AB8304

Anti-Insulin + Proinsulin antibody [D6C4]

Name: Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) | Abcam
Brand: Abcam Limited
SKU: AB8304
Price: 490 USD
Availability: InStock
Rating: 4 (6 reviews)

(6 Reviews)

(41 Publications)

Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) is a mouse monoclonal antibody detecting Insulin + Proinsulin in ELISA. Suitable for Cow, Human, Mouse, Pig, Rat.

- Over 30 publications
- Trusted since 2002

View Alternative Names

Insulin, INS

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

D6C4

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Pig, Mouse, Rat, Cow, Human

Applications

ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human Insulin. The exact immunogen used to generate this antibody is proprietary information.

P01308

Specificity

Specific for insulin and proinsulin.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Insulin + Proinsulin antibody [D6C4] (ab8304) is a mouse monoclonal antibody and is validated for use in ELISA in Cow, Human, Mouse, Pig, Rat samples.

Trusted by the scientific community
Anti-Insulin + Proinsulin [D6C4] (ab8304) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Insulin + Proinsulin [D6C4] (ab8304) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin often referred to as 'insulin' is a peptide hormone with a mass of approximately 5.8 kDa generated by the beta cells in the pancreas. Its precursor proinsulin is synthesized as a larger molecule with a mass of about 9 kDa. Both insulin and proinsulin are primarily expressed in the pancreatic islets of Langerhans. Proinsulin undergoes enzymatic cleavage to form mature insulin and C-peptide. Insulin plays an essential role in glucose metabolism by regulating cellular uptake of glucose.

Biological function summary

Insulin and proinsulin are indispensable for maintaining glucose homeostasis in the body. Insulin binds to its receptor a receptor tyrosine kinase on target cells like muscle and adipose tissue to facilitate glucose uptake. Proinsulin also has a lesser-known role in insulin regulation and folding. It mostly exists independently rather than being part of a complex with insulin but precursor and mature insulin forms can transiently appear together during synthesis and secretion.

Pathways

Insulin function fits significantly into the signaling pathways such as the insulin signaling pathway and the PI3K/AKT pathway. These pathways importantly involve glucose transporters like GLUT4 and proteins such as IRS-1 that mediate insulin's effects on glucose and lipid metabolism. Insulin signals lead to the translocation of GLUT4 to the plasma membrane enhancing glucose uptake. Therefore insulin is closely related to these critical metabolic pathways.

Dysfunctions in insulin and proinsulin levels or actions are linked to conditions like diabetes mellitus and metabolic syndrome. In diabetes insulin resistance or deficiency leads to uncontrolled blood glucose levels highlighting the importance of insulin in glucose regulation. The link between insulin and obesity often involves dysregulation in insulin signaling pathways contributing to metabolic complications. Understanding these processes and their diseases is vital for developing therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information Insulin

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Anais da Academia Brasileira de Ciencias 97:e20240807 PubMed40136201

2025

Maternal diabetes on fetal endocrine pancreas development in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Isabela L Iessi,Bruna Dallaqua,Yuri K Sinzato,Franciane Q Gallego,Jens H Nielsen,Gustavo T Volpato,José E Corrente,Débora C Damasceno

Scientific reports 15:8812 PubMed40087321

2025

Controlled induction of type 2 diabetes in mice using high fat diet and osmotic-mini pump infused streptozotocin.

Applications

Unspecified application

Species

Unspecified reactive species

Emily H Attrill,Oscar Scharapow,Sathya Perera,Sophie Mayne,Nicole Sumargo,Renee M Ross,Stephen M Richards,Brad A Sutherland,Dino Premilovac

Endocrinology 166: PubMed39844660

2025

Interaction of B0AT1 Deficiency and Diet on Metabolic Function and Diabetes Incidence in Male Nonobese Diabetic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew F Waters,Viviane Delghingaro-Augusto,Muhammad Shamoon,Kiran Javed,Gaetan Burgio,Jane E Dahlstrom,Stefan Bröer,Christopher J Nolan

The Journal of biological chemistry 300:107425 PubMed38823639

2024

A crucial role of adenosine deaminase in regulating gluconeogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Ding,Wenhao Ge,Xiaogang Xu,Xiaodong Xu,Qi Sun,Xi Xu,Jianfa Zhang

Heliyon 10:e31049 PubMed38803977

2024

Effect of transgenerational diabetes via maternal lineage in female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Franciane Quintanilha Gallego,Vinícius Soares Barco,Yuri Karen Sinzato,Verônyca Gonçalves Paula,Maysa Rocha de Souza,Larissa Lopes da Cruz,Sayon Roy,José Eduardo Corrente,Débora Cristina Damasceno

ACS pharmacology & translational science 7:1650-1663 PubMed38751616

2024

GLP-1 Analogue-Loaded Glucose-Responsive Nanoparticles as Allies of Stem Cell Therapies for the Treatment of Type I Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Joana Moreira Marques,Rute Nunes,Ana Margarida Carvalho,Helena Florindo,Domingos Ferreira,Bruno Sarmento

Drug delivery and translational research 13:1699-1715 PubMed36587110

2023

Functionalized zein nanoparticles targeting neonatal Fc receptor to enhance lung absorption of peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Hameedat,Soraia Pinto,Joana Marques,Sofia Dias,Bruno Sarmento

The Journal of physiology 600:1611-1630 PubMed35128667

2022

Development and characterisation of a rat model that exhibits both metabolic dysfunction and neurodegeneration seen in type 2 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Southam,Chantal de Sousa,Abraham Daniel,Bruce V Taylor,Lisa Foa,Dino Premilovac

Atherosclerosis 326:47-55 PubMed33933263

2021

Effects of proprotein convertase subtilisin kexin type 9 modulation in human pancreatic beta cells function.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphane Ramin-Mangata,Aurélie Thedrez,Brice Nativel,Nicolas Diotel,Valentin Blanchard,Matthieu Wargny,Audrey Aguesse,Stéphanie Billon-Crossouard,Cécile Vindis,Cédric Le May,Philippe Hulin,Mathieu Armanet,Valery Gmyr,François Pattou,Mikaël Croyal,Olivier Meilhac,Estelle Nobécourt,Bertrand Cariou,Gilles Lambert

mAbs 13:1864084 PubMed33382949

2021

Combining phage display with SMRTbell next-generation sequencing for the rapid discovery of functional scFv fragments.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Nannini,Lenart Senicar,Farhaan Parekh,Khai J Kong,Alexander Kinna,Reyisa Bughda,James Sillibourne,Xihao Hu,Biao Ma,Yuchen Bai,Mathieu Ferrari,Martin A Pule,Shimobi C Onuoha

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Insulin + Proinsulin antibody [D6C4]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (41)

Product promise