AB8304

Anti-Insulin + Proinsulin antibody [D6C4]

Name: Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) | Abcam
Brand: Abcam Limited
SKU: AB8304
Price: 490 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(34 Publications)

Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) is a mouse monoclonal antibody detecting Insulin + Proinsulin in ELISA. Suitable for Cow, Human, Mouse, Pig, Rat.

- Over 30 publications
- Trusted since 2002

View Alternative Names

Insulin, INS

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

D6C4

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Pig, Mouse, Rat, Cow, Human

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Specific for insulin and proinsulin.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "10 g/mL", "ELISA-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "10 g/mL", "ELISA-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "10 g/mL", "ELISA-species-notes": "" }, "Cow": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "10 g/mL", "ELISA-species-notes": "" }, "Pig": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "10 g/mL", "ELISA-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) is a mouse monoclonal antibody and is validated for use in ELISA in Cow, Human, Mouse, Pig, Rat samples.

Trusted by the scientific community
Anti-Insulin + Proinsulin [D6C4] (ab8304) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Insulin + Proinsulin [D6C4] (ab8304) has over 5 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin often referred to as 'insulin' is a peptide hormone with a mass of approximately 5.8 kDa generated by the beta cells in the pancreas. Its precursor proinsulin is synthesized as a larger molecule with a mass of about 9 kDa. Both insulin and proinsulin are primarily expressed in the pancreatic islets of Langerhans. Proinsulin undergoes enzymatic cleavage to form mature insulin and C-peptide. Insulin plays an essential role in glucose metabolism by regulating cellular uptake of glucose.

Biological function summary

Insulin and proinsulin are indispensable for maintaining glucose homeostasis in the body. Insulin binds to its receptor a receptor tyrosine kinase on target cells like muscle and adipose tissue to facilitate glucose uptake. Proinsulin also has a lesser-known role in insulin regulation and folding. It mostly exists independently rather than being part of a complex with insulin but precursor and mature insulin forms can transiently appear together during synthesis and secretion.

Pathways

Insulin function fits significantly into the signaling pathways such as the insulin signaling pathway and the PI3K/AKT pathway. These pathways importantly involve glucose transporters like GLUT4 and proteins such as IRS-1 that mediate insulin's effects on glucose and lipid metabolism. Insulin signals lead to the translocation of GLUT4 to the plasma membrane enhancing glucose uptake. Therefore insulin is closely related to these critical metabolic pathways.

Dysfunctions in insulin and proinsulin levels or actions are linked to conditions like diabetes mellitus and metabolic syndrome. In diabetes insulin resistance or deficiency leads to uncontrolled blood glucose levels highlighting the importance of insulin in glucose regulation. The link between insulin and obesity often involves dysregulation in insulin signaling pathways contributing to metabolic complications. Understanding these processes and their diseases is vital for developing therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information Insulin

style

left-column

Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Drug delivery and translational research 13:1699-1715 PubMed36587110

2023

Functionalized zein nanoparticles targeting neonatal Fc receptor to enhance lung absorption of peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Hameedat,Soraia Pinto,Joana Marques,Sofia Dias,Bruno Sarmento

The Journal of physiology 600:1611-1630 PubMed35128667

2022

Development and characterisation of a rat model that exhibits both metabolic dysfunction and neurodegeneration seen in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Southam,Chantal de Sousa,Abraham Daniel,Bruce V Taylor,Lisa Foa,Dino Premilovac

Atherosclerosis 326:47-55 PubMed33933263

2021

Effects of proprotein convertase subtilisin kexin type 9 modulation in human pancreatic beta cells function.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Ramin-Mangata,Aurélie Thedrez,Brice Nativel,Nicolas Diotel,Valentin Blanchard,Matthieu Wargny,Audrey Aguesse,Stéphanie Billon-Crossouard,Cécile Vindis,Cédric Le May,Philippe Hulin,Mathieu Armanet,Valery Gmyr,François Pattou,Mikaël Croyal,Olivier Meilhac,Estelle Nobécourt,Bertrand Cariou,Gilles Lambert

Diabetes 70:449-463 PubMed33199363

2020

Retinol-Binding Protein 4 Activates STRA6, Provoking Pancreatic β-Cell Dysfunction in Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Huang,Xinxiu Bai,Xueyan Li,Xiaohui Wang,Lina Zhao

Transplantation direct 6:e620 PubMed33134496

2020

Prevascularization-free Primary Subcutaneous Transplantation of Xenogeneic Islets Coencapsulated With Hepatocyte Growth Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Sin-Yu Yang,Kai-Chiang Yang,Shoichiro Sumi

Tissue & cell 67:101439 PubMed32979709

2020

Differentiation of rat pancreatic duct stem cells into insulin-secreting islet-like cell clusters through BMP7 inducement.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Waseem Ghani,Liu Bin,Yang Jie,Zhao Yi,Wu Jiang,Li Ye,Lang Guan Cun,Muhammad Waseem Birmani,Zhao Zhuangzhi,An Lilong,Xiao Mei

Molecular therapy. Methods & clinical development 18:856-868 PubMed32953935

2020

High-Resolution Histological Landscape of AAV DNA Distribution in Cellular Compartments and Tissues following Local and Systemic Injection.

Applications

Unspecified application

Species

Unspecified reactive species

Junling Zhao,Yongping Yue,Aman Patel,Lakmini Wasala,Jacob F Karp,Keqing Zhang,Dongsheng Duan,Yi Lai

PloS one 15:e0233502 PubMed32433667

2020

ERp29 as a regulator of Insulin biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Viviano,Margaret Brecker,Christine Ferrara-Cook,Laurence Suaud,Ronald C Rubenstein

BMC complementary medicine and therapies 20:136 PubMed32375753

2020

Administration of mulberry leaves maintains pancreatic β-cell mass in obese/type 2 diabetes mellitus mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Patlada Suthamwong,Manabu Minami,Toshiaki Okada,Nonomi Shiwaku,Mai Uesugi,Masayuki Yokode,Kaeko Kamei

Molecular and cellular biology 39: PubMed31481450

2019

1-Palmitoyl-2-Linoleoyl-3-Acetyl--Glycerol Attenuates Streptozotocin-Induced Pancreatic Beta Cell Damage by Promoting Glucose Transporter 2 Endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jimin Kim,Joo Heon Kim,Ki-Young Sohn,Sun Young Yoon,Jae Wha Kim

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Insulin + Proinsulin antibody [D6C4]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise