Anti-Insulin Receptor antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor antibody (AB137747)

Immunofluorescence analysis of RT-4 cells fixed in 4% paraformaldehyde at RT for 15 min labelling Insulin Receptor beta protein at cytoplasm and cell membrane using ab137747 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor antibody (AB137747)

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue labelling Insulin receptor protein at membrane with ab137747 at 1/500 dilution.
Antigen Retrieval : Trilogy� (EDTA based, pH 8.0) buffer

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

HepG2 whole cell extracts at 30 µg

Lane 2:

T-47D whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 156 kDa

false

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 156 kDa

false

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

shRNA Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 156 kDa

false

• WB

Lab

Western blot - Anti-Insulin Receptor antibody (AB137747)

False colour image of Western blot : Anti-Insulin Receptor antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137747 was shown to bind specifically to Insulin Receptor. A band was observed at 85/200 kDa in wild-type HepG2 cell lysates with no signal observed at this size in INSR knockout cell line ab282827 (knockout cell lysate ab283051). Pro-form of INSR is observed at 200 kDa and the mature form observed at 90 kDa. To generate this image, wild-type and INSR knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human INSR knockout Hep G2 cell lysate (ab283051)

Lane 2:

INSR knockout HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HDLM-2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 156 kDa

Observed band size: 85 kDa,200 kDa

false

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

Mouse pancrease tissue extracts at 50 µg

Lane 2:

Mouse eye tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 156 kDa

false

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

5% SDS PAGE

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

All lanes:

NIH-3T3 at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 156 kDa

false

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

5% SDS PAGE

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

PC-12 at 50 µg

Lane 2:

Rat2 at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 156 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor antibody (AB137747)

Immunohistochemical analysis of paraffin-embedded gastric cancer tissue labelling INSR protein at cytosol with ab137747 at 1/500 dilution
Antigen Retrieval : Trilogy� (EDTA based, pH 8.0) buffer

• WB

Supplier Data

Western blot - Anti-Insulin Receptor antibody (AB137747)

Samples were separated by 5 % SDS-PAGE.

All lanes:

Western blot - Anti-Insulin Receptor antibody (ab137747) at 1/1000 dilution

Lane 1:

Zebrafish brain tissue extracts at 30 µg

Lane 2:

Zebrafish liver tissue extracts at 30 µg

Predicted band size: 156 kDa

false