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Rabbit Recombinant Monoclonal Insulin Receptor phospho Y1361 antibody. Suitable for Dot, IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Synthetic peptide - Human, Human, Rat, Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (AB303492), expandable thumbnail
  • Immunoprecipitation - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (AB303492), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (AB303492), expandable thumbnail
  • Dot Blot - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (AB303492), expandable thumbnail
  • Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (AB303492), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Expected
Tested
Tested
Tested
Not recommended
Tested
Mouse
Expected
Expected
Expected
Expected
Not recommended
Tested
Rat
Expected
Expected
Expected
Expected
Not recommended
Tested
Synthetic peptide - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Synthetic peptide - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Target data

Function

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosine residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. In adipocytes, inhibits lipolysis (By similarity).

Alternative names

Insulin receptor, IR, INSR

Recommended products

Rabbit Recombinant Monoclonal Insulin Receptor phospho Y1361 antibody. Suitable for Dot, IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Synthetic peptide - Human, Human, Rat, Mouse samples.

Alternative names

Insulin receptor, IR, INSR

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26130-37

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293 (human embryonic kidney epithelial cell) cells labeling Insulin Receptor beta (phospho Y1361) with ab303492 at 1/500 dilution (0.986 ug/ml), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).

    Confocal image showing cytoplasmic staining in HEK-293 cells treated with pervanadate (1 mM) for 30 min and the signal decreased after phosphatase treatment at 37°C for 2 h.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/ml).

  • Immunoprecipitation - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Immunoprecipitation - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Insulin Receptor beta (phospho Y1361) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) treated with 1mM pervanadate for 30min whole cell lysate 10 µg with ab303492 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303492 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: 293T treated with 1mM pervanadate for 30min whole cell lysate 10 µg

    Lane 2: ab303492 IP in 293T treated with 1mM pervanadate for 30min whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab303492 in 293T treated with 1mM pervanadate for 30min whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 102 seconds.

    All lanes: Immunoprecipitation - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492) at 1/1000 dilution

    Lane 1: 293T treated with 1mM pervanadate for 30min whole cell lysate at 5 µg

    Lane 2: 293T treated with 1mM pervanadate for 30min whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 95 kDa

    Exposure time: 102s

  • Flow Cytometry (Intracellular) - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (human embryonic kidney epithelial cell) treated with 100nM insulin and 1mM pervanadate for 15 min (Red) / Untreated control (Green) cells labeling Insulin Receptor beta (phospho Y1361) with ab303492 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Dot Blot - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Dot Blot - Anti-Insulin Receptor (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Dot blot analysis of Insulin Receptor beta (phospho Y1361) using ab303492 at 1:1000 dilution (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Exposure time: 180 seconds

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Lane 1: Insulin Receptor beta (phospho Y1361 + T1362) peptide

    Lane 2: Insulin Receptor beta (phospho T1362) peptide

    Lane 3: Insulin Receptor beta (phospho Y1361) peptide a

    Lane 4: Insulin Receptor beta (phospho Y1361) peptide b

    Lane 5: Insulin Receptor beta non-phospho peptide corresponding to the region of peptides a and b

  • Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Buffer/dilution concentration: 5% NFDM/TBST

    Exposure time: 37 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27087690; PMID: 26297026; PMID: 6315728; PMID: 28915606 ).

    Bands of 95 kDa and ~45 kDa respectively represent the full length and the proteolytic cleavage fragment (β1) of the insulin receptor beta subunit (PMID: 6693383; PMID: 6315728; PMID: 28915606).

    This blot was developed using a high sensitvity ECL substrate.

    All lanes: Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492) at 1/1000 dilution

    Lane 1: Untreated C6 whole cell lysate (untreated membrane) at 20 µg

    Lane 2: C6 treated with 1mM pervanadate for 30min whole cell lysate (untreated membrane) at 20 µg

    Lane 3: C6 treated with 1mM pervanadate for 30min whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 45 kDa, 95 kDa, 210 kDa

    Exposure time: 103s

  • Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Buffer/dilution concentration: 5% NFDM/TBST

    Exposure time: 37 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27087690; PMID: 26297026; PMID: 6315728; PMID: 28915606 ).

    Bands of 95 kDa and ~45 kDa respectively represent the full length and the proteolytic cleavage fragment (β1) of the insulin receptor beta subunit (PMID: 6693383; PMID: 6315728; PMID: 28915606).

    All lanes: Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 whole cell lysate (untreated membrane) at 20 µg

    Lane 2: NIH/3T3 treated with 1mM pervanadate for 30min whole cell lysate (untreated membrane) at 20 µg

    Lane 3: NIH/3T3 treated with 1mM pervanadate for 30min whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 95 kDa, 210 kDa

    Exposure time: 37s

  • Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Buffer/dilution concentration: 5% NFDM/TBST

    Exposure time: 48 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 27087690; PMID: 6315728; PMID: 28915606).

    Bands of 95kDa and ~45 kDa respectively represent the full length and the proteolytic cleavage fragment (β1) of the insulin receptor beta subunit (PMID: 6693383; PMID: 6315728; PMID: 28915606).

    All lanes: Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492) at 1/1000 dilution

    Lane 1: Untreated 293T (human embryonic kidney epithelial cell) whole cell lysate (untreated membrane) at 20 µg

    Lane 2: 293T treated with 1mM pervanadate for 30min whole cell lysate (untreated membrane) at 20 µg

    Lane 3: 293T treated with 1mM pervanadate for 30min whole cell lysate (phosphatase treated membrane) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 95 kDa, 210 kDa

    Exposure time: 48s

  • Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492), expandable thumbnail

    Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492)

    Buffer/dilution: 5% NFDM/TBST

    Exposure time: 3 seconds.

    All lanes: Western blot - Anti-Insulin Receptor beta (phospho Y1361) antibody [EPR26130-37] (ab303492) at 1/1000 dilution

    Lane 1: Untreated 293T cells transfected with a human INSR (aa763-1382) expression vector containing a myc-His-tag® whole cell lysate at 5 µg

    Lane 2: 293T cells transfected with a human INSR (aa763-1382) expression vector containing a myc-His-tag®, treated with 1mM pervanadate for 30min whole cell lysate at 5 µg

    Lane 3: Untreated 293T cells transfected with a human INSR (mutation Y1361A) (aa763-1382) expression vector containing a myc-His-tag® whole cell lysate at 5 µg

    Lane 4: 293T cells transfected with a human INSR (mutation Y1361A) (aa763-1382) expression vector containing a myc-His-tag®, treated with 1mM pervanadate for 30min whole cell lysate at 5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 45 kDa, 95 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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