Skip to main content

Rabbit Recombinant Monoclonal Insulin degrading enzyme / IDE antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 17 publications.


Images

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561), expandable thumbnail
  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561), expandable thumbnail
  • OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IFIHC-Fr
Human
Tested
Not recommended
Not recommended
Tested
Not recommended
Expected
Mouse
Expected
Not recommended
Not recommended
Expected
Not recommended
Tested
Rat
Predicted
Not recommended
Not recommended
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:21098034, PubMed:2293021, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:26968463, PubMed:29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed:26394692, PubMed:9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

Alternative names

Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE

Recommended products

Rabbit Recombinant Monoclonal Insulin degrading enzyme / IDE antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 17 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6098(2)
Purification technique
Affinity purification Protein A
Dissociation constant
2.31 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.

Biological function summary

IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.

Pathways

IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.

Associated diseases and disorders

IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561), expandable thumbnail

    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: IDE knockout HAP1 cell lysate (20 μg)
    Lane 3: K562 cell lysate (20 μg)
    Lane 4: HepG2 cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    Predicted band size: 118 kDa

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561), expandable thumbnail

    Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    All lanes: Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: A375 cell lysate at 10 µg

    Lane 4: K562 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 118 kDa

    Observed band size: 120 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561), expandable thumbnail

    OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Frozen sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

    Immunohistochemical analysis of 4% PFA permeabilized frozen mouse kidney (perfused fixed) tissue labelling Insulin degrading enzyme / IDE with ab133561 at /100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.

    Confocal image showing positive staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab133561 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com