AB8305

Anti-Insulin + Proinsulin antibody [D3E7]

Name: Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) | Abcam
Brand: Abcam Limited
SKU: AB8305
Price: 420 USD
Availability: InStock

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(2 Publications)

Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) is a mouse monoclonal antibody detecting Insulin + Proinsulin in IHC-Fr, ELISA. Suitable for Cow, Human, Mouse, Pig, Rat.

- Trusted since 2002

View Alternative Names

Insulin, INS

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

D3E7

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Cow, Human, Pig

Applications

ELISA, IHC-Fr

applications

Specificity

Kd for this antibody is 6.3 x 10^-8M.This antibody is specific for both insulin and proinsulin

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Insulin + Proinsulin antibody [D3E7] (ab8305) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr), ELISA in Cow, Human, Mouse, Pig, Rat samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin often referred to as 'insulin' is a peptide hormone with a mass of approximately 5.8 kDa generated by the beta cells in the pancreas. Its precursor proinsulin is synthesized as a larger molecule with a mass of about 9 kDa. Both insulin and proinsulin are primarily expressed in the pancreatic islets of Langerhans. Proinsulin undergoes enzymatic cleavage to form mature insulin and C-peptide. Insulin plays an essential role in glucose metabolism by regulating cellular uptake of glucose.

Biological function summary

Insulin and proinsulin are indispensable for maintaining glucose homeostasis in the body. Insulin binds to its receptor a receptor tyrosine kinase on target cells like muscle and adipose tissue to facilitate glucose uptake. Proinsulin also has a lesser-known role in insulin regulation and folding. It mostly exists independently rather than being part of a complex with insulin but precursor and mature insulin forms can transiently appear together during synthesis and secretion.

Pathways

Insulin function fits significantly into the signaling pathways such as the insulin signaling pathway and the PI3K/AKT pathway. These pathways importantly involve glucose transporters like GLUT4 and proteins such as IRS-1 that mediate insulin's effects on glucose and lipid metabolism. Insulin signals lead to the translocation of GLUT4 to the plasma membrane enhancing glucose uptake. Therefore insulin is closely related to these critical metabolic pathways.

Dysfunctions in insulin and proinsulin levels or actions are linked to conditions like diabetes mellitus and metabolic syndrome. In diabetes insulin resistance or deficiency leads to uncontrolled blood glucose levels highlighting the importance of insulin in glucose regulation. The link between insulin and obesity often involves dysregulation in insulin signaling pathways contributing to metabolic complications. Understanding these processes and their diseases is vital for developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information INS

Additional targets

Proinsulin

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36142497

2022

Recombinant Reg3α Prevents Islet β-Cell Apoptosis and Promotes β-Cell Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Luting Yu,Liang Li,Junli Liu,Hao Sun,Xiang Li,Hanyu Xiao,Martin Omondi Alfred,Min Wang,Xuri Wu,Yan Gao,Chen Luo

Gastroenterology 141:1451-62, 1462.e1-6 PubMed21763240

2011

Duct cells contribute to regeneration of endocrine and acinar cells following pancreatic damage in adult mice.

Applications

IHC-Fr

Species

Mouse

Angela Criscimanna,Julie A Speicher,Golbahar Houshmand,Chiyo Shiota,Krishna Prasadan,Baoan Ji,Craig D Logsdon,George K Gittes,Farzad Esni

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Insulin + Proinsulin antibody [D3E7]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

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