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AB133561

Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

4

(5 Reviews)

|

(21 Publications)

Rabbit Recombinant Monoclonal Insulin degrading enzyme / IDE antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Human, Mouse samples. Cited in 21 publications.

View Alternative Names

Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)

Immunohistochemical analysis of 4% PFA permeabilized frozen mouse kidney (perfused fixed) tissue labelling Insulin degrading enzyme / IDE with ab133561 at /100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

Confocal image showing positive staining on mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab133561 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)
  • WB

Supplier Data

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : IDE knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)

Predicted band size: 118 kDa

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Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)
  • WB

Unknown

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)

All lanes:

Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A375 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 118 kDa

Observed band size: 120 kDa

false

OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (AB133561)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6098(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-Fr, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.
Biological function summary

IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.

Pathways

IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.

IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed : 10684867, PubMed : 17051221, PubMed : 17613531, PubMed : 18986166, PubMed : 19321446, PubMed : 21098034, PubMed : 2293021, PubMed : 23922390, PubMed : 24847884, PubMed : 26394692, PubMed : 26968463, PubMed : 29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed : 23922390, PubMed : 26394692, PubMed : 29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed : 26394692, PubMed : 9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed : 21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed : 21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.. (Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
See full target information IDE
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70580 PubMed40843775

2025

Ms4a4a deficiency ameliorates plaque pathology in a mouse model of amyloid accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Emma P Danhash,Anthony C Verbeck,Daniel Western,Andrea S Díaz-Pacheco,Grant Galasso,Shih-Feng You,Guangming Huang,Emma Starr,Nadia Miller,Collin J Nadarajah,Savannah Tiemann Powles,Erik S Musiek,Jasmin Herz,Abhirami K Iyer,John Cirrito,Carlos Cruchaga,Celeste M Karch

CNS neuroscience & therapeutics 30:e70127 PubMed39592913

2024

Farnesylthiosalicylic Acid Through Inhibition of Galectin-3 Improves Neuroinflammation in Alzheimer Disease via Multiple Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Qiu,Cui Li,Xiaoli Zhao,Mengting Yang,Shushu Ding,Haiying Liang,Tingting Chen

Diabetic medicine : a journal of the British Diabetic Association 41:e15319 PubMed38711201

2024

Berberine attenuates obesity-induced insulin resistance by inhibiting miR-27a secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Junda Du,Yu Zhu,Xuehan Yang,Xinru Geng,Yang Xu,Meishuang Zhang,Ming Zhang

Journal of Alzheimer's disease : JAD 82:1315-1332 PubMed34151811

2021

Blockage of Fc Gamma Receptors Alleviates Neuronal and Microglial Toxicity Induced by Palmitic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Phansa Phitthayaphong,Sirinart Kumfu,Nipon Chattipakorn,Siriporn C Chattipakorn

Aging 13:15285-15306 PubMed34086603

2021

Mesenchymal stem cells-derived extracellular vesicles ameliorate Alzheimer's disease in rat models via the microRNA-29c-3p/BACE1 axis and the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Sha,Xueli Shen,Yunpeng Cao,Le Qu

Frontiers in aging neuroscience 13:650103 PubMed33776747

2021

Intranasal Transplantation of Human Neural Stem Cells Ameliorates Alzheimer's Disease-Like Pathology in a Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Hong Lu,Wen-Li Ji,Hong Chen,Yan-Yun Sun,Xiu-Yun Zhao,Fen Wang,Yi Shi,Yan-Ning Hu,Bo-Xiang Liu,Jing-Wen Wu,De-En Xu,Jia-Wei Zheng,Chun-Feng Liu,Quan-Hong Ma

Hepatology communications 5:446-460 PubMed33681678

2021

High-Fat Diet Modulates Hepatic Amyloid β and Cerebrosterol Metabolism in the Triple Transgenic Mouse Model of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina R Bosoi,Milène Vandal,Marine Tournissac,Manon Leclerc,Hortense Fanet,Patricia L Mitchell,Mélanie Verreault,Jocelyn Trottier,Jessica Virgili,Cynthia Tremblay,H Robert Lippman,Jasmohan S Bajaj,Olivier Barbier,André Marette,Frédéric Calon

Neural regeneration research 16:2078-2085 PubMed33642397

2021

Combined treatment with valproic acid and estrogen has neuroprotective effects in ovariectomized mice with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Zhen Li,Yuan-Jie Liu,Wei Zhang,Shi-Fang Luo,Xin Zhou,Gui-Qiong He

Molecular medicine reports 23: PubMed33604684

2021

CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating β‑amyloid metabolism‑associated enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Jiang,Fengnan Niu,Yan Zheng,Yun Xu

Alzheimer's research & therapy 13:7 PubMed33397436

2021

Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aβ clearance in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Su,Tian Li,Pei-Feng He,Xue-Chun Lu,Qi Yu,Qi-Chao Gao,Zhao-Jun Wang,Mei-Na Wu,Dan Yang,Jin-Shun Qi
View all publications
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