AB32216

Anti-Insulin degrading enzyme / IDE antibody

Name: Anti-Insulin degrading enzyme / IDE antibody KO tested (ab32216) | Abcam
Brand: Abcam Limited
SKU: AB32216
Rating: 4 (9 reviews)

KO Validated
What is this?

(9 Reviews)

(97 Publications)

Rabbit Polyclonal Insulin degrading enzyme / IDE antibody. Suitable for WB, IHC-FoFr and reacts with Mouse, Human, Rat samples. Cited in 97 publications.

View Alternative Names

Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE

3 Images

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

IHC-FoFr

Collaborator

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

Immunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details : Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

This image is courtesy of Sophie Pezet, CNRS, Paris, France

Lab

Western blot - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Insulin degrading enzyme / IDE knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab32216 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32216 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells. No band was observed when Insulin degrading enzyme / IDE knockout samples were examined. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. ab32216 and ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216)

Predicted band size: 118 kDa

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Project

Western blot - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/mL

Lane 1:

Mouse Brain at 20 µg/mL

Lane 2:

Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg

Lane 3:

Mouse Hippocampus Lysate at 20 µg

Lane 4:

Rat Hippocampus Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-FoFr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of our polyclonal antibody, ab32216 are tested in WB. Previous batches were additionally validated in IHC-FoFr. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab133561.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "testedAndGuaranteed", "IHCFoFr-species-dilution-info": "1/100", "IHCFoFr-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "predicted", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.

Biological function summary

IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.

Pathways

IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.

IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed : 10684867, PubMed : 17051221, PubMed : 17613531, PubMed : 18986166, PubMed : 19321446, PubMed : 21098034, PubMed : 2293021, PubMed : 23922390, PubMed : 24847884, PubMed : 26394692, PubMed : 26968463, PubMed : 29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed : 23922390, PubMed : 26394692, PubMed : 29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed : 26394692, PubMed : 9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed : 21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed : 21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

See full target information IDE

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Publications (97)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70292 PubMed40457749

2025

Acute experimental colitis in 5xFAD Alzheimer's disease mice leads to enhanced monocyte infiltration into the brain accompanied by reduced β-amyloid deposition.

Applications

Unspecified application

Species

Unspecified reactive species

Nanda K Navalpur Shanmugam,Frank Zamudio,Deepak K Vijaya Kumar,Kianna A Barrett,Ryan VanDoren,Meng Chen,Olivia M Barr,Sara Watson,Chih-Chung Jerry Lin,William A Eimer,Mehdi Jorfi,Se Hoon Choi,Robert D Moir,Rudolph E Tanzi

The Journal of neuroscience : the official journal of the Society for Neuroscience 45: PubMed40300834

2025

The Role of Neprilysin and Insulin-Degrading Enzyme in the Etiology of Sporadic Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Morito,Shoko Hashimoto,Risa Takamura,Naoto Watamura,Naomasa Kakiya,Ryo Fujioka,Naomi Mihara,Misaki Sekiguchi,Kaori Watanabe-Iwata,Naoko Kamano,Mohan Qi,Yukio Matsuba,Satoshi Tsubuki,Takashi Saito,Nobuhisa Iwata,Hiroki Sasaguri,Takaomi C Saido

Scientific reports 14:27614 PubMed39528509

2024

Primate-specific 82-kDa choline acetyltransferase attenuates progression of Alzheimer's disease-like pathology in the APP knock-in mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Hadir E AlQot,Rebecca Jane Rylett

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39496485

2024

The Icelandic Mutation (APP-A673T) Is Protective against Amyloid Pathology In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Sho Shimohama,Ryo Fujioka,Naomi Mihira,Misaki Sekiguchi,Luca Sartori,Daisuke Joho,Takashi Saito,Takaomi C Saido,Jin Nakahara,Tomohito Hino,Atsushi Hoshino,Hiroki Sasaguri

British journal of pharmacology 181:3610-3626 PubMed38812293

2024

Insulin-degrading enzyme inhibition increases the unfolded protein response and favours lipid accumulation in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Marine Andres,Nathalie Hennuyer,Khamis Zibar,Marie Bicharel-Leconte,Isabelle Duplan,Emmanuelle Enée,Emmanuelle Vallez,Adrien Herledan,Anne Loyens,Bart Staels,Benoit Deprez,Peter van Endert,Rebecca Deprez-Poulain,Steve Lancel

Journal of pharmaceutical analysis 14:348-370 PubMed38618251

2024

Altered synaptic currents, mitophagy, mitochondrial dynamics in Alzheimer's disease models and therapeutic potential of Dengzhan Shengmai capsules intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Zhao,Dongfeng Wei,Qinghua Long,Qingjie Chen,Fushun Wang,Linlin Chen,Zefei Li,Tong Li,Tao Ma,Wei Liu,Linshuang Wang,Caishui Yang,Xiaxia Zhang,Ping Wang,Zhanjun Zhang

Journal of neuroinflammation 20:282 PubMed38012646

2023

Inhibition of microfold cells ameliorates early pathological phenotypes by modulating microglial functions in Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Namkwon Kim,In Gyoung Ju,Seung Ho Jeon,Yeongae Lee,Min-Ji Jung,Min Sung Gee,Jae Seok Cho,Kyung-Soo Inn,Lee Ann Garrett-Sinha,Myung Sook Oh,Jong Kil Lee

Molecular psychiatry 28:3966-3981 PubMed37907591

2023

Mitochondrial hypermetabolism precedes impaired autophagy and synaptic disorganization in App knock-in Alzheimer mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Luana Naia,Makoto Shimozawa,Erika Bereczki,Xidan Li,Jianping Liu,Richeng Jiang,Romain Giraud,Nuno Santos Leal,Catarina Moreira Pinho,Erik Berger,Victoria Lim Falk,Giacomo Dentoni,Maria Ankarcrona,Per Nilsson

Neuron 111:3619-3633.e8 PubMed37689059

2023

Irisin reduces amyloid-β by inducing the release of neprilysin from astrocytes following downregulation of ERK-STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Eunhee Kim,Hyeonwoo Kim,Mark P Jedrychowski,Grisilda Bakiasi,Joseph Park,Jane Kruskop,Younjung Choi,Sang Su Kwak,Luisa Quinti,Doo Yeon Kim,Christiane D Wrann,Bruce M Spiegelman,Rudolph E Tanzi,Se Hoon Choi

Frontiers in neuroimaging 1:903531 PubMed37555169

2023

Synergistic photobiomodulation with 808-nm and 1064-nm lasers to reduce the β-amyloid neurotoxicity in the Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Renlong Zhang,Ting Zhou,Soham Samanta,Ziyi Luo,Shaowei Li,Hao Xu,Junle Qu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Insulin degrading enzyme / IDE antibody

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

Western blot - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

Western blot - Anti-Insulin degrading enzyme / IDE antibody (AB32216)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (97)

Product promise