereczki,Xidan Li,Jianping Liu,Richeng Jiang,Romain Giraud,Nuno Santos Leal,Catarina Moreira Pinho,Erik Berger,Victoria Lim Falk,Giacomo Dentoni,Maria Ankarcrona,Per NilssonPubMed 37907591
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e230342023The day-night differences in cognitive and anxiety-like behaviors of mice after chronic sleep restriction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Zhai,Meng-Mei Yin,Huai-Qing Sun,Xue-Qin Jiang,Yun Liu,Charles Marshall,Ting Wu,Ming XiaoPubMed 37341989
- Cell & bioscience 13:522023A novel rhein-huprine hybrid ameliorates disease-modifying properties in preclinical mice model of Alzheimer's disease exacerbated with high fat diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTriana Espinosa-Jiménez,Amanda Cano,Elena Sánchez-López,Jordi Olloquequi,Jaume Folch,Mònica Bulló,Ester Verdaguer,Carme Auladell,Caterina Pont,Diego Muñoz-Torrero,Antoni Parcerisas,Antoni Camins,Miren EttchetoPubMed 36895036
- Frontiers in immunology 14:11025302023The effect of locus deletion on the progression of Alzheimer's disease-related pathologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHande Karahan,Daniel C Smith,Byungwook Kim,Brianne McCord,Jordan Mantor,Sutha K John,Md Mamun Al-Amin,Luke C Dabin,Jungsu KimPubMed 36895556
- International journal of molecular sciences 24:2023Sleeve Gastrectomy-Induced Weight Loss Increases Insulin Clearance in Obese Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Moreira Soares,Luana Emanuelly Sinhori Lopes,Sandra Lucinei Balbo,Carine Marmentini,Gabriela Alves Bronczek,Mirian Ayumi Kurauti,Maria Lúcia Bonfleur,Licio Augusto Velloso,Everardo Magalhães Carneiro,Antonio Carlos Boschero,José Maria Costa-JúniorPubMed 36675244
- Scientific reports 12:222732022The bile acid TUDCA reduces age-related hyperinsulinemia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucas Zangerolamo,Marina Carvalho,Leticia Barssotti,Gabriela M Soares,Carine Marmentini,Antonio C Boschero,Helena Cristina L BarbosaPubMed 36564463
- Nutrients 14:2022Moringa Oleifera Alleviates Aβ Burden and Improves Synaptic Plasticity and Cognitive Impairments in APP/PS1 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYacoubou Abdoul Razak Mahaman,Jun Feng,Fang Huang,Maibouge Tanko Mahamane Salissou,Jianzhi Wang,Rong Liu,Bin Zhang,Honglian Li,Feiqi Zhu,Xiaochuan WangPubMed 36296969
- Aging and disease 13:1504-15222022Locomotor Hyperactivity in the Early-Stage Alzheimer's Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianqi Wang,Yan Chen,Ying Zou,Yingting Pang,Xiaoxin He,Yali Chen,Yun Liu,Weixi Feng,Yanli Zhang,Qian Li,Jingping Shi,Fengfei Ding,Charles Marshall,Junying Gao,Ming XiaoPubMed 36186142
- Journal of neurochemistry 163:26-392022Endoplasmic reticulum stress induces Alzheimer disease-like phenotypes in the neuron derived from the induced pluripotent stem cell with D678H mutation on amyloid precursor protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTania Devina,Yu-Hui Wong,Chiao-Wan Hsiao,Yu-Jui Li,Cheng-Chang Lien,Irene Han-Juo ChengPubMed 35943292
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-FoFr | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Rat | Expected | Tested |
| Cat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat | Dilution info - | Notes - |
IHC-FoFr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat | Dilution info - | Notes - |
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Target data
Function
Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:21098034, PubMed:2293021, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:26968463, PubMed:29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed:26394692, PubMed:9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
Alternative names
Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE
Recommended products
Rabbit Polyclonal Insulin degrading enzyme / IDE antibody. Suitable for WB, IHC-FoFr and reacts with Mouse, Human, Rat samples. Cited in 82 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab32216 are tested in WB. Previous batches were additionally validated in IHC-FoFr. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab133561.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.
- Biological function summary
IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.
- Pathways
IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.
- Associated diseases and disorders
IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Insulin degrading enzyme / IDE knockout HAP1 cell lysate (20 μg)
Lane 3: K562 cell lysate (20 μg)
Lane 4: HepG2 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32216 observed at 120 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab32216 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells. No band was observed when Insulin degrading enzyme / IDE knockout samples were examined. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. ab32216 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Predicted band size: 118 kDa
This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Immunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective.
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)
All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/mL
Lane 1: Mouse Brain at 20 µg/mL
Lane 2: Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3: Mouse Hippocampus Lysate at 20 µg
Lane 4: Rat Hippocampus Lysate at 20 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
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