Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free
- BOND RX™ Validated
- RabMAb
- Recombinant
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Rabbit Recombinant Monoclonal Insulin Receptor antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.
View Alternative Names
CD220, Insulin receptor, IR
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human renal carcinoma tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (1.08 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining mainly on blood vessels of human renal carcinoma (PMID : 20182859). The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (1.08 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IM-9 cells labelling Insulin Receptor alpha with ab283689 at 1/50 (10.8 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membrane staining in IM-9 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (1.08 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human breast cancer (PMID : 17221153). The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Insulin Receptor alpha was immunoprecipitated from 0.35 mg IM-9 (human multiple myeloma b lymphoblast) whole cell lysate with ab283689 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283689 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : IM-9 (human multiple myeloma b lymphoblast) whole cell lysate 10 ug
Lane 2 : ab2833689 IP in IM-9 whole cell lysate 10 ug
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283689 in IM-9 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 128 second.
All lanes:
Immunoprecipitation - Anti-Insulin Receptor alpha antibody [EPR23962-157] (<a href='/en-us/products/primary-antibodies/insulin-receptor-alpha-antibody-epr23962-157-ab283689'>ab283689</a>)
Predicted band size: 156 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver (PMID : 2688753). The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse colon. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on leydig cells of rat testis. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labelling Insulin Receptor alpha with ab283689 at 1/2000 (0.54 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat liver. The section was incubated with ab283689 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- WB
Lab
Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Low expression cell line : T-47D (PMID : 1617668, 29099049).
The 220 kDa band is the pro-insulin receptor, while the 135 kDa band is the insulin receptor alpha subunit (PMID : 28765322, 6389544).
Exposure time : 136 seconds
All lanes:
Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (<a href='/en-us/products/primary-antibodies/insulin-receptor-alpha-antibody-epr23962-157-ab283689'>ab283689</a>) at 1/1000 dilution
Lane 1:
IM-9 (human multiple myeloma B Lymphoblast), whole cell lysate at 20 µg
Lane 2:
T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 156 kDa
Observed band size: 135 kDa
false
- WB
Lab
Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] - BSA and Azide free (AB283717)
This data was developed using ab283689, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The 220 kDa band is the pro-insulin receptor, while the 135 kDa band is the insulin receptor alpha subunit (PMID : 28765322, 6389544).
Exposure time : 114 seconds
All lanes:
Western blot - Anti-Insulin Receptor alpha antibody [EPR23962-157] (<a href='/en-us/products/primary-antibodies/insulin-receptor-alpha-antibody-epr23962-157-ab283689'>ab283689</a>) at 1/1000 dilution
Lane 1:
Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg
Lane 2:
C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg
Lane 3:
Mouse liver tissue lysate at 20 µg
Lane 4:
AR42J (rat pancreatic tumor epithelial cell), whole cell lysate at 20 µg
Lane 5:
NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 6:
HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 156 kDa
Observed band size: 220 kDa
false
Related conjugates and formulations (1)
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Anti-Insulin Receptor alpha antibody [EPR23962-157]
Reactivity data
Product details
ab283717 is the carrier-free version of ab283689.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
INSTA plays a critical role in glucose uptake and regulation of energy metabolism. It is an extracellular receptor involved in the formation of a tetrameric complex when interacting with insulin triggering downstream signaling cascades. The receptor helps mediate processes including glycogen synthesis lipid metabolism and protein synthesis through its influence on glucose transport and metabolic enzyme activity. The interaction with insulin generates a conformational change in the receptor which propagates cellular signal transduction essential for metabolic homeostasis.
Pathways
INSTA forms part of the insulin signaling pathway and the PI3K/Akt pathway. These pathways are essential for cellular glucose uptake and ensure energy balance within the body. Insulin receptor interacts with proteins like IRS-1 and PI3K which play roles in mediating insulin's effects. As part of these pathways INSTA assists in the convergence of signals that regulate anabolic and catabolic processes within cells making it integral to maintaining normal metabolic functions.
Product protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com