Mouse Monoclonal Insulin degrading enzyme / IDE antibody. Carrier free. Suitable for WB and reacts with Mouse, African green monkey, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Insulin-degrading enzyme aa 800 to C-terminus.
IgG2b
Mouse
pH: 7.3
Constituents: PBS
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
African green monkey | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species African green monkey | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and other peptides, and thereby plays a role in intercellular peptide signaling (PubMed:10684867, PubMed:17051221, PubMed:17613531, PubMed:18986166, PubMed:19321446, PubMed:21098034, PubMed:2293021, PubMed:23922390, PubMed:24847884, PubMed:26394692, PubMed:26968463, PubMed:29596046). Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin (PubMed:23922390, PubMed:26394692, PubMed:29596046). Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable) (PubMed:26394692, PubMed:9830016). Degrades the natriuretic peptides ANP, BNP and CNP, inactivating their ability to raise intracellular cGMP (PubMed:21098034). Also degrades an aberrant frameshifted 40-residue form of NPPA (fsNPPA) which is associated with familial atrial fibrillation in heterozygous patients (PubMed:21098034). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.(Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).
Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulysin, Insulinase, IDE
Mouse Monoclonal Insulin degrading enzyme / IDE antibody. Carrier free. Suitable for WB and reacts with Mouse, African green monkey, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Insulin-degrading enzyme aa 800 to C-terminus.
IgG2b
Mouse
pH: 7.3
Constituents: PBS
Liquid
Monoclonal
Yes
OTI4E5
Affinity purification Protein A/G
Purified from TCS.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Insulin degrading enzyme (IDE) also known as insulinase is a zinc metalloprotease involved in the breakdown of small proteins including insulin. IDE has a molecular weight of approximately 110 kDa. It works by cleaving the peptide bonds of its substrate proteins therefore decreasing their molecular integrity. IDE is expressed in several tissues including the liver muscle and kidney where it plays a significant role in regulating metabolic processes. This protein can be found both within cells and in the extracellular space.
IDE manages the levels of insulin and other peptides by degrading them preventing accumulation and maintaining homeostasis. It is not part of a complex but it acts individually in cellular environments to modulate the concentration of its substrates. IDE is important for controlling insulin availability and turnover which impacts glucose metabolism. By influencing the degradation of insulin IDE aids in balancing metabolic demands with insulin availability.
IDE plays a vital role in insulin signaling and glucose metabolic processes. It is directly involved in the insulin signaling pathway by regulating insulin levels which consequently affects cellular responses to insulin. IDE connects with several proteins associated with these pathways including insulin receptor and glucose transporters ensuring proper cell signaling and metabolic functions. By modulating insulin levels IDE helps optimize glucose uptake and storage.
IDE has a relevant connection to Alzheimer's disease and type 2 diabetes. Its role in insulin degradation links it to type 2 diabetes where dysregulation of insulin levels can exacerbate the disease. IDE is also associated with Alzheimer's disease since it degrades amyloid-beta peptides. Any malfunction or altered expression of IDE can lead to accumulation of these peptides contributing to Alzheimer's pathology. In the context of these diseases IDE interacts with amyloid-beta precursor protein and components of insulin signaling pathways highlighting its significance in maintaining health and preventing disease progression.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
All lanes: Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1: Wild type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg
Lane 2: IDE knock out HeLa cell lysate at 10 µg
Predicted band size: 118 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
All lanes: Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1: HepG2 (Human liver hepatocellular carcinoma cell line) cell extract at 35 µg
Lane 2: SVT2 (Mouse cell line) cell extract at 35 µg
Lane 3: A549 (Human lung carcinoma cell line) cell extract at 35 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg
Lane 5: COS-7 (African green monkey kidney fibroblast-like cell line) cell extract at 35 µg
Predicted band size: 118 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
All lanes: Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1: HEK-293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg
Lane 2: HEK-293T cells transfected with pCMV6-ENTRY IDE cDNA at 5 µg
Predicted band size: 118 kDa, 81 kDa
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