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Mouse Monoclonal Insulin degrading enzyme / IDE antibody. Carrier free. Suitable for WB and reacts with Mouse, African green monkey, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Insulin-degrading enzyme aa 800 to C-terminus.

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Images

Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (AB273669), expandable thumbnail
  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (AB273669), expandable thumbnail
  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (AB273669), expandable thumbnail

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.3
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human Insulin-degrading enzyme aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P14735

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
African green monkey
Tested

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

African green monkey

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Target data

Function

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:21098034, PubMed:2293021, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:26968463, PubMed:29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed:26394692, PubMed:9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.(Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

Alternative names

Recommended products

Mouse Monoclonal Insulin degrading enzyme / IDE antibody. Carrier free. Suitable for WB and reacts with Mouse, African green monkey, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Insulin-degrading enzyme aa 800 to C-terminus.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human Insulin-degrading enzyme aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P14735
Carrier free

Yes

Clone number

OTI4E5

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.

Biological function summary

IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.

Pathways

IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.

Associated diseases and disorders

IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.

Product promise

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3 product images

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669), expandable thumbnail

    Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    All lanes: Same antibody clone in a different buffer formulation at 1/500 dilution

    Lane 1: Wild type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

    Lane 2: IDE knock out HeLa cell lysate at 10 µg

    Predicted band size: 118 kDa

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669), expandable thumbnail

    Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    All lanes: Same antibody clone in a different buffer formulation at 1/500 dilution

    Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg

    Lane 2: SVT2 (Mouse cell line) cell extract at 35 µg

    Lane 3: A549 (Human lung carcinoma cell line) cell extract at 35 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

    Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg

    Predicted band size: 118 kDa

  • Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669), expandable thumbnail

    Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    All lanes: Same antibody clone in a different buffer formulation at 1/500 dilution

    Lane 1: HEK-293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg

    Lane 2: HEK-293T cells transfected with pCMV6-ENTRY IDE cDNA at 5 µg

    Predicted band size: 118 kDa, 81 kDa

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Product protocols

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