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Rabbit Recombinant Monoclonal Insulin Receptor phospho Y1361 + Y1361 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689), expandable thumbnail
  • Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (AB283689), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. In adipocytes, inhibits lipolysis (By similarity).

Alternative names

Insulin receptor, IR, INSR

Recommended products

Rabbit Recombinant Monoclonal Insulin Receptor phospho Y1361 + Y1361 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23962-157

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Insulin Receptor alpha (INSTA) sometimes called Insulin Receptor Subunit alpha is an integral component of the insulin receptor complex. It is an important site for insulin binding and exhibits a molecular weight (MW) of approximately 157 kDa. The receptor commonly distributes on the surface of cells with high expression in muscle cells liver cells and adipose tissue. Insulin receptor antibodies can target this protein for various studies including Western blot analyses. The protein structure itself comprises two alpha subunits alongside two beta subunits which together facilitate insulin's physiological effects.

Biological function summary

INSTA plays a critical role in glucose uptake and regulation of energy metabolism. It is an extracellular receptor involved in the formation of a tetrameric complex when interacting with insulin triggering downstream signaling cascades. The receptor helps mediate processes including glycogen synthesis lipid metabolism and protein synthesis through its influence on glucose transport and metabolic enzyme activity. The interaction with insulin generates a conformational change in the receptor which propagates cellular signal transduction essential for metabolic homeostasis.

Pathways

INSTA forms part of the insulin signaling pathway and the PI3K/Akt pathway. These pathways are essential for cellular glucose uptake and ensure energy balance within the body. Insulin receptor interacts with proteins like IRS-1 and PI3K which play roles in mediating insulin's effects. As part of these pathways INSTA assists in the convergence of signals that regulate anabolic and catabolic processes within cells making it integral to maintaining normal metabolic functions.

Associated diseases and disorders

INSTA holds a significant connection to conditions such as type 2 diabetes mellitus and insulin resistance. Both linked to misregulation or dysregulation of insulin receptor functions these disorders underline the importance of the insulin receptor in maintaining glucose homeostasis within the body. Moreover INSTA's association with other proteins such as IRS-1 becomes particularly apparent in these disease states as malfunctioning of these proteins can impair the insulin signaling cascade exacerbating metabolic syndrome. Understanding INSTA's role provides insight into therapeutic targets for managing these related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression cell line: T-47D (PMID:1617668, 29099049).

    The 220 kDa band is the pro-insulin receptor, while the 135 kDa band is the insulin receptor alpha subunit (PMID: 28765322, 6389544).

    Exposure time: 136 seconds

    All lanes: Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689) at 1/1000 dilution

    Lane 1: IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate at 20 µg

    Lane 2: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 156 kDa

    Observed band size: 135 kDa, 220 kDa

  • Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The 220 kDa band is the pro-insulin receptor, while the 135 kDa band is the insulin receptor alpha subunit (PMID: 28765322, 6389544).

    Exposure time: 114 seconds

    All lanes: Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689) at 1/1000 dilution

    Lane 1: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

    Lane 3: Mouse liver tissue lysate at 20 µg

    Lane 4: AR42J (rat pancreatic tumor epithelial cell), whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Lane 6: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 156 kDa

    Observed band size: 220 kDa, 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (1.08 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (1.08 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining mainly on blood vessels of human renal carcinoma (PMID: 20182859). The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (1.08 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast cancer (PMID: 17221153). The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver (PMID: 2688753). The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunoprecipitation - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Insulin Receptor alpha was immunoprecipitated from 0.35 mg IM-9 (human multiple myeloma b lymphoblast) whole cell lysate with ab283689 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283689 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: IM-9 (human multiple myeloma b lymphoblast) whole cell lysate 10 ug

    Lane 2: ab2833689 IP in IM-9 whole cell lysate 10 ug

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab283689 in IM-9 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 128 second.

    All lanes: Immunoprecipitation - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Predicted band size: 156 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat liver. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on leydig cells of rat testis. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor alpha antibody [EPR23962-157] (ab283689)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IM-9 cells labelling Insulin Receptor alpha with ab283689 at 1/50 (10.8 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membrane staining in IM-9 cells. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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